Construct: ORF ccsbBroad304_14704

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019027.1_s304c1, BRDN0000459826
Derived from:
ccsbBroadEn_14704
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MUSK (4593)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14704
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4593 MUSK muscle associated receptor ... NM_005592.4 99.9% 100% 375T>C
2 human 4593 MUSK muscle associated receptor ... XM_005251996.3 99% 98.9% 375T>C;1360_1361ins24
3 human 4593 MUSK muscle associated receptor ... XM_005251994.3 98.8% 98.8% 375T>C;628_657del
4 human 4593 MUSK muscle associated receptor ... XM_005251995.3 97.9% 97.8% 375T>C;628_657del;1390_1391ins24
5 human 4593 MUSK muscle associated receptor ... NM_001166281.2 88.9% 88.8% 375T>C;918_919ins264;1096_1097ins24
6 human 4593 MUSK muscle associated receptor ... XM_017014734.1 88.8% 88.8% 375T>C;628_657del;948_949ins264
7 human 4593 MUSK muscle associated receptor ... NM_001166280.2 87.9% 87.8% (many diffs)
8 human 4593 MUSK muscle associated receptor ... XM_011518708.2 52.5% 52.5% 0_1ins1236
9 human 4593 MUSK muscle associated receptor ... NM_001369398.1 51.6% 51.5% 0_1ins1236;124_125ins24
10 mouse 18198 Musk muscle, skeletal, receptor ... NM_010944.2 87.9% 93% (many diffs)
11 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037130.1 87.1% 92.4% (many diffs)
12 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037128.1 86.9% 92% (many diffs)
13 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037129.1 86.1% 91.3% (many diffs)
14 mouse 18198 Musk muscle, skeletal, receptor ... XM_006537659.2 85.9% 91% (many diffs)
15 mouse 18198 Musk muscle, skeletal, receptor ... NM_001037127.2 85.4% 90.4% (many diffs)
16 mouse 18198 Musk muscle, skeletal, receptor ... XM_006537661.2 85.1% 90.3% (many diffs)
17 mouse 18198 Musk muscle, skeletal, receptor ... NM_001165996.1 47.1% 49.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2676
ORF length:
2607
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagagagctc gtcaacattc cactggtaca tattcttact ctggttgcct
121tcagcggaac tgagaaactt ccaaaagctc ctgtcatcac cactcctctt gaaacagtgg
181atgccttagt tgaagaagtg gctactttca tgtgtgcagt ggaatcctac ccccagcctg
241agatttcctg gactagaaat aaaattctca ttaaactctt tgacacccgg tacagcatcc
301gggagaatgg gcagctcctc accatcctga gtgtggaaga cagtgatgat ggcatttact
361gctgcacggc caacaatggt gtgggaggag ctgtggagag ttgtggagcc ctgcaagtga
421agatgaaacc taaaataact cgccctccca taaatgtgaa aataatagag ggattaaaag
481cagtcctacc atgtactaca atgggtaatc ccaaaccatc agtgtcttgg ataaagggag
541acagccctct cagggaaaat tcccgaattg cagttcttga atctgggagc ttgaggattc
601ataacgtaca aaaggaagat gcaggacagt atcgatgtgt ggcaaaaaac agcctcggga
661cagcatattc caaagtggtg aagctggaag ttgaggtttt tgccaggatc ctgcgggctc
721ctgaatccca caatgtcacc tttggctcct ttgtgaccct gcactgtaca gcaacaggca
781ttcctgtccc caccatcacc tggattgaaa acggaaatgc tgtttcttct gggtccattc
841aagagagtgt gaaagaccga gtgattgact caagactgca gctgtttatc accaagccag
901gactctacac atgcatagct accaataagc atggggagaa gttcagtact gccaaggctg
961cagccaccat cagcatagca gaatggagta aaccacagaa agataacaaa ggctactgcg
1021cccagtacag aggggaggtg tgtaatgcag tcctggcaaa agatgctctt gtttttctca
1081acacctccta tgcggaccct gaggaggccc aagagctact ggtccacacg gcctggaatg
1141aactgaaagt agtgagccca gtctgccggc cagctgctga ggctttgttg tgtaaccaca
1201tcttccagga gtgcagtcct ggagtagtgc ctactcctat tcccatttgc agagagtact
1261gcttggcagt aaaggagctc ttctgcgcaa aagaatggct ggtaatggaa gagaagaccc
1321acagaggact ctacagatcc gagatgcatt tgctgtccgt gccagaatgc agcaagcttc
1381ccagcatgca ttgggacccc acggcctgtg ccagactgcc acatctagat tataacaaag
1441aaaacctaaa aacattccca ccaatgacgt cctcaaagcc aagtgtggac attccaaatc
1501tgccttcctc ctcctcttct tccttctctg tctcacctac atactccatg actgtaataa
1561tctccatcat gtccagcttt gcaatatttg tgcttcttac cataactact ctctattgct
1621gccgaagaag aaaacaatgg aaaaataaga aaagagaatc agcagcagta accctcacca
1681cactgccttc tgagctctta ctagatagac ttcatcccaa ccccatgtac cagaggatgc
1741cgctccttct gaaccccaaa ttgctcagcc tggagtatcc aaggaataac attgaatatg
1801tgagagacat cggagaggga gcgtttggaa gggtgtttca agcaagggca ccaggcttac
1861ttccctatga acctttcact atggtggcag taaagatgct caaagaagaa gcctcggcag
1921atatgcaagc ggactttcag agggaggcag ccctcatggc agaatttgac aaccctaaca
1981ttgtgaagct attaggagtg tgtgctgtcg ggaagccaat gtgcctgctc tttgaataca
2041tggcctatgg tgacctcaat gagttcctcc gcagcatgtc ccctcacacc gtgtgcagcc
2101tcagtcacag tgacttgtct atgagggctc aggtctccag ccctgggccc ccacccctct
2161cctgtgctga gcagctttgc attgccaggc aggtggcagc tggcatggct tacctctcag
2221aacgtaagtt tgttcaccga gatttagcca ccaggaactg cctggtgggc gagaacatgg
2281tggtgaaaat tgccgacttt ggcctctcca ggaacatcta ctcagcagac tactacaaag
2341ctaatgaaaa cgacgctatc cctatccgtt ggatgccacc agagtccatt ttttataacc
2401gctacactac agagtctgat gtgtgggcct atggcgtggt cctctgggag atcttctcct
2461atggcctgca gccctactat gggatggccc atgaggaggt catttactac gtgcgagatg
2521gcaacatcct ctcctgccct gagaactgcc ccgtggagct gtacaatctc atgcgtctat
2581gttggagcaa gctgcctgca gacagaccca gtttcaccag tattcaccga attctggaac
2641gcatgtgtga gagggcagag ggaactgtga gtgtcttgcc aactttcttg tacaaagtgg
2701ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2761ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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