Transcript: Human XM_011516286.2

PREDICTED: Homo sapiens protein kinase AMP-activated non-catalytic subunit gamma 2 (PRKAG2), transcript variant X19, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRKAG2 (51422)
Length:
5520
CDS:
190..1302

Additional Resources:

NCBI RefSeq record:
XM_011516286.2
NBCI Gene record:
PRKAG2 (51422)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149343 AGGAGTATACAGCATCGAAG pXPR_003 AGG 365 33% 7 0.725 PRKAG2 PRKAG2 77462
2 BRDN0001147689 TGGCGCTGCTCGGACACCGT pXPR_003 TGG 151 14% 3 0.5442 PRKAG2 PRKAG2 77461
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011516286.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430131 ATCCACAGATTGCCCGTTATT pLKO_005 586 CDS 100% 13.200 18.480 N PRKAG2 n/a
2 TRCN0000003145 CCTATGGTACAGATTTATGAA pLKO.1 442 CDS 100% 5.625 4.500 N PRKAG2 n/a
3 TRCN0000433732 AGATAGTATTGTGGGTATTAT pLKO_005 1056 CDS 100% 15.000 10.500 N PRKAG2 n/a
4 TRCN0000434898 AGACTCAGAAAGTGGTGTTTA pLKO_005 204 CDS 100% 13.200 9.240 N PRKAG2 n/a
5 TRCN0000430309 ATGAGGTCACACAAGTGTTAT pLKO_005 235 CDS 100% 13.200 9.240 N PRKAG2 n/a
6 TRCN0000429506 ATTTGTGGAAAGACGAATATC pLKO_005 795 CDS 100% 13.200 9.240 N PRKAG2 n/a
7 TRCN0000195266 CTTCACCACATCCATGAATAA pLKO.1 5088 3UTR 100% 13.200 9.240 N PRKAG2 n/a
8 TRCN0000436467 TTTGTAGGAATGCTAACAATT pLKO_005 382 CDS 100% 13.200 9.240 N PRKAG2 n/a
9 TRCN0000003148 GAAGTGCAATAAGCTGGAAAT pLKO.1 969 CDS 100% 10.800 7.560 N PRKAG2 n/a
10 TRCN0000003146 TGGCTGCAAGTGGTTAAGAAT pLKO.1 4517 3UTR 100% 5.625 3.938 N PRKAG2 n/a
11 TRCN0000003147 GCCTTCATACATCCAGACACT pLKO.1 751 CDS 100% 2.640 1.584 N PRKAG2 n/a
12 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 3941 3UTR 100% 4.950 2.475 Y n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4014 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4014 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011516286.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03303 pDONR223 100% 82.9% 81.3% None (many diffs) n/a
2 ccsbBroad304_03303 pLX_304 0% 82.9% 81.3% V5 (many diffs) n/a
3 TRCN0000473870 TCGCCAAGCTGGTAATCACGGCAG pLX_317 44.3% 82.9% 81.3% V5 (many diffs) n/a
4 ccsbBroadEn_08289 pDONR223 100% 54.5% 53.6% None (many diffs) n/a
5 ccsbBroad304_08289 pLX_304 0% 54.5% 53.6% V5 (many diffs) n/a
6 TRCN0000470612 ACTCCGAGAATTGCACTTTTTGAA pLX_317 25.2% 54.5% 53.6% V5 (many diffs) n/a
7 ccsbBroadEn_15066 pDONR223 0% 54.5% 53.6% None (many diffs) n/a
8 ccsbBroad304_15066 pLX_304 0% 54.5% 53.6% V5 (many diffs) n/a
9 TRCN0000470682 AAGCATTACAACATGTAGGTAAAT pLX_317 27.2% 54.5% 53.6% V5 (many diffs) n/a
10 TRCN0000488555 GGCGAATAATCCAGTCCTGCTCTC pLX_317 20.9% 50.7% 49.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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