Construct: ORF ccsbBroad304_15066

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011924.2_s304c1, BRDN0000459606
Derived from:
ccsbBroadEn_15066
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAG2 (51422)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15066
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51422 PRKAG2 protein kinase AMP-activate... NM_001040633.1 99.8% 100% 462G>A;772C>A
2 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012270.1 99.8% 100% 462G>A;772C>A
3 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012271.2 99.6% 99.8% 462G>A;620_621insAGC;769C>A
4 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012272.1 99.6% 99.8% 462G>A;620_621insAGC;769C>A
5 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516283.1 92.8% 92.9% 1_120del;582G>A;892C>A
6 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516284.1 92.6% 92.7% (many diffs)
7 human 51422 PRKAG2 protein kinase AMP-activate... NM_016203.4 92.1% 92.2% 1_132del;594G>A;904C>A
8 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012269.1 91.9% 92% (many diffs)
9 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250004.4 90.1% 89.4% (many diffs)
10 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446786.1 90.1% 89.4% (many diffs)
11 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012268.2 89.9% 89.2% (many diffs)
12 human 51422 PRKAG2 protein kinase AMP-activate... XM_006716021.2 84.2% 83.6% (many diffs)
13 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516282.1 84.1% 83.4% (many diffs)
14 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250002.4 83.7% 83.1% (many diffs)
15 human 51422 PRKAG2 protein kinase AMP-activate... NM_001363698.1 80.4% 79.9% (many diffs)
16 human 51422 PRKAG2 protein kinase AMP-activate... NM_001304527.1 80.2% 79.7% (many diffs)
17 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250006.5 72.5% 71.2% (many diffs)
18 human 51422 PRKAG2 protein kinase AMP-activate... NM_001304531.1 62.4% 62.4% 0_1ins591;181C>A
19 human 51422 PRKAG2 protein kinase AMP-activate... NM_024429.1 62.4% 62.4% 0_1ins591;181C>A
20 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446789.1 62.4% 62.4% 0_1ins591;181C>A
21 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012276.2 62.2% 62.2% 0_1ins591;29_30insAGC;178C>A
22 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012277.2 60.7% 60.5% (many diffs)
23 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012278.1 58.6% 58.6% 0_1ins651;121C>A
24 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012279.2 58.6% 58.6% 0_1ins651;121C>A
25 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012280.2 58.6% 58.6% 0_1ins651;121C>A
26 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012281.2 58.6% 58.6% 0_1ins651;121C>A
27 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516285.2 56% 55.3% (many diffs)
28 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446787.1 56% 55.3% (many diffs)
29 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012274.2 55.8% 55.1% (many diffs)
30 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446788.1 55.8% 55.1% (many diffs)
31 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516286.2 54.5% 53.6% (many diffs)
32 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516287.1 52.5% 51.9% (many diffs)
33 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012275.2 52.5% 51.9% (many diffs)
34 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535612.3 89.6% 94% (many diffs)
35 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535611.3 82.7% 86.8% (many diffs)
36 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_145401.2 82.5% 86.6% (many diffs)
37 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001310480.1 74.5% 75.5% (many diffs)
38 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535614.3 71.4% 74.4% (many diffs)
39 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_017320595.1 70.2% 73.4% (many diffs)
40 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001170555.1 57.2% 61.5% (many diffs)
41 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001170556.1 57% 61.3% (many diffs)
42 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_011249740.1 56.2% 60.1% (many diffs)
43 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_017320596.1 56.2% 60% (many diffs)
44 mouse 108099 Prkag2 protein kinase, AMP-activat... XR_880821.2 37.5% (many diffs)
45 mouse 108099 Prkag2 protein kinase, AMP-activat... XR_389738.3 36.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1641
ORF length:
1575
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gctcctggac ggagacctgg agggttccgg aaagcattcc tctcgaaagg
121tggacagccc cttcggcccg ggcagcccct ccaaagggtt cttctccaga ggcccccagc
181cccggccctc cagccccatg tctgcacctg tgaggcccaa gaccagcccc ggctctccca
241aaaccgtgtt cccgttctcc taccaggagt ccccgccacg ctcccctcga cgcatgagct
301tcagtgggat cttccgctcc tcctccaaag agtcttcccc caactccaac cctgctacct
361cgcccggggg catcaggttt ttctcccgct ccagaaaaac ctccggcctc tcctcctctc
421cgtcaacacc cacccaagtg accaagcagc acacgtttcc cctggaatcc tataagcacg
481agcctgaacg gttagagaat cgcatctatg cctcgtcttc ccccccagac acagggcaga
541ggttctgccc gtcttccttc cagagcccga ccaggcctcc actggcatca ccgacacact
601atgctccctc caaagccgcg gcgctggcgg cggccctggg acccgcggaa gccggcatgc
661tggagaagct ggagttcgag gacgaagcag tagaagactc agaaagtggt gtttacatgc
721gattcatgag gtcacacaag tgttatgaca tcgttccaac cagttcaaag cttgttgtct
781ttgatactac attacaagtt aaaaaggcct tctttgcttt ggtagccaac ggtgtcagag
841cagcgccact gtgggagagt aaaaaacaaa gttttgtagg aatgctaaca attacagatt
901tcataaatat actacataga tactataaat cacctatggt acagatttat gaattagagg
961aacataaaat tgaaacatgg agggagcttt atttacaaga aacatttaag cctttagtga
1021atatatctcc agatgcaagc ctcttcgatg ctgtatactc cttgatcaaa aataaaatcc
1081acagattgcc cgttattgac cctatcagtg ggaatgcact ttatatactt acccacaaaa
1141gaatcctcaa gttcctccag ctttttatgt ctgatatgcc aaagcctgcc ttcatgaagc
1201agaacctgga tgagcttgga ataggaacgt accacaacat tgccttcata catccagaca
1261ctcccatcat caaagccttg aacatatttg tggaaagacg aatatcagct ctgcctgttg
1321tggatgagtc aggaaaagtt gtagatattt attccaaatt tgatgtaatt aatcttgctg
1381ctgagaaaac atacaataac ctagatatca cggtgaccca ggcccttcag caccgttcac
1441agtattttga aggtgttgtg aagtgcaata agctggaaat actggagacc atcgtggaca
1501gaatagtaag agctgaggtc catcggctgg tggtggtaaa tgaagcagat agtattgtgg
1561gtattatttc cctgtcggac attctgcaag ccctgatcct cacaccagca ggtgccaaac
1621aaaaggagac agaaacggag tgcccaactt tcttgtacaa agtggttggt aagcctatcc
1681ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1741ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)