Construct: ORF ccsbBroad304_14786

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016090.2_s304c1, BRDN0000459705
Derived from:
ccsbBroadEn_14786
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAR2A (5576)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14786
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5576 PRKAR2A protein kinase cAMP-depende... NM_001321983.2 100% 100%
2 human 5576 PRKAR2A protein kinase cAMP-depende... NM_001321982.2 94.5% 94.5% 873_938del
3 human 5576 PRKAR2A protein kinase cAMP-depende... NM_004157.4 94.5% 94.5% 873_938del
4 human 5576 PRKAR2A protein kinase cAMP-depende... XM_011533942.3 94.5% 94.5% 873_938del
5 human 5576 PRKAR2A protein kinase cAMP-depende... NM_001321989.2 87.6% 87.6% 350_351ins84;789_854del
6 human 5576 PRKAR2A protein kinase cAMP-depende... XM_005265315.4 84% 77% (many diffs)
7 human 5576 PRKAR2A protein kinase cAMP-depende... XM_011533943.2 82.8% 77.9% (many diffs)
8 human 5576 PRKAR2A protein kinase cAMP-depende... XR_002959546.1 67.3% 1_237del;1110_1175del;1450_1701del
9 human 5576 PRKAR2A protein kinase cAMP-depende... XR_002959547.1 38.1% (many diffs)
10 mouse 19087 Prkar2a protein kinase, cAMP depend... NM_008924.2 81.6% 81.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag ccacatccag atcccgccgg ggctcacgga gctgctgcag ggctacacgg
121tggaggtgct gcgacagcag ccgcctgacc tcgtcgaatt cgcagtggag tacttcaccc
181gcctgcgcga ggcccgcgcc ccagcctcag tcctgcccgc cgccacccca cgccagagcc
241tgggccaccc cccgccagaa cccggcccgg accgtgtcgc cgacgccaaa ggggacagcg
301agtcggagga ggacgaggac ttggaagttc cagttcctag cagatttaat agacgagtat
361cagtctgtgc tgagacctat aaccctgatg aggaagagga agatacagat ccaagggtga
421ttcatcctaa aactgatgaa cagagatgca gacttcagga agcttgcaaa gatattctcc
481ttttcaaaaa tcttgatcag gaacagcttt ctcaagttct cgatgccatg tttgaaagga
541tagtcaaagc tgatgagcat gtcattgacc aaggagatga tggagacaac ttttatgtca
601tagaacgggg aacttatgac attttagtaa caaaagataa tcaaacccgc tctgttggtc
661aatatgacaa ccgtggcagt tttggagaac tagctctgat gtacaacacc ccgagagctg
721ctaccattgt tgctacctca gaaggctccc tttggggact ggaccgggtg acttttagaa
781gaatcatagt gaaaaataat gcaaagaaga ggaagatgtt tgaatcattt attgagtctg
841tgcccctcct taaatcacta gaggtgtcag aacgaatgaa gattgtggat gtaataggag
901agaagatcta taaggatgga gaacgcataa tcactcagac taaatcaaac aaggatggtg
961ggaaccagga ggtcgagatt gcccgctgcc ataaggggca gtactttgga gagcttgccc
1021tggtcaccaa caaacccaga gctgcctcag cttatgcagt tggagatgtc aaatgcttag
1081ttatggatgt acaagcattc gagaggcttc tggggccctg catggacatc atgaagagga
1141acatctcaca ctatgaggaa cagctggtga agatgtttgg ctccagcgtg gatctgggca
1201acctcgggca gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1261tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1321caatcaacct ctggatta

Download FASTA (ORF) (Full)