Construct: ORF ccsbBroad304_14940

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018922.1_s304c1, BRDN0000459406
Derived from:
ccsbBroadEn_14940
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAP4K4 (9448)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14940
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9448 MAP4K4 mitogen-activated protein k... XM_017005372.1 99.9% 19.6% 685delG
2 human 9448 MAP4K4 mitogen-activated protein k... NM_004834.5 99.8% 19.7% 685delG;1715_1716insCAG
3 human 9448 MAP4K4 mitogen-activated protein k... XM_017005373.1 99.7% 19.7% 685delG;1968_1969insGGCGAAGTG
4 human 9448 MAP4K4 mitogen-activated protein k... XM_017005374.1 99.2% 19.8% 685delG;3093_3094ins24
5 human 9448 MAP4K4 mitogen-activated protein k... XM_017005375.1 99% 19.8% 685delG;1968_1969insGGCGAAGTG;3084_3085ins24
6 human 9448 MAP4K4 mitogen-activated protein k... XM_017005369.1 97.2% 19.1% 685delG;1482_1574del;1808_1809insCAG
7 human 9448 MAP4K4 mitogen-activated protein k... XM_006712869.2 96.7% 19.3% 685delG;1482_1574del;3186_3187ins24
8 human 9448 MAP4K4 mitogen-activated protein k... XM_017005370.1 96.6% 19.3% (many diffs)
9 human 9448 MAP4K4 mitogen-activated protein k... XM_017005371.1 96.4% 19.3% (many diffs)
10 human 9448 MAP4K4 mitogen-activated protein k... XM_005264071.2 96.3% 19.3% (many diffs)
11 human 9448 MAP4K4 mitogen-activated protein k... NM_145687.4 94.8% 18.9% 685delG;1610_1771del;3255_3256ins24
12 human 9448 MAP4K4 mitogen-activated protein k... XM_017005368.1 94.8% 18.9% (many diffs)
13 human 9448 MAP4K4 mitogen-activated protein k... XM_005264065.3 94.7% 18.6% 685delG;2273_2464del
14 human 9448 MAP4K4 mitogen-activated protein k... XM_005264066.3 94.6% 18.6% 685delG;1715_1716insCAG;2270_2461del
15 human 9448 MAP4K4 mitogen-activated protein k... XM_005264069.2 94.6% 18.9% (many diffs)
16 human 9448 MAP4K4 mitogen-activated protein k... XM_005264068.3 94.5% 18.7% 685delG;1968_1969insGGCGAAGTG;2264_2455del
17 human 9448 MAP4K4 mitogen-activated protein k... XM_006712868.2 94.1% 18.7% 685delG;2273_2464del;3285_3286ins24
18 human 9448 MAP4K4 mitogen-activated protein k... XM_017005366.1 93.7% 18.4% 685delG;1611_1841del
19 human 9448 MAP4K4 mitogen-activated protein k... NM_001242560.2 93.1% 18.5% 685delG;1611_1841del;3324_3325ins24
20 human 9448 MAP4K4 mitogen-activated protein k... XM_017005367.1 93% 18.6% (many diffs)
21 human 9448 MAP4K4 mitogen-activated protein k... XM_017005365.1 92.5% 18.4% (many diffs)
22 human 9448 MAP4K4 mitogen-activated protein k... XM_005264062.3 92.4% 18.2% 685delG;1482_1574del;2366_2557del
23 human 9448 MAP4K4 mitogen-activated protein k... XM_005264063.3 92.3% 18.2% (many diffs)
24 human 9448 MAP4K4 mitogen-activated protein k... NM_001242559.2 92.2% 18.5% (many diffs)
25 human 9448 MAP4K4 mitogen-activated protein k... XM_005264064.3 92.2% 18.2% (many diffs)
26 human 9448 MAP4K4 mitogen-activated protein k... XM_017005363.1 91.8% 18.3% (many diffs)
27 human 9448 MAP4K4 mitogen-activated protein k... XM_017005359.1 91.4% 18% 685delG;1482_1574del;1704_1934del
28 human 9448 MAP4K4 mitogen-activated protein k... XM_017005364.1 91.4% 18.3% (many diffs)
29 human 9448 MAP4K4 mitogen-activated protein k... XM_017005360.1 91.4% 18% (many diffs)
30 human 9448 MAP4K4 mitogen-activated protein k... XM_017005361.1 90.8% 18.1% (many diffs)
31 human 9448 MAP4K4 mitogen-activated protein k... XM_017005362.1 90.7% 18.1% (many diffs)
32 human 9448 MAP4K4 mitogen-activated protein k... XM_005264059.3 90.7% 17.8% 685delG;1610_1771del;2435_2626del
33 human 9448 MAP4K4 mitogen-activated protein k... XM_017005356.1 90.5% 17.9% (many diffs)
34 human 9448 MAP4K4 mitogen-activated protein k... XM_005264060.3 90.4% 17.9% (many diffs)
35 human 9448 MAP4K4 mitogen-activated protein k... XM_017005357.1 90.1% 17.9% (many diffs)
36 human 9448 MAP4K4 mitogen-activated protein k... XM_017005358.1 90% 18% (many diffs)
37 human 9448 MAP4K4 mitogen-activated protein k... NM_145686.3 89.9% 18% (many diffs)
38 human 9448 MAP4K4 mitogen-activated protein k... XM_006712865.2 89.1% 17.5% 685delG;1611_1841del;2504_2695del
39 human 9448 MAP4K4 mitogen-activated protein k... XM_005264057.3 88.6% 17.4% (many diffs)
40 human 9448 MAP4K4 mitogen-activated protein k... XM_017005354.1 88.5% 17.6% (many diffs)
41 human 9448 MAP4K4 mitogen-activated protein k... XM_005264058.3 88.4% 17.5% (many diffs)
42 human 9448 MAP4K4 mitogen-activated protein k... XM_017005355.1 88.3% 17.7% (many diffs)
43 human 9448 MAP4K4 mitogen-activated protein k... XM_017005351.1 88.3% 17.5% (many diffs)
44 human 9448 MAP4K4 mitogen-activated protein k... XM_017005352.1 88% 17.5% (many diffs)
45 human 9448 MAP4K4 mitogen-activated protein k... XM_017005353.1 87.9% 17.5% (many diffs)
46 human 9448 MAP4K4 mitogen-activated protein k... XM_005264050.3 87.1% 17.1% (many diffs)
47 human 9448 MAP4K4 mitogen-activated protein k... XM_005264052.3 87% 17.1% (many diffs)
48 human 9448 MAP4K4 mitogen-activated protein k... XM_017005350.1 86.5% 17.2% (many diffs)
49 human 9448 MAP4K4 mitogen-activated protein k... XM_017005349.1 84.9% 16.9% (many diffs)
50 human 9448 MAP4K4 mitogen-activated protein k... XM_005264048.2 83.1% 16.5% (many diffs)
51 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496046.1 89.2% 19.8% (many diffs)
52 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496045.1 85.4% 18.9% (many diffs)
53 mouse 26921 Map4k4 mitogen-activated protein k... NM_001252202.1 85.2% 19% (many diffs)
54 mouse 26921 Map4k4 mitogen-activated protein k... NM_001252201.1 84.7% 18.8% (many diffs)
55 mouse 26921 Map4k4 mitogen-activated protein k... NM_008696.2 83.8% 18.6% (many diffs)
56 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496044.1 83% 18.5% (many diffs)
57 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321018.1 82.6% 18.3% (many diffs)
58 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321021.1 82.4% 18.3% (many diffs)
59 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321016.1 81.1% 18% (many diffs)
60 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496043.1 80.9% 18% (many diffs)
61 mouse 26921 Map4k4 mitogen-activated protein k... NM_001252200.1 80.3% 17.8% (many diffs)
62 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321013.1 79.7% 17.6% (many diffs)
63 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321011.1 79.2% 17.5% (many diffs)
64 mouse 26921 Map4k4 mitogen-activated protein k... XM_011238530.1 78.4% 17.4% (many diffs)
65 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321010.1 78.2% 17.4% (many diffs)
66 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321004.1 77.9% 17.3% (many diffs)
67 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496042.1 77.8% 17.2% (many diffs)
68 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321002.1 76.5% 16.9% (many diffs)
69 mouse 26921 Map4k4 mitogen-activated protein k... XM_017320998.1 74.9% 16.6% (many diffs)
70 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496041.1 74.8% 16.6% (many diffs)
71 mouse 26921 Map4k4 mitogen-activated protein k... XM_017320994.1 74.7% 16.6% (many diffs)
72 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321001.1 74.6% 16.6% (many diffs)
73 mouse 26921 Map4k4 mitogen-activated protein k... XR_001784730.1 57.4% (many diffs)
74 mouse 26921 Map4k4 mitogen-activated protein k... XR_001784729.1 51.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
783
ORF length:
714
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgaacgac tcccctgcaa aaagtctggt ggacatcgac ctctcctccc
121tgcgggatcc tgctgggatt tttgagctgg tggaagtggt tggaaatggc acctatggac
181aagtctataa gggtcgacat gttaaaacgg gtcagttggc agccatcaaa gttatggatg
241tcactgagga tgaagaggaa gaaatcaaac tggagataaa tatgctaaag aaatactctc
301atcacagaaa cattgcaaca tattatggtg ctttcatcaa aaagagccct ccaggacatg
