Construct: ORF ccsbBroadEn_06278

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002072.1_s300c1, BRDN0000386934
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GK (2710)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2710 GK glycerol kinase NM_203391.3 99.8% 99.6% 237T>A;391C>A;501C>A
2 human 2710 GK glycerol kinase NM_000167.5 98.6% 98.4% (many diffs)
3 human 2710 GK glycerol kinase XM_011545492.1 94.8% 94.6% (many diffs)
4 human 2710 GK glycerol kinase NM_001205019.1 94.3% 94.2% (many diffs)
5 human 2710 GK glycerol kinase XM_006724484.2 93.7% 93.5% (many diffs)
6 human 2710 GK glycerol kinase NM_001128127.2 93.3% 93.2% (many diffs)
7 human 2710 GK glycerol kinase XM_011545491.2 89.8% 89.7% (many diffs)
8 human 2710 GK glycerol kinase XM_006724483.2 88.8% 88.7% (many diffs)
9 human 2712 GK2 glycerol kinase 2 NM_033214.3 82.5% 82.1% (many diffs)
10 human 2713 GK3P glycerol kinase 3 pseudogene NR_026575.1 68.1% (many diffs)
11 human 2710 GK glycerol kinase XM_017029410.1 61.3% 61.3% 0_1ins615
12 human 2710 GK glycerol kinase XM_017029411.1 60.1% 60.1% 0_1ins615;112_113insTGAAAATCTCTCATAGCG
13 human 2710 GK glycerol kinase XM_017029412.2 60.1% 60.1% 0_1ins615;112_113insTGAAAATCTCTCATAGCG
14 human 2710 GK glycerol kinase XM_006724485.2 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
15 human 2710 GK glycerol kinase XM_006724486.3 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
16 human 2710 GK glycerol kinase XM_011545493.2 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
17 human 2710 GK glycerol kinase XM_011545494.2 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
18 human 2710 GK glycerol kinase XM_017029409.1 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
19 human 2710 GK glycerol kinase XM_005274488.4 56.8% 56.8% (many diffs)
20 mouse 14933 Gk glycerol kinase NM_001331046.1 91.4% 96.7% (many diffs)
21 mouse 14933 Gk glycerol kinase NM_008194.3 90.3% 95.8% (many diffs)
22 mouse 14933 Gk glycerol kinase XM_006527833.3 86.8% 91.9% (many diffs)
23 mouse 14933 Gk glycerol kinase NM_001294140.1 86.5% 91.5% (many diffs)
24 mouse 14933 Gk glycerol kinase NM_001294138.1 85.8% 91% (many diffs)
25 mouse 14933 Gk glycerol kinase NM_212444.2 85.5% 90.6% (many diffs)
26 mouse 14933 Gk glycerol kinase XM_006527829.3 82.3% 87.2% (many diffs)
27 mouse 14933 Gk glycerol kinase XM_006527831.3 81.4% 86.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1656
ORF length:
1590
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC AGCCTCAAAG AAGGCAGTTT TGGGGCCATT GGTGGGGGCG GTGGACCAGG
121GCACCAGTTC GACGCGCTTT TTGGTTTTCA ATTCAAAAAC AGCTGAACTA CTTAGTCATC
181ATCAAGTAGA AATAAAACAA GAGTTCCCAA GAGAAGGATG GGTGGAACAG GACCCTAAGG
241AAATTCTACA TTCTGTCTAT GAGTGTATAG AGAAAACATG TGAGAAACTT GGACAGCTCA
301AAATTGATAT TTCCAACATA AAAGCTATTG GTGTCAGCAA CCAGAGGGAA ACCACTGTAG
361TCTGGGACAA GATAACTGGA GAGCCTCTCT ACAATGCTGT GGTGTGGCTT GATCTAAGAA
421CCCAGTCTAC CGTTGAGAGT CTTAGTAAAA GAATTACAGG AAATAATAAC TTTGTCAAGT
481CCAAGACAGG CCTTCCACTT AGCACTTACT TCAGTGCAGT GAAACTTCGT TGGCTCCTTG
541ACAATGTGAG AAAAGTTCAA AAGGCAGTTG AAGAAAAACG AGCTCTTTTT GGGACTATTG
601ATTCATGGCT TATTTGGAGT TTGACAGGAG GAGTCAATGG AGGTGTCCAC TGTACAGATG
661TAACAAATGC AAGTAGGACT ATGCTTTTCA ACATTCATTC TTTGGAATGG GATAAACAAC
721TCTGCGAATT TTTTGGAATT CCAATGGAAA TTCTTCCAAA TGTCCGGAGT TCTTCTGAGA
781TCTATGGCCT AATGAAAATC TCTCATAGCG TGAAAGCTGG GGCCTTGGAA GGTGTGCCAA
841TATCTGGGTG TTTAGGGGAC CAGTCTGCTG CATTGGTGGG ACAAATGTGC TTCCAGATTG
901GACAAGCCAA AAATACGTAT GGAACAGGAT GTTTCTTACT ATGTAATACA GGCCATAAGT
961GTGTATTTTC TGATCATGGC CTTCTCACCA CAGTGGCTTA CAAACTTGGC AGAGACAAAC
1021CAGTATATTA TGCTTTGGAA GGTTCTGTAG CTATAGCTGG TGCTGTTATT CGCTGGCTAA
1081GAGACAATCT TGGAATTATA AAGACCTCAG AAGAAATTGA AAAACTTGCT AAAGAAGTAG
1141GTACTTCTTA TGGCTGCTAC TTCGTCCCAG CATTTTCGGG GTTATATGCA CCTTATTGGG
1201AGCCCAGCGC AAGAGGGATA ATCTGTGGAC TCACTCAGTT CACCAATAAA TGCCATATTG
1261CTTTTGCTGC ATTAGAAGCT GTTTGTTTCC AAACTCGAGA GATTTTGGAT GCCATGAATC
1321GAGACTGTGG AATTCCACTC AGTCATTTGC AGGTAGATGG AGGAATGACC AGCAACAAAA
1381TTCTTATGCA GCTACAAGCA GACATTCTGT ATATACCAGT AGTGAAGCCC TCAATGCCCG
1441AAACCACTGC ACTGGGTGCG GCTATGGCGG CAGGGGCTGC AGAAGGAGTC GGCGTATGGA
1501GTCTCGAACC CGAGGATTTG TCTGCCGTCA CGATGGAGCG GTTTGAACCT CAGATTAATG
1561CGGAGGAAAG TGAAATTCGT TATTCTACAT GGAAGAAAGC TGTGATGAAG TCAATGGGTT
1621GGGTTACAAC TCAATCTCCA GAAAGTGGTA TTCCATACCC AACTTTCTTG TACAAAGTtg
1681gcattataag aaagcattgc ttatcaattt gttgcaacga ac

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