Construct: ORF ccsbBroadEn_08587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013690.1_s300c1, BRDN0000386506
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DEPDC1B (55789)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55789 | DEPDC1B | DEP domain containing 1B | NM_018369.3 | 99.8% | 99.6% | 995G>T;1183G>C |
| 2 | human | 55789 | DEPDC1B | DEP domain containing 1B | XM_011543509.2 | 96.9% | 96.5% | 1_1delAins46;950G>T;1138G>C |
| 3 | human | 55789 | DEPDC1B | DEP domain containing 1B | NM_001145208.2 | 88.1% | 87.9% | 995G>T;1183G>C;1239_1240ins186 |
| 4 | human | 55789 | DEPDC1B | DEP domain containing 1B | XR_948274.3 | 64.5% | (many diffs) | |
| 5 | human | 55789 | DEPDC1B | DEP domain containing 1B | XM_011543510.3 | 61.5% | 57.8% | (many diffs) |
| 6 | human | 55789 | DEPDC1B | DEP domain containing 1B | XM_017009629.2 | 60.6% | 57.4% | (many diffs) |
| 7 | mouse | 218581 | Depdc1b | DEP domain containing 1B | NM_178683.4 | 86.3% | 93.5% | (many diffs) |
| 8 | mouse | 218581 | Depdc1b | DEP domain containing 1B | XM_006517612.3 | 81.9% | 88.8% | (many diffs) |
| 9 | mouse | 218581 | Depdc1b | DEP domain containing 1B | XM_006517613.3 | 71.5% | 78% | (many diffs) |
| 10 | mouse | 218581 | Depdc1b | DEP domain containing 1B | XM_006517614.3 | 62.4% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAGAAAG 61 TTGGCATGGA GCATCGCATC GTGGGGCCCG GGCCGTACCG AGCTACCAGG CTGTGGAATG 121 AGACCGTGGA GCTTTTTCGT GCTAAGATGC CGTTACGGAA ACATCGCTGT CGTTTCAAGA 181 GCTATGAGCA TTGTTTCACA GCGGCCGAAG CTGTGGATTG GCTGCATGAG CTGCTGAGGT 241 GCAGTCAAAA CTTCGGCCCT GAAGTGACCC GCAAACAAAC GGTCCAGCTG CTAAAAAAAT 301 TCCTGAAGAA TCACGTTATT GAAGACATCA AGGGAAAATG GGGTGAGGAA GATTTTGAAG 361 ACAATCGTCA CTTATACAGA TTTCCTCCTT CTTCACCCCT GAAACCATAT CCAAAGAAGC 421 CCCCAAACCA AAAGGATGTT ATTAAATTTC CAGAATGGAA TGATCTCCCA CCAGGCACTT 481 CACAAGAGAA CATCCCAGTG AGGCCAGTTG TGATGAATTC TGAGATGTGG TACAAGCGTC 541 ACAGTATTGC AATTGGAGAG GTGCCAGCTT GCCGTCTTGT CCACCGCAGA CAGCTGACAG 601 AGGCCAATGT AGAAGAGATA TGGAAGTCTA TGACATTATC ATACTTACAG AAAATTCTTG 661 GCCTGGATTC CTTAGAAGAA GTTTTAGACG TCAAACTTGT CAATTCGAAG TTCATCATCC 721 ATAATGTATA TAGTGTTAGC AAGCAGGGAG TTGTTATTCT TGATGACAAG TCAAAAGAAC 781 TTCCTCATTG GGTGCTGTCA GCTATGAAGT GTTTGGCAAA TTGGCCCAAC TGTTCTGATT 841 TGAAGCAGCC TATGTACTTG GGATTTGAAA AAGATGTCTT TAAAACCATA GCTGATTACT 901 ATGGTCACTT GAAAGAGCCT CTACTTACAT TTCATCTTTT TGATGCTTTT GTCAGTGTAC 961 TGGGTTTGTT ACAGAAGGAG AAAGTGGCAG TTGAAGCATT TCAGATTTGC TGCCTTCTCC 1021 TACCTCCTGA AAATAGGAGA AAGTTACAGC TATTGATGAT GATGATGGCA AGGATTTGCT 1081 TAAACAAAGA GATGCCACCC CTGTGTGATG GCTTTGGTAC CCGAACACTG ATGGTTCAGA 1141 CATTTTCCCG TTGCATCTTG TGTTCCAAGG ATGAAGTGGA CTTGGATGAG TTATTAGCTG 1201 CTAGATTGGT AACGTTTCTG ATGGACAATT ACCAGGAAAT TCTGAAACTC CCTTTGGCCT 1261 TGCAGACCTC TATAGAGGAG CGTGTGGCTC ATCTACGAAG AGTCCAGATA AAATACCCAG 1321 GAGCTGATAT GGATATCACT TTATCTGCTC CATCATTTTG CCGTCAAATT AGTCCAGAGG 1381 AATTTGAATA TCAAAGATCA TATGGCTCTC AGGAACCTCT GGCAGCCTTG TTGGAGGAAG 1441 TCATAACAGA TGCCAAACTC TCCAACAAAG AGAAAAAGAA GAAACTGAAG CAGTTTCAGA 1501 AATCCTATCC TGAAGTCTAT CAAGAACGAT TTCCTACACC AGAAAGTGCA GCACTTCTGT 1561 TTCCTGAAAA ACCCAAACCG AAACCACAGC TGCTAATGTG GGCACTAAAG AAGCCTTTCC 1621 AACCATTTCA AAGAACTAGA AGTTTTCGAA TGTACCCAAC TTTTTTGCAC AAAGTtggca 1681 ttataagaaa gcattgctta tcaatttgtt gcaacgaac