Transcript: Human NM_001303115.2

Homo sapiens tubulin alpha 1c (TUBA1C), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
TUBA1C (84790)
Length:
3101
CDS:
484..1728

Additional Resources:

NCBI RefSeq record:
NM_001303115.2
NBCI Gene record:
TUBA1C (84790)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001303115.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000248507 TGACCCTCGCCATGGTAAATA pLKO_005 1293 CDS 100% 15.000 7.500 Y Tuba1c n/a
2 TRCN0000029141 GCTGCCCTTGAGAAGGATTAT pLKO.1 1654 CDS 100% 13.200 6.600 Y TUBA1C n/a
3 TRCN0000157025 GCTGCCCTTGAGAAGGATTAT pLKO.1 1654 CDS 100% 13.200 6.600 Y TUBA1B n/a
4 TRCN0000319306 GCTGCCCTTGAGAAGGATTAT pLKO_005 1654 CDS 100% 13.200 6.600 Y TUBA1C n/a
5 TRCN0000248506 CCATTGGCAAGGAGATCATTG pLKO_005 704 CDS 100% 10.800 5.400 Y Tuba1c n/a
6 TRCN0000029139 CCCAACCTACACTAACCTTAA pLKO.1 1041 CDS 100% 10.800 5.400 Y TUBA1C n/a
7 TRCN0000319305 CCCAACCTACACTAACCTTAA pLKO_005 1041 CDS 100% 10.800 5.400 Y TUBA1C n/a
8 TRCN0000029140 GCAAGGAAGATGCTGCCAATA pLKO.1 662 CDS 100% 10.800 5.400 Y TUBA1C n/a
9 TRCN0000349678 GCAAGGAAGATGCTGCCAATA pLKO_005 662 CDS 100% 10.800 5.400 Y TUBA1C n/a
10 TRCN0000248508 TAGACAATGAGGCCATCTATG pLKO_005 989 CDS 100% 10.800 5.400 Y Tuba1c n/a
11 TRCN0000153763 CCACAAGTTTGACCTGATGTA pLKO.1 1554 CDS 100% 4.950 2.475 Y TUBA1B n/a
12 TRCN0000319311 CCACAAGTTTGACCTGATGTA pLKO_005 1554 CDS 100% 4.950 2.475 Y TUBA1B n/a
13 TRCN0000029142 CCCACAGTCATTGATGAAGTT pLKO.1 592 CDS 100% 4.950 2.475 Y TUBA1C n/a
14 TRCN0000153865 CCCACAGTCATTGATGAAGTT pLKO.1 592 CDS 100% 4.950 2.475 Y TUBA1B n/a
15 TRCN0000319375 CCCACAGTCATTGATGAAGTT pLKO_005 592 CDS 100% 4.950 2.475 Y TUBA1C n/a
16 TRCN0000123592 CCTGTCATCTCTGCTGAGAAA pLKO.1 1198 CDS 100% 4.950 2.475 Y Tuba1a n/a
17 TRCN0000029143 GCACTCTGATTGTGCCTTCAT pLKO.1 966 CDS 100% 4.950 2.475 Y TUBA1C n/a
18 TRCN0000178352 GTGGTTCCCAAAGATGTCAAT pLKO.1 1345 CDS 100% 4.950 2.475 Y Tuba1c n/a
19 TRCN0000091395 CTACACCATTGGCAAGGAGAT pLKO.1 699 CDS 100% 4.050 2.025 Y LOC434390 n/a
20 TRCN0000152522 GAGAAGGATTATGAGGAGGTT pLKO.1 1663 CDS 100% 2.640 1.320 Y TUBA1B n/a
21 TRCN0000181722 GATGCCAAGTGACAAGACCAT pLKO.1 483 5UTR 100% 2.640 1.320 Y Tuba1b n/a
22 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2890 3UTR 100% 5.625 2.813 Y KLHL30 n/a
23 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2890 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001303115.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16042 pDONR223 0% 92.2% 92.2% None 0_1ins105 n/a
2 ccsbBroad304_16042 pLX_304 0% 92.2% 92.2% V5 0_1ins105 n/a
3 ccsbBroadEn_16043 pDONR223 0% 92.1% 92.2% None 0_1ins105;744C>T n/a
4 ccsbBroad304_16043 pLX_304 0% 92.1% 92.2% V5 0_1ins105;744C>T n/a
5 TRCN0000465274 AATTCTCGGGCCTTAAGCACTAAC pLX_317 23.4% 92.1% 92.2% V5 0_1ins105;744C>T n/a
6 ccsbBroadEn_09222 pDONR223 100% 91.8% 92.2% None (many diffs) n/a
7 ccsbBroad304_09222 pLX_304 0% 91.8% 92.2% V5 (many diffs) n/a
8 ccsbBroadEn_15707 pDONR223 0% 89.1% 90.2% None (many diffs) n/a
9 ccsbBroad304_15707 pLX_304 0% 89.1% 90.2% V5 (many diffs) n/a
10 TRCN0000467476 CACCGTCGAGGCCGCTGTATAAGA pLX_317 31.7% 89.1% 90.2% V5 (many diffs) n/a
11 ccsbBroadEn_15706 pDONR223 0% 89% 90.2% None (many diffs) n/a
12 ccsbBroad304_15706 pLX_304 0% 89% 90.2% V5 (many diffs) n/a
13 TRCN0000472335 TTCTGAAATAAGACACGCCGGGCA pLX_317 37.9% 89% 90.2% V5 (many diffs) n/a
14 ccsbBroadEn_02413 pDONR223 100% 89% 90.2% None (many diffs) n/a
15 ccsbBroad304_02413 pLX_304 0% 89% 90.2% V5 (many diffs) n/a
16 TRCN0000466437 GGCACCAGGAAACTAGGGGTGTGT pLX_317 31.7% 89% 90.2% V5 (many diffs) n/a
17 ccsbBroadEn_01831 pDONR223 100% 88.1% 90.2% None (many diffs) n/a
18 ccsbBroad304_01831 pLX_304 0% 88.1% 90.2% V5 (many diffs) n/a
19 TRCN0000466264 GACTTCAAATAAATAGCTTCACTC pLX_317 23.8% 88.1% 90.2% V5 (many diffs) n/a
20 ccsbBroadEn_07108 pDONR223 100% 78.6% 88.6% None (many diffs) n/a
21 ccsbBroad304_07108 pLX_304 0% 78.6% 88.6% V5 (many diffs) n/a
22 TRCN0000467865 AGTTCGTGAAACATACGCCGGCAG pLX_317 34.6% 78.6% 88.6% V5 (many diffs) n/a
23 ccsbBroadEn_09386 pDONR223 100% 76.5% 88.2% None (many diffs) n/a
24 ccsbBroad304_09386 pLX_304 0% 76.5% 88.2% V5 (many diffs) n/a
25 TRCN0000465924 GTCATGGAGAAAGTCGGTGACCGG pLX_317 28.5% 76.5% 88.2% V5 (many diffs) n/a
26 ccsbBroadEn_09375 pDONR223 100% 76.2% 87.3% None (many diffs) n/a
27 ccsbBroad304_09375 pLX_304 0% 76.2% 87.3% V5 (many diffs) n/a
28 TRCN0000469142 GAGGCCACAGGCCATAAACTAATT pLX_317 29% 76.2% 87.3% V5 (many diffs) n/a
29 ccsbBroadEn_15708 pDONR223 0% 63.6% 64.5% None (many diffs) n/a
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