361atgaccaact ctggcttgtt atggagttct gtggggctgg gtccattaca gaccttgtga
421agaacaccaa agggaacaca ctcaaagaag actggatcgc ttacatctcc agagaaatcc
481tgaggggact ggcacatctt cacattcatc atgtgattca ccgggatatc aagggccaga
541atgtgttgct gactgagaat gcagaggtga aacttgttga ctttggtgtg agtgctcagc
601tggacaggac tgtggggcgg agaaatacgt tcataggcac tccctactgg atggctcctg
661aggtcatcgc ctgtgatgag aacccagatg ccacctatga ttacagaagt gatctttggt
721cttgtggcat tacagccatt gagatggcag aagtgctccc cctctctgtg acatgcatcc
781aatgagagca ctgtttctca ttcccagaaa ccctcctccc cggctgaagt caaaaaaatg
841gtcgaagaag ttttttagtt ttatagaagg gtgcctggtg aagaattaca tgcagcggcc
901ctctacagag cagcttttga aacatccttt tataagggat cagccaaatg aaaggcaagt
961tagaatccag cttaaggatc atatagatcg taccaggaag aagagaggcg agaaagatga
1021aactgagtat gagtacagtg ggagtgagga agaagaggag gaagtgcctg aacaggaagg
1081agagccaagt tccattgtga acgtgcctgg tgagtctact cttcgccgag atttcctgag
1141actgcagcag gagaacaagg aacgttccga ggctcttcgg agacaacagt tactacagga
1201gcaacagctc cgggagcagg aagaatataa aaggcaactg ctggcagaga gacagaagcg
1261gattgagcag cagaaagaac agaggcgacg gctagaagag caacaaagga gagagcggga
1321agctagaagg cagcaggaac gtgaacagcg aaggagagaa caagaagaaa agaggcgtct
1381agaggagttg gagagaaggc gcaaagaaga agaggagagg agacgggcag aagaagaaaa
1441gaggagagtt gaaagagaac aggagtatat caggcgacag ctagaagagg agcagcggca
1501cttggaagtc cttcagcagc agctgctcca ggagcaggcc atgttactgc atgaccatag
1561gaggccgcac ccgcagcact cgcagcagcc gccaccaccg cagcaggaaa ggagcaagcc
1621aagcttccat gctcccgagc ccaaagccca ctacgagcct gctgaccgag cgcgagaggt
1681tcctgtgaga acaacatctc gctcccctgt tctgtcccgt cgagattccc cactgcaggg
1741cagtgggcag cagaatagcc aggcaggaca gagaaactcc accagcagta ttgagcccag
1801gcttctgtgg gagagagtgg agaagctggt gcccagacct ggcagtggca gctcctcagg
1861gtccagcaac tcaggatccc agcccgggtc tcaccctggg tctcagagtg gctccgggga
1921acgcttcaga gtgagatcat catccaagtc tgaaggctct ccatctcagc gcctggaaaa
1981tgcagtgaaa aaacctgaag ataaaaagga agttttcaga cccctcaagc ctgctggcga
2041agtggatctg accgcactgg ccaaagagct tcgagcagtg gaagatgtac ggccacctca
2101caaagtaacg gactactcct catccagtga ggagtcgggg acgacggatg aggaggacga
2161cgatgtggag caggaagggg ctgacgagtc cacctcagga ccagaggaca ccagagcagc
2221gtcatctctg aatttgagca atggtgaaac ggaatctgtg aaaaccatga ttgtccatga
2281tgatgtagaa agtgagccgg ccatgacccc atccaaggag ggcactctaa tcgtccgcca
2341gactcagtcc gctagtagca cactccagaa acacaaatct tcctcctcct ttacaccttt
2401tatagacccc agattactac agatttctcc atctagcgga acaacagtga catctgtggt
2461gggattttcc tgtgatggga tgagaccaga agccataagg caagatccta cccggaaagg
2521ctcagtggtc aatgtgaatc ctaccaacac taggccacag agtgacaccc cggagattcg
2581taaatacaag aagaggttta actctgagat tctgtgtgct gccttatggg gagtgaattt
2641gctagtgggt acagagagtg gcctgatgct gctggacaga agtggccaag ggaaggtcta
2701tcctcttatc aaccgaagac gatttcaaca aatggacgta cttgagggct tgaatgtctt
2761ggtgacaata tctggcaaaa aggataagtt acgtgtctac tatttgtcct ggttaagaaa
2821taaaatactt cacaatgatc cagaagttga gaagaagcag ggatggacaa ccgtagggga
2881tttggaagga tgtgtacatt ataaagttgt aaaatatgaa agaatcaaat ttctggtgat
2941tgctttgaag agttctgtgg aagtctatgc gtgggcacca aagccatatc acaaatttat
3001ggcctttaag tcatttggag aattggtaca taagccatta ctggtggatc tcactgttga
3061ggaaggccag aggttgaaag tgatctatgg atcctgtgct ggattccatg ctgttgatgt
3121ggattcagga tcagtctatg acatttatct accaacacat gtaagaaaga acccacactc
3181tatgatccag tgtagcatca aaccccatgc aatcatcatc ctccccaata cagatggaat
3241ggagcttctg gtgtgctatg aagatgaggg ggtttatgta aacacatatg gaaggatcac
3301caaggatgta gttctacagt ggggagagat gcctacatca gtagcatata ttcgatccaa
3361tcagacaatg ggctggggag agaaggccat agagatccga tctgtggaaa ctggtcactt
3421ggatggtgtg ttcatgcaca aaagggctca aagactaaaa ttcttgtgtg aacgcaatga
3481caaggtgttc tttgcctctg ttcggtctgg tggcagcagt caggtttatt tcatgacctt
3541aggcaggact tctcttctga gctggttgcc aactttcttg tacaaagtgg ttggtaagcc
3601tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg
3661gcgcgttaag tcgacaatca acctctggat ta

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