Construct: ORF ccsbBroadEn_12180

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010082.1_s300c1, BRDN0000456492
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RIC8B (55188)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001351364.2 100% 100%
2 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001330147.2 92.1% 91.7% (many diffs)
3 human 55188 RIC8B RIC8 guanine nucleotide exc... XR_002957346.1 90.2% (many diffs)
4 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001330146.2 89.4% 88.9% (many diffs)
5 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001351361.2 88.3% 87.6% (many diffs)
6 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001351363.2 87.7% 85.9% (many diffs)
7 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001330145.2 86.9% 86.4% (many diffs)
8 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001351362.2 86.6% 84.6% (many diffs)
9 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_018157.4 85.2% 83.4% (many diffs)
10 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001351366.2 84.7% 84.6% (many diffs)
11 human 55188 RIC8B RIC8 guanine nucleotide exc... NM_001351367.2 82.8% 81.2% (many diffs)
12 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147135.2 28.8% (many diffs)
13 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147137.2 28.2% (many diffs)
14 human 55188 RIC8B RIC8 guanine nucleotide exc... XR_002957348.1 28% (many diffs)
15 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147138.2 27.4% (many diffs)
16 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147133.2 27.3% (many diffs)
17 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147131.2 27.2% (many diffs)
18 human 55188 RIC8B RIC8 guanine nucleotide exc... XR_001748786.2 26.3% (many diffs)
19 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147139.2 26.3% (many diffs)
20 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147136.2 26.1% (many diffs)
21 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147132.2 25.4% (many diffs)
22 human 55188 RIC8B RIC8 guanine nucleotide exc... NR_147134.2 24.7% (many diffs)
23 human 55188 RIC8B RIC8 guanine nucleotide exc... XR_001748785.2 14.9% (many diffs)
24 human 55188 RIC8B RIC8 guanine nucleotide exc... XR_002957347.1 14.9% (many diffs)
25 human 55188 RIC8B RIC8 guanine nucleotide exc... XR_002957345.1 13.2% 0_1insATGCTTGCCAGT;1477_11092del
26 mouse 237422 Ric8b RIC8 guanine nucleotide exc... XM_006513625.2 87% 89.3% (many diffs)
27 mouse 237422 Ric8b RIC8 guanine nucleotide exc... NM_183172.2 82.5% 84.1% (many diffs)
28 mouse 237422 Ric8b RIC8 guanine nucleotide exc... NM_001013441.2 79.8% 81% (many diffs)
29 mouse 237422 Ric8b RIC8 guanine nucleotide exc... XR_380393.2 60.6% (many diffs)
30 mouse 237422 Ric8b RIC8 guanine nucleotide exc... XR_380394.3 24.6% (many diffs)
31 mouse 237422 Ric8b RIC8 guanine nucleotide exc... XR_380392.2 24.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1557
ORF length:
1488
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCTTGCCAGT AAACTCTGTG AAGGCATATT TAAAGTCCTT ATAAAGGACA
121TCCCAACAAC ATGTCAAGTG TCCTGCCTGG AAGTACTCCG CATTCTCTCC AGAGACAAAA
181AGGTTTTAGT TCCTGTGACA ACTAAGGAAA ATATGCAGAT ACTGCTGCGA CTAGCCAAGC
241TAAATGAGTT AGATGATTCT TTGGAGAAAG TATCAGAGTT CCCAGTTATT GTGGAGTCAT
301TAAAATGTCT GTGTAATATA GTGTTCAACA GTCAGATGGC ACAGCAGCTC AGCCTGGAAC
361TTAATCTTGC TGCAAAGCTC TGTAACCTCC TGAGAAAGTG CAAGGACCGG AAATTTATCA
421ATGACATTAA GTGCTTTGAC TTGCGCTTGC TCTTCCTTCT GTCACTTTTG CACACCGACA
481TCAGGTCACA ATTGCGCTAT GAGCTCCAGG GACTACCGCT GCTAACGCAG ATCTTGGAAA
541GTGCCTTTAG CATCAAGTGG ACCGATGAGT ATGAATCGGC CATAGACCAT AATGGACCTC
601CTCTCTCACC TCAGGAGACA GACTGTGCCA TTGAGGCCCT CAAAGCTCTC TTCAATGTGA
661CGGTAGACAG TTGGAAGGTG CATAAAGAGA GTGATTCTCA TCAGTTCCGT GTAATGGCAG
721CTGTCCTTCG TCATTGTTTA CTAATCGTAG GTCCAACTGA AGACAAAACA GAAGAGCTAC
781ACAGCAATGC AGTCAACCTT TTAAGCAATG TTCCAGTCTC TTGTTTGGAT GTTCTCATTT
841GTCCGTTAAC CCATGAAGAA ACAGCCCAAG AGGCAACGAC TCTAGATGAA CTGCCCAGTA
901ATAAAACAGC TGAGAAAGAA ACAGTTTTGA AAAACAATAC CATGGTATAC AATGGTATGA
961ATATGGAGGC CATTCATGTT TTACTGAATT TTATGGAGAA GAGAATAGAC AAGGGAAGCA
1021GCTATAGAGA GGGTCTAACT CCAGTTCTCA GCTTATTAAC CGAATGTTCC CGAGCCCATC
1081GAAACATCCG AAAATTTCTC AAAGATCAGG TTTTACCACC GTTGAGGGAT GTGACAAATC
1141GACCTGAAGT TGGCTCAACT GTGAGAAATA AGCTGGTGCG CCTCATGACA CATGTTGACC
1201TTGGAGTCAA GCAAATTGCT GCTGAATTCC TTTTTGTCCT TTGCAAAGAG AGAGTGGATA
1261GTCTGCTGAA ATACACTGGC TATGGGAATG CTGCAGGACT GTTGGCGGCC AGGGGCCTCT
1321TGGCTGGAGG AAGAGGAGAT AATTGGTACT CAGAGGATGA GGACACAGAC ACTGAAGAAT
1381ACAAAAATGC AAAACCAAAC ATTAATCTTA TCACTGGTCA TTTAGAGGAA CCAATGCCAA
1441ACCCCATAGA TGAAATGACA GAAGAACAAA AAGAATATGA AGCCATGAAA CTTGTCAACA
1501TGCTTGATAA ACTTTCCAGG AGAGCAGATG TGