Transcript: Mouse NM_008705.5

Mus musculus NME/NM23 nucleoside diphosphate kinase 2 (Nme2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Nme2 (18103)
Length:
1027
CDS:
436..894

Additional Resources:

NCBI RefSeq record:
NM_008705.5
NBCI Gene record:
Nme2 (18103)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008705.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024904 CGAAGAACTGATCGACTACAA pLKO.1 843 CDS 100% 4.950 2.970 N Nme2 n/a
2 TRCN0000295136 CGAAGAACTGATCGACTACAA pLKO_005 843 CDS 100% 4.950 2.970 N Nme2 n/a
3 TRCN0000024577 CGACCTGAAAGACCGTCCTTT pLKO.1 594 CDS 100% 4.950 2.970 N Gm5425 n/a
4 TRCN0000024905 CTGTGGTTTAAGCCCGAAGAA pLKO.1 829 CDS 100% 4.950 2.970 N Nme2 n/a
5 TRCN0000054520 AGTGCTGAGAAAGAGATCCAT pLKO.1 808 CDS 100% 3.000 1.800 N Nme2 n/a
6 TRCN0000301216 AGTGCTGAGAAAGAGATCCAT pLKO_005 808 CDS 100% 3.000 1.800 N Nme2 n/a
7 TRCN0000024907 CGAGATCATCAAACGGTTCGA pLKO.1 501 CDS 100% 2.640 1.584 N Nme2 n/a
8 TRCN0000295135 CGAGATCATCAAACGGTTCGA pLKO_005 501 CDS 100% 2.640 1.584 N Nme2 n/a
9 TRCN0000054519 AGAACACCTGAAGCAGCATTA pLKO.1 570 CDS 100% 10.800 5.400 Y Nme2 n/a
10 TRCN0000287721 AGAACACCTGAAGCAGCATTA pLKO_005 570 CDS 100% 10.800 5.400 Y Nme2 n/a
11 TRCN0000054521 AGACCAATCCAGCTGATTCAA pLKO.1 713 CDS 100% 5.625 2.813 Y Nme2 n/a
12 TRCN0000287720 AGACCAATCCAGCTGATTCAA pLKO_005 713 CDS 100% 5.625 2.813 Y Nme2 n/a
13 TRCN0000054518 GTTGGCAGGAACATCATTCAT pLKO.1 769 CDS 100% 5.625 2.813 Y Nme2 n/a
14 TRCN0000287722 GTTGGCAGGAACATCATTCAT pLKO_005 769 CDS 100% 5.625 2.813 Y Nme2 n/a
15 TRCN0000024906 ACATCATTCATGGCAGTGATT pLKO.1 779 CDS 100% 4.950 2.475 Y Nme2 n/a
16 TRCN0000024574 GCAGCATTACATCGACCTGAA pLKO.1 582 CDS 100% 4.050 2.025 Y Gm5425 n/a
17 TRCN0000024908 GCATTCAAGTTGGCAGGAACA pLKO.1 761 CDS 100% 4.050 2.025 Y Nme2 n/a
18 TRCN0000054522 GACTACAAGTCTTGTGCCCAT pLKO.1 856 CDS 100% 2.160 1.080 Y Nme2 n/a
19 TRCN0000379743 ATTCATGGCAGTGATTCAGTA pLKO_005 784 CDS 100% 4.950 2.475 Y NME2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008705.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14715 pDONR223 100% 90.3% 98% None (many diffs) n/a
2 ccsbBroad304_14715 pLX_304 0% 90.3% 98% V5 (many diffs) n/a
3 TRCN0000469993 CCAAGGCATCGCGTCTCAAAGTAA pLX_317 72.2% 90.3% 98% V5 (many diffs) n/a
4 TRCN0000492213 TGTACTCCCGGGACTCAGACCACG pLX_317 92.3% 90.3% 98% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_01101 pDONR223 100% 80% 88.1% None (many diffs) n/a
6 ccsbBroad304_01101 pLX_304 0% 80% 88.1% V5 (many diffs) n/a
7 TRCN0000468949 CACCGCTGGGTGCTAGCGAGCCCG pLX_317 73.3% 80% 88.1% V5 (many diffs) n/a
8 ccsbBroadEn_14714 pDONR223 0% 80% 88.1% None (many diffs) n/a
9 ccsbBroad304_14714 pLX_304 0% 80% 88.1% V5 (many diffs) n/a
10 TRCN0000479668 TTTTAGCACTCTCCAGTATATATA pLX_317 69.3% 80% 88.1% V5 (many diffs) n/a
11 TRCN0000488282 CTTCCTTTCGAGACTCGATGACCG pLX_317 63.2% 80% 88.1% V5 (not translated due to prior stop codon) (many diffs) n/a
12 TRCN0000470841 CGTGCGTGAACAATGGCCCGGCTC pLX_317 40.9% 51.3% 13.8% V5 (not translated due to prior stop codon) (many diffs) n/a
13 ccsbBroadEn_15341 pDONR223 100% 51.1% 13.8% None (many diffs) n/a
14 ccsbBroad304_15341 pLX_304 0% 51.1% 13.8% V5 (not translated due to prior stop codon) (many diffs) n/a
15 ccsbBroadEn_10663 pDONR223 100% 47% 51% None (many diffs) n/a
16 ccsbBroad304_10663 pLX_304 0% 47% 51% V5 (many diffs) n/a
17 TRCN0000476918 CCTGTCGTTTGGTGACAACTCTGT pLX_317 33.8% 47% 51% V5 (many diffs) n/a
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