Construct: ORF ccsbBroad304_14704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019027.1_s304c1, BRDN0000459826
- Derived from:
- ccsbBroadEn_14704
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MUSK (4593)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14704
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4593 | MUSK | muscle associated receptor ... | NM_005592.4 | 99.9% | 100% | 375T>C |
| 2 | human | 4593 | MUSK | muscle associated receptor ... | XM_005251996.3 | 99% | 98.9% | 375T>C;1360_1361ins24 |
| 3 | human | 4593 | MUSK | muscle associated receptor ... | XM_005251994.3 | 98.8% | 98.8% | 375T>C;628_657del |
| 4 | human | 4593 | MUSK | muscle associated receptor ... | XM_005251995.3 | 97.9% | 97.8% | 375T>C;628_657del;1390_1391ins24 |
| 5 | human | 4593 | MUSK | muscle associated receptor ... | NM_001166281.2 | 88.9% | 88.8% | 375T>C;918_919ins264;1096_1097ins24 |
| 6 | human | 4593 | MUSK | muscle associated receptor ... | XM_017014734.1 | 88.8% | 88.8% | 375T>C;628_657del;948_949ins264 |
| 7 | human | 4593 | MUSK | muscle associated receptor ... | NM_001166280.2 | 87.9% | 87.8% | (many diffs) |
| 8 | human | 4593 | MUSK | muscle associated receptor ... | XM_011518708.2 | 52.5% | 52.5% | 0_1ins1236 |
| 9 | human | 4593 | MUSK | muscle associated receptor ... | NM_001369398.1 | 51.6% | 51.5% | 0_1ins1236;124_125ins24 |
| 10 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_010944.2 | 87.9% | 93% | (many diffs) |
| 11 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037130.1 | 87.1% | 92.4% | (many diffs) |
| 12 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037128.1 | 86.9% | 92% | (many diffs) |
| 13 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037129.1 | 86.1% | 91.3% | (many diffs) |
| 14 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | XM_006537659.2 | 85.9% | 91% | (many diffs) |
| 15 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037127.2 | 85.4% | 90.4% | (many diffs) |
| 16 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | XM_006537661.2 | 85.1% | 90.3% | (many diffs) |
| 17 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001165996.1 | 47.1% | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2676
- ORF length:
- 2607
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagagagctc gtcaacattc cactggtaca tattcttact ctggttgcct 121 tcagcggaac tgagaaactt ccaaaagctc ctgtcatcac cactcctctt gaaacagtgg 181 atgccttagt tgaagaagtg gctactttca tgtgtgcagt ggaatcctac ccccagcctg 241 agatttcctg gactagaaat aaaattctca ttaaactctt tgacacccgg tacagcatcc 301 gggagaatgg gcagctcctc accatcctga gtgtggaaga cagtgatgat ggcatttact 361 gctgcacggc caacaatggt gtgggaggag ctgtggagag ttgtggagcc ctgcaagtga 421 agatgaaacc taaaataact cgccctccca taaatgtgaa aataatagag ggattaaaag 481 cagtcctacc atgtactaca atgggtaatc ccaaaccatc agtgtcttgg ataaagggag 541 acagccctct cagggaaaat tcccgaattg cagttcttga atctgggagc ttgaggattc 601 ataacgtaca aaaggaagat gcaggacagt atcgatgtgt ggcaaaaaac agcctcggga 661 cagcatattc caaagtggtg aagctggaag ttgaggtttt tgccaggatc ctgcgggctc 721 ctgaatccca caatgtcacc tttggctcct ttgtgaccct gcactgtaca gcaacaggca 781 ttcctgtccc caccatcacc tggattgaaa acggaaatgc tgtttcttct gggtccattc 841 aagagagtgt gaaagaccga gtgattgact caagactgca gctgtttatc accaagccag 901 gactctacac atgcatagct accaataagc atggggagaa gttcagtact gccaaggctg 961 cagccaccat cagcatagca gaatggagta aaccacagaa agataacaaa ggctactgcg 1021 cccagtacag aggggaggtg tgtaatgcag tcctggcaaa agatgctctt gtttttctca 1081 acacctccta tgcggaccct gaggaggccc aagagctact ggtccacacg gcctggaatg 1141 aactgaaagt agtgagccca gtctgccggc cagctgctga ggctttgttg tgtaaccaca 1201 tcttccagga gtgcagtcct ggagtagtgc ctactcctat tcccatttgc agagagtact 1261 gcttggcagt aaaggagctc ttctgcgcaa aagaatggct ggtaatggaa gagaagaccc 1321 acagaggact ctacagatcc gagatgcatt tgctgtccgt gccagaatgc agcaagcttc 1381 ccagcatgca ttgggacccc acggcctgtg ccagactgcc acatctagat tataacaaag 1441 aaaacctaaa aacattccca ccaatgacgt cctcaaagcc aagtgtggac attccaaatc 1501 tgccttcctc ctcctcttct tccttctctg tctcacctac atactccatg actgtaataa 1561 tctccatcat gtccagcttt gcaatatttg tgcttcttac cataactact ctctattgct 1621 gccgaagaag aaaacaatgg aaaaataaga aaagagaatc agcagcagta accctcacca 1681 cactgccttc tgagctctta ctagatagac ttcatcccaa ccccatgtac cagaggatgc 1741 cgctccttct gaaccccaaa ttgctcagcc tggagtatcc aaggaataac attgaatatg 1801 tgagagacat cggagaggga gcgtttggaa gggtgtttca agcaagggca ccaggcttac 1861 ttccctatga acctttcact atggtggcag taaagatgct caaagaagaa gcctcggcag 1921 atatgcaagc ggactttcag agggaggcag ccctcatggc agaatttgac aaccctaaca 1981 ttgtgaagct attaggagtg tgtgctgtcg ggaagccaat gtgcctgctc tttgaataca 2041 tggcctatgg tgacctcaat gagttcctcc gcagcatgtc ccctcacacc gtgtgcagcc 2101 tcagtcacag tgacttgtct atgagggctc aggtctccag ccctgggccc ccacccctct 2161 cctgtgctga gcagctttgc attgccaggc aggtggcagc tggcatggct tacctctcag 2221 aacgtaagtt tgttcaccga gatttagcca ccaggaactg cctggtgggc gagaacatgg 2281 tggtgaaaat tgccgacttt ggcctctcca ggaacatcta ctcagcagac tactacaaag 2341 ctaatgaaaa cgacgctatc cctatccgtt ggatgccacc agagtccatt ttttataacc 2401 gctacactac agagtctgat gtgtgggcct atggcgtggt cctctgggag atcttctcct 2461 atggcctgca gccctactat gggatggccc atgaggaggt catttactac gtgcgagatg 2521 gcaacatcct ctcctgccct gagaactgcc ccgtggagct gtacaatctc atgcgtctat 2581 gttggagcaa gctgcctgca gacagaccca gtttcaccag tattcaccga attctggaac 2641 gcatgtgtga gagggcagag ggaactgtga gtgtcttgcc aactttcttg tacaaagtgg 2701 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2761 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta