Construct: ORF ccsbBroad304_14940
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018922.1_s304c1, BRDN0000459406
- Derived from:
- ccsbBroadEn_14940
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP4K4 (9448)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14940
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005372.1 | 99.9% | 19.6% | 685delG |
2 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_004834.5 | 99.8% | 19.7% | 685delG;1715_1716insCAG |
3 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005373.1 | 99.7% | 19.7% | 685delG;1968_1969insGGCGAAGTG |
4 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005374.1 | 99.2% | 19.8% | 685delG;3093_3094ins24 |
5 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005375.1 | 99% | 19.8% | 685delG;1968_1969insGGCGAAGTG;3084_3085ins24 |
6 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005369.1 | 97.2% | 19.1% | 685delG;1482_1574del;1808_1809insCAG |
7 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_006712869.2 | 96.7% | 19.3% | 685delG;1482_1574del;3186_3187ins24 |
8 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005370.1 | 96.6% | 19.3% | (many diffs) |
9 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005371.1 | 96.4% | 19.3% | (many diffs) |
10 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264071.2 | 96.3% | 19.3% | (many diffs) |
11 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_145687.4 | 94.8% | 18.9% | 685delG;1610_1771del;3255_3256ins24 |
12 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005368.1 | 94.8% | 18.9% | (many diffs) |
13 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264065.3 | 94.7% | 18.6% | 685delG;2273_2464del |
14 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264066.3 | 94.6% | 18.6% | 685delG;1715_1716insCAG;2270_2461del |
15 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264069.2 | 94.6% | 18.9% | (many diffs) |
16 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264068.3 | 94.5% | 18.7% | 685delG;1968_1969insGGCGAAGTG;2264_2455del |
17 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_006712868.2 | 94.1% | 18.7% | 685delG;2273_2464del;3285_3286ins24 |
18 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005366.1 | 93.7% | 18.4% | 685delG;1611_1841del |
19 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_001242560.2 | 93.1% | 18.5% | 685delG;1611_1841del;3324_3325ins24 |
20 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005367.1 | 93% | 18.6% | (many diffs) |
21 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005365.1 | 92.5% | 18.4% | (many diffs) |
22 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264062.3 | 92.4% | 18.2% | 685delG;1482_1574del;2366_2557del |
23 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264063.3 | 92.3% | 18.2% | (many diffs) |
24 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_001242559.2 | 92.2% | 18.5% | (many diffs) |
25 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264064.3 | 92.2% | 18.2% | (many diffs) |
26 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005363.1 | 91.8% | 18.3% | (many diffs) |
27 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005359.1 | 91.4% | 18% | 685delG;1482_1574del;1704_1934del |
28 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005364.1 | 91.4% | 18.3% | (many diffs) |
29 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005360.1 | 91.4% | 18% | (many diffs) |
30 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005361.1 | 90.8% | 18.1% | (many diffs) |
31 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005362.1 | 90.7% | 18.1% | (many diffs) |
32 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264059.3 | 90.7% | 17.8% | 685delG;1610_1771del;2435_2626del |
33 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005356.1 | 90.5% | 17.9% | (many diffs) |
34 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264060.3 | 90.4% | 17.9% | (many diffs) |
35 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005357.1 | 90.1% | 17.9% | (many diffs) |
36 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005358.1 | 90% | 18% | (many diffs) |
37 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_145686.3 | 89.9% | 18% | (many diffs) |
38 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_006712865.2 | 89.1% | 17.5% | 685delG;1611_1841del;2504_2695del |
39 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264057.3 | 88.6% | 17.4% | (many diffs) |
40 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005354.1 | 88.5% | 17.6% | (many diffs) |
41 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264058.3 | 88.4% | 17.5% | (many diffs) |
42 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005355.1 | 88.3% | 17.7% | (many diffs) |
43 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005351.1 | 88.3% | 17.5% | (many diffs) |
44 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005352.1 | 88% | 17.5% | (many diffs) |
45 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005353.1 | 87.9% | 17.5% | (many diffs) |
46 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264050.3 | 87.1% | 17.1% | (many diffs) |
47 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264052.3 | 87% | 17.1% | (many diffs) |
48 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005350.1 | 86.5% | 17.2% | (many diffs) |
49 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005349.1 | 84.9% | 16.9% | (many diffs) |
50 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264048.2 | 83.1% | 16.5% | (many diffs) |
51 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496046.1 | 89.2% | 19.8% | (many diffs) |
52 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496045.1 | 85.4% | 18.9% | (many diffs) |
53 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_001252202.1 | 85.2% | 19% | (many diffs) |
54 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_001252201.1 | 84.7% | 18.8% | (many diffs) |
55 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_008696.2 | 83.8% | 18.6% | (many diffs) |
56 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496044.1 | 83% | 18.5% | (many diffs) |
57 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321018.1 | 82.6% | 18.3% | (many diffs) |
58 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321021.1 | 82.4% | 18.3% | (many diffs) |
59 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321016.1 | 81.1% | 18% | (many diffs) |
60 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496043.1 | 80.9% | 18% | (many diffs) |
61 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_001252200.1 | 80.3% | 17.8% | (many diffs) |
62 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321013.1 | 79.7% | 17.6% | (many diffs) |
63 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321011.1 | 79.2% | 17.5% | (many diffs) |
64 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_011238530.1 | 78.4% | 17.4% | (many diffs) |
65 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321010.1 | 78.2% | 17.4% | (many diffs) |
66 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321004.1 | 77.9% | 17.3% | (many diffs) |
67 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496042.1 | 77.8% | 17.2% | (many diffs) |
68 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321002.1 | 76.5% | 16.9% | (many diffs) |
69 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017320998.1 | 74.9% | 16.6% | (many diffs) |
70 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496041.1 | 74.8% | 16.6% | (many diffs) |
71 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017320994.1 | 74.7% | 16.6% | (many diffs) |
72 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321001.1 | 74.6% | 16.6% | (many diffs) |
73 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XR_001784730.1 | 57.4% | (many diffs) | |
74 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XR_001784729.1 | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 783
- ORF length:
- 714
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaacgac tcccctgcaa aaagtctggt ggacatcgac ctctcctccc 121 tgcgggatcc tgctgggatt tttgagctgg tggaagtggt tggaaatggc acctatggac 181 aagtctataa gggtcgacat gttaaaacgg gtcagttggc agccatcaaa gttatggatg 241 tcactgagga tgaagaggaa gaaatcaaac tggagataaa tatgctaaag aaatactctc 301 atcacagaaa cattgcaaca tattatggtg ctttcatcaa aaagagccct ccaggacatg 361 atgaccaact ctggcttgtt atggagttct gtggggctgg gtccattaca gaccttgtga 421 agaacaccaa agggaacaca ctcaaagaag actggatcgc ttacatctcc agagaaatcc 481 tgaggggact ggcacatctt cacattcatc atgtgattca ccgggatatc aagggccaga 541 atgtgttgct gactgagaat gcagaggtga aacttgttga ctttggtgtg agtgctcagc 601 tggacaggac tgtggggcgg agaaatacgt tcataggcac tccctactgg atggctcctg 661 aggtcatcgc ctgtgatgag aacccagatg ccacctatga ttacagaagt gatctttggt 721 cttgtggcat tacagccatt gagatggcag aagtgctccc cctctctgtg acatgcatcc 781 aatgagagca ctgtttctca ttcccagaaa ccctcctccc cggctgaagt caaaaaaatg 841 gtcgaagaag ttttttagtt ttatagaagg gtgcctggtg aagaattaca tgcagcggcc 901 ctctacagag cagcttttga aacatccttt tataagggat cagccaaatg aaaggcaagt 961 tagaatccag cttaaggatc atatagatcg taccaggaag aagagaggcg agaaagatga 1021 aactgagtat gagtacagtg ggagtgagga agaagaggag gaagtgcctg aacaggaagg 1081 agagccaagt tccattgtga acgtgcctgg tgagtctact cttcgccgag atttcctgag 1141 actgcagcag gagaacaagg aacgttccga ggctcttcgg agacaacagt tactacagga 1201 gcaacagctc cgggagcagg aagaatataa aaggcaactg ctggcagaga gacagaagcg 1261 gattgagcag cagaaagaac agaggcgacg gctagaagag caacaaagga gagagcggga 1321 agctagaagg cagcaggaac gtgaacagcg aaggagagaa caagaagaaa agaggcgtct 1381 agaggagttg gagagaaggc gcaaagaaga agaggagagg agacgggcag aagaagaaaa 1441 gaggagagtt gaaagagaac aggagtatat caggcgacag ctagaagagg agcagcggca 1501 cttggaagtc cttcagcagc agctgctcca ggagcaggcc atgttactgc atgaccatag 1561 gaggccgcac ccgcagcact cgcagcagcc gccaccaccg cagcaggaaa ggagcaagcc 1621 aagcttccat gctcccgagc ccaaagccca ctacgagcct gctgaccgag cgcgagaggt 1681 tcctgtgaga acaacatctc gctcccctgt tctgtcccgt cgagattccc cactgcaggg 1741 cagtgggcag cagaatagcc aggcaggaca gagaaactcc accagcagta ttgagcccag 1801 gcttctgtgg gagagagtgg agaagctggt gcccagacct ggcagtggca gctcctcagg 1861 gtccagcaac tcaggatccc agcccgggtc tcaccctggg tctcagagtg gctccgggga 1921 acgcttcaga gtgagatcat catccaagtc tgaaggctct ccatctcagc gcctggaaaa 1981 tgcagtgaaa aaacctgaag ataaaaagga agttttcaga cccctcaagc ctgctggcga 2041 agtggatctg accgcactgg ccaaagagct tcgagcagtg gaagatgtac ggccacctca 2101 caaagtaacg gactactcct catccagtga ggagtcgggg acgacggatg aggaggacga 2161 cgatgtggag caggaagggg ctgacgagtc cacctcagga ccagaggaca ccagagcagc 2221 gtcatctctg aatttgagca atggtgaaac ggaatctgtg aaaaccatga ttgtccatga 2281 tgatgtagaa agtgagccgg ccatgacccc atccaaggag ggcactctaa tcgtccgcca 2341 gactcagtcc gctagtagca cactccagaa acacaaatct tcctcctcct ttacaccttt 2401 tatagacccc agattactac agatttctcc atctagcgga acaacagtga catctgtggt 2461 gggattttcc tgtgatggga tgagaccaga agccataagg caagatccta cccggaaagg 2521 ctcagtggtc aatgtgaatc ctaccaacac taggccacag agtgacaccc cggagattcg 2581 taaatacaag aagaggttta actctgagat tctgtgtgct gccttatggg gagtgaattt 2641 gctagtgggt acagagagtg gcctgatgct gctggacaga agtggccaag ggaaggtcta 2701 tcctcttatc aaccgaagac gatttcaaca aatggacgta cttgagggct tgaatgtctt 2761 ggtgacaata tctggcaaaa aggataagtt acgtgtctac tatttgtcct ggttaagaaa 2821 taaaatactt cacaatgatc cagaagttga gaagaagcag ggatggacaa ccgtagggga 2881 tttggaagga tgtgtacatt ataaagttgt aaaatatgaa agaatcaaat ttctggtgat 2941 tgctttgaag agttctgtgg aagtctatgc gtgggcacca aagccatatc acaaatttat 3001 ggcctttaag tcatttggag aattggtaca taagccatta ctggtggatc tcactgttga 3061 ggaaggccag aggttgaaag tgatctatgg atcctgtgct ggattccatg ctgttgatgt 3121 ggattcagga tcagtctatg acatttatct accaacacat gtaagaaaga acccacactc 3181 tatgatccag tgtagcatca aaccccatgc aatcatcatc ctccccaata cagatggaat 3241 ggagcttctg gtgtgctatg aagatgaggg ggtttatgta aacacatatg gaaggatcac 3301 caaggatgta gttctacagt ggggagagat gcctacatca gtagcatata ttcgatccaa 3361 tcagacaatg ggctggggag agaaggccat agagatccga tctgtggaaa ctggtcactt 3421 ggatggtgtg ttcatgcaca aaagggctca aagactaaaa ttcttgtgtg aacgcaatga 3481 caaggtgttc tttgcctctg ttcggtctgg tggcagcagt caggtttatt tcatgacctt 3541 aggcaggact tctcttctga gctggttgcc aactttcttg tacaaagtgg ttggtaagcc 3601 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg 3661 gcgcgttaag tcgacaatca acctctggat ta