Construct: ORF ccsbBroad304_14714

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009942.2_s304c1, BRDN0000459492
Derived from:
ccsbBroadEn_14714
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NME1 (4830)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14714
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4830 NME1 NME/NM23 nucleoside diphosp... NM_000269.2 100% 100%
2 human 4830 NME1 NME/NM23 nucleoside diphosp... NM_198175.1 85.8% 85.8% 1_75del
3 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001018137.3 81.4% 88.1% (many diffs)
4 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001018138.1 81.4% 88.1% (many diffs)
5 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001018139.2 81.4% 88.1% (many diffs)
6 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_002512.4 81.4% 88.1% (many diffs)
7 human 654364 NME1-NME2 NME1-NME2 readthrough NM_001018136.3 53.8% 50.1% (many diffs)
8 human 654364 NME1-NME2 NME1-NME2 readthrough NR_037149.2 33.8% (many diffs)
9 mouse 18102 Nme1 NME/NM23 nucleoside diphosp... NM_008704.2 87.7% 94% (many diffs)
10 mouse 18102 Nme1 NME/NM23 nucleoside diphosp... XM_030245646.1 80.3% 86.1% (many diffs)
11 mouse 18103 Nme2 NME/NM23 nucleoside diphosp... NM_001077529.2 80% 88.1% (many diffs)
12 mouse 18103 Nme2 NME/NM23 nucleoside diphosp... NM_008705.5 80% 88.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
522
ORF length:
456
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc caactgtgag cgtaccttca ttgcgatcaa accagatggg gtccagcggg
121gtcttgtggg agagattatc aagcgttttg agcagaaagg attccgcctt gttggtctga
181aattcatgca agcttccgaa gatcttctca aggaacacta cgttgacctg aaggaccgtc
241cattctttgc cggcctggtg aaatacatgc actcagggcc ggtagttgcc atggtctggg
301aggggctgaa tgtggtgaag acgggccgag tcatgctcgg ggagaccaac cctgcagact
361ccaagcctgg gaccatccgt ggagacttct gcatacaagt tggcaggaac attatacatg
421gcagtgattc tgtggagagt gcagagaagg agatcggctt gtggtttcac cctgaggaac
481tggtagatta cacgagctgt gctcagaact ggatctatga atgcccaact ttcttgtaca
541aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
601ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)