Construct: ORF ccsbBroadEn_09222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013151.1_s300c1, BRDN0000386364
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA1C (84790)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_032704.5 | 99.6% | 100% | (many diffs) |
| 2 | human | 10376 | TUBA1B | tubulin alpha 1b | NM_006082.3 | 97% | 98% | (many diffs) |
| 3 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270399.1 | 96.1% | 98% | (many diffs) |
| 4 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_006009.4 | 96.1% | 98% | (many diffs) |
| 5 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303115.2 | 91.8% | 92.2% | (many diffs) |
| 6 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303116.2 | 91.8% | 92.2% | (many diffs) |
| 7 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303117.2 | 91.8% | 92.2% | (many diffs) |
| 8 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270400.1 | 88.4% | 90.2% | (many diffs) |
| 9 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303114.1 | 86.1% | 86.3% | (many diffs) |
| 10 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_006000.3 | 85.1% | 95.9% | (many diffs) |
| 11 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 83.8% | 96.2% | (many diffs) |
| 12 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 83.3% | 96.2% | (many diffs) |
| 13 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 82.9% | 94.8% | (many diffs) |
| 14 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_001278552.1 | 82.2% | 92.8% | (many diffs) |
| 15 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_018943.3 | 80.7% | 89.7% | (many diffs) |
| 16 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_001193414.2 | 69.4% | 78.6% | (many diffs) |
| 17 | human | 7277 | TUBA4A | tubulin alpha 4a | XM_017004824.1 | 56.2% | 63.9% | (many diffs) |
| 18 | mouse | 22146 | Tuba1c | tubulin, alpha 1C | NM_009448.4 | 93.9% | 99.3% | (many diffs) |
| 19 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | NM_011654.2 | 92.7% | 98% | (many diffs) |
| 20 | mouse | 22142 | Tuba1a | tubulin, alpha 1A | NM_011653.2 | 91.8% | 98% | (many diffs) |
| 21 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | XM_006520756.1 | 84.5% | 89.2% | (many diffs) |
| 22 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_009447.4 | 84.1% | 95.9% | (many diffs) |
| 23 | mouse | 22147 | Tuba3b | tubulin, alpha 3B | NM_009449.3 | 84% | 96.2% | (many diffs) |
| 24 | mouse | 22144 | Tuba3a | tubulin, alpha 3A | NM_009446.3 | 83.1% | 96.2% | (many diffs) |
| 25 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | XM_006496478.1 | 81.4% | 92.8% | (many diffs) |
| 26 | mouse | 53857 | Tuba8 | tubulin, alpha 8 | NM_017379.2 | 78.8% | 89.9% | (many diffs) |
| 27 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313724.1 | 55.8% | 63.9% | (many diffs) |
| 28 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313723.1 | 46.4% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 1414
- ORF length:
- 1347
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG NTACAAAAAA 61 GTTGGCATGC GTGAGTGCAT CTCCATCCAC GTTGGCCAGG CTGGTGTCCA GATTGGCAAT 121 GCCTGCTGGG AGCTCTACTG CCTGGAACAC GGCATCCAGC CCGATGGCCA GATGCCAAGT 181 GACAAGACCA TTGGGGGAGG AGATGATTCC TTCAACACCT TCTTCAGTGA AACGGGTGCT 241 GGCAAGCATG TGCCCCGGGC AGTGTTTGTA GACTTGGAAC CCACAGTCAT TGATGAAGTT 301 CGCACTGGCA CCTACCGCCA GCTCTTCCAC CCTGAGCAGC TCATCACAGG CAAGGAAGAT 361 GCTGCCAATA ACTATGCCCG AGGGCACTAC ACCATTGGCA AGGAGATCAT TGACCTCGTG 421 TTGGACCGAA TTCGCAAGCT GGCTGACCAG TGCACCGGTC TTCAGGGCTT CTTGGTTTTC 481 CACAGCTTTG GTGGGGGAAC TGGTTCTGGG TTCACCTCGC TGCTCATGGA ACGTCTCTCA 541 GTTGATTATG GCAAGAAGTC CAAGCTGGAG TTCTCTATTT ACCCGGCGCC CCAGGTTTCC 601 ACAGCTGTAG TTGAGCCCTA CAACTCCATC CTCACCACCC ACACCACCCT GGAGCACTCT 661 GATTGTGCCT TCATGGTAGA CAATGAGGCC ATCTATGACA TCTGTCGTAG AAACCTCGAT 721 ATCGAGCGCC CAACCTACAC TAACCTTAAC CGCCTTATTA GCCAGATTGT GTCCTCCATC 781 ACTGCTTCCC TGAGATTTGA TGGAGCCCTG AATGTTGACC TGACAGAATT CCAGACCAAC 841 CTGGTGCCCT ACCCCCGCAT CCACTTCCCT CTGGCCACAT ATGCCCCTGT CATCTCTGCT 901 GAGAAAGCCT ACCATGAACA GCTTACTGTA GCAGAGATCA CCAATGCTTG CTTTGAGCCA 961 GCCAACCAGA TGGTGAAATG TGACCCTCGC CATGGTAAAT ACATGGCTTG CTGCCTGTTA 1021 TACCGTGGTG ACGTGGTTCC CAAAGATGTC AATGCTGCCA TTGCCACCAT CAAAACCAAG 1081 CGTACCATCC AGTTTGTGGA TTGGTGCCCC ACTGGCTTCA AGGTTGGCAT TAATTACCAG 1141 CCTCCCACTG TGGTGCCTGG CGGAGACCTG GCCAAGGTAC AGAGAGCTGT GTGCATGCTG 1201 AGCAATACCA CAGCTGTTGC TGAGGCCTGG GCTCGCCTGG ACCACAAGTT TGACCTGATG 1261 TATGCCAAGC GTGCCTTTGT TCACTGGTAC GTGGGTGAGG GGATGGAGGA AGGCGAGTTT 1321 TCAGAGGCCC GTGAGGACAT GGCTGCCCTT GAGAAGGATT ATGAGGAGGT TGGAGCAGAT 1381 AGTGCTGACG GAGAGGATGA GGGTGAAGAG TATTACCCAA CTTTCTTGTA CAAAGTtggc 1441 attataagaa agcattgctt atcaatttgt tgcaacgaac