Construct: ORF ccsbBroadEn_03303

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002286.1_s300c1, BRDN0000385831
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAG2 (51422)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51422 PRKAG2 protein kinase AMP-activate... NM_001304531.1 100% 100%
2 human 51422 PRKAG2 protein kinase AMP-activate... NM_024429.1 100% 100%
3 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446789.1 100% 100%
4 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012276.2 99.6% 99.6% 29_30insAGC
5 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012277.2 97.3% 96.9% 0_1ins22;2T>G;6_6delCinsGAA
6 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012278.1 93.9% 93.9% 0_1ins60
7 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012279.2 93.9% 93.9% 0_1ins60
8 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012280.2 93.9% 93.9% 0_1ins60
9 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012281.2 93.9% 93.9% 0_1ins60
10 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516285.2 85.1% 83.9% (many diffs)
11 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446787.1 85.1% 83.9% (many diffs)
12 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012274.2 84.9% 83.7% (many diffs)
13 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446788.1 84.9% 83.7% (many diffs)
14 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516286.2 82.9% 81.3% (many diffs)
15 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516287.1 79.9% 78.7% (many diffs)
16 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012275.2 79.9% 78.7% (many diffs)
17 human 51422 PRKAG2 protein kinase AMP-activate... NM_001363698.1 73.7% 73.7% 1_351del
18 human 51422 PRKAG2 protein kinase AMP-activate... NM_001304527.1 73.4% 73.4% 1_351del;380_381insAGC
19 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250006.5 65.1% 64.2% (many diffs)
20 human 51422 PRKAG2 protein kinase AMP-activate... NM_001040633.1 62.4% 62.4% 1_591del
21 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012270.1 62.4% 62.4% 1_591del
22 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012271.2 62.2% 62.2% 1_591del;620_621insAGC
23 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012272.1 62.2% 62.2% 1_591del;620_621insAGC
24 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516283.1 58% 58% 1_711del
25 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516284.1 57.8% 57.8% 1_711del;740_741insAGC
26 human 51422 PRKAG2 protein kinase AMP-activate... NM_016203.4 57.6% 57.6% 1_723del
27 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012269.1 57.4% 57.4% 1_723del;752_753insAGC
28 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250004.4 56.1% 55.3% (many diffs)
29 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446786.1 56.1% 55.3% (many diffs)
30 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012268.2 55.9% 55.1% (many diffs)
31 human 51422 PRKAG2 protein kinase AMP-activate... XM_006716021.2 52.4% 51.7% (many diffs)
32 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516282.1 52.3% 51.6% (many diffs)
33 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250002.4 52.1% 51.4% (many diffs)
34 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001170555.1 91.7% 98.4% (many diffs)
35 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001170556.1 91.4% 98.1% (many diffs)
36 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_011249740.1 90% 96.3% (many diffs)
37 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_017320596.1 89.8% 96% (many diffs)
38 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535614.3 72.3% 77.6% (many diffs)
39 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001310480.1 67.7% 72.7% (many diffs)
40 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535612.3 57.4% 61.6% (many diffs)
41 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535611.3 52.9% 56.8% (many diffs)
42 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_145401.2 52.8% 56.6% (many diffs)
43 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_017320595.1 40.5% 43.4% (many diffs)
44 mouse 108099 Prkag2 protein kinase, AMP-activat... XR_880821.2 24% (many diffs)
45 mouse 108099 Prkag2 protein kinase, AMP-activat... XR_389738.3 23.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1050
ORF length:
984
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT GGAGAAGCTG GAGTTCGAGG ACGAAGCAGT AGAAGACTCA GAAAGTGGTG
121TTTACATGCG ATTCATGAGG TCACACAAGT GTTATGACAT CGTTCCAACC AGTTCAAAGC
181TTGTTGTCTT TGATACTACA TTACAAGTTA AAAAGGCCTT CTTTGCTTTG GTAGCCAACG
241GTGTCCGAGC AGCGCCACTG TGGGAGAGTA AAAAACAAAG TTTTGTAGGA ATGCTAACAA
301TTACAGATTT CATAAATATA CTACATAGAT ACTATAAATC ACCTATGGTA CAGATTTATG
361AATTAGAGGA ACATAAAATT GAAACATGGA GGGAGCTTTA TTTACAAGAA ACATTTAAGC
421CTTTAGTGAA TATATCTCCA GATGCAAGCC TCTTCGATGC TGTATACTCC TTGATCAAAA
481ATAAAATCCA CAGATTGCCC GTTATTGACC CTATCAGTGG GAATGCACTT TATATACTTA
541CCCACAAAAG AATCCTCAAG TTCCTCCAGC TTTTTATGTC TGATATGCCA AAGCCTGCCT
601TCATGAAGCA GAACCTGGAT GAGCTTGGAA TAGGAACGTA CCACAACATT GCCTTCATAC
661ATCCAGACAC TCCCATCATC AAAGCCTTGA ACATATTTGT GGAAAGACGA ATATCAGCTC
721TGCCTGTTGT GGATGAGTCA GGAAAAGTTG TAGATATTTA TTCCAAATTT GATGTAATTA
781ATCTTGCTGC TGAGAAAACA TACAATAACC TAGATATCAC GGTGACCCAG GCCCTTCAGC
841ACCGTTCACA GTATTTTGAA GGTGTTGTGA AGTGCAATAA GCTGGAAATA CTGGAGACCA
901TCGTGGACAG AATAGTAAGA GCTGAGGTCC ATCGGCTGGT GGTGGTAAAT GAAGCAGATA
961GTATTGTGGG TATTATTTCC CTGTCGGACA TTCTGCAAGC CCTGATCCTC ACACCAGCAG
1021GTGCCAAACA AAAGGAGACA GAAACGGAGT GCCCAACTTT CTTGTACAAA GTtggcatta
1081taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)