Transcript: Human NM_001304531.1

Homo sapiens protein kinase AMP-activated non-catalytic subunit gamma 2 (PRKAG2), transcript variant e, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
PRKAG2 (51422)
Length:
2176
CDS:
88..1074

Additional Resources:

NCBI RefSeq record:
NM_001304531.1
NBCI Gene record:
PRKAG2 (51422)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149343 AGGAGTATACAGCATCGAAG pXPR_003 AGG 389 39% 8 0.7174 PRKAG2 PRKAG2 77462
2 BRDN0001147689 TGGCGCTGCTCGGACACCGT pXPR_003 TGG 175 18% 4 0.4968 PRKAG2 PRKAG2 77461
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001304531.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430131 ATCCACAGATTGCCCGTTATT pLKO_005 508 CDS 100% 13.200 18.480 N PRKAG2 n/a
2 TRCN0000003145 CCTATGGTACAGATTTATGAA pLKO.1 364 CDS 100% 5.625 4.500 N PRKAG2 n/a
3 TRCN0000433732 AGATAGTATTGTGGGTATTAT pLKO_005 978 CDS 100% 15.000 10.500 N PRKAG2 n/a
4 TRCN0000434898 AGACTCAGAAAGTGGTGTTTA pLKO_005 126 CDS 100% 13.200 9.240 N PRKAG2 n/a
5 TRCN0000430309 ATGAGGTCACACAAGTGTTAT pLKO_005 157 CDS 100% 13.200 9.240 N PRKAG2 n/a
6 TRCN0000429506 ATTTGTGGAAAGACGAATATC pLKO_005 717 CDS 100% 13.200 9.240 N PRKAG2 n/a
7 TRCN0000195266 CTTCACCACATCCATGAATAA pLKO.1 1719 3UTR 100% 13.200 9.240 N PRKAG2 n/a
8 TRCN0000436467 TTTGTAGGAATGCTAACAATT pLKO_005 304 CDS 100% 13.200 9.240 N PRKAG2 n/a
9 TRCN0000003148 GAAGTGCAATAAGCTGGAAAT pLKO.1 891 CDS 100% 10.800 7.560 N PRKAG2 n/a
10 TRCN0000003146 TGGCTGCAAGTGGTTAAGAAT pLKO.1 1148 3UTR 100% 5.625 3.938 N PRKAG2 n/a
11 TRCN0000003147 GCCTTCATACATCCAGACACT pLKO.1 673 CDS 100% 2.640 1.584 N PRKAG2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001304531.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03303 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03303 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000473870 TCGCCAAGCTGGTAATCACGGCAG pLX_317 44.3% 100% 100% V5 n/a
4 ccsbBroadEn_08289 pDONR223 100% 62.4% 62.4% None 0_1ins591;181C>A n/a
5 ccsbBroad304_08289 pLX_304 0% 62.4% 62.4% V5 0_1ins591;181C>A n/a
6 TRCN0000470612 ACTCCGAGAATTGCACTTTTTGAA pLX_317 25.2% 62.4% 62.4% V5 0_1ins591;181C>A n/a
7 ccsbBroadEn_15066 pDONR223 0% 62.4% 62.4% None 0_1ins591;181C>A n/a
8 ccsbBroad304_15066 pLX_304 0% 62.4% 62.4% V5 0_1ins591;181C>A n/a
9 TRCN0000470682 AAGCATTACAACATGTAGGTAAAT pLX_317 27.2% 62.4% 62.4% V5 0_1ins591;181C>A n/a
10 TRCN0000488555 GGCGAATAATCCAGTCCTGCTCTC pLX_317 20.9% 57.6% 57.6% V5 (not translated due to prior stop codon) 0_1ins723 n/a
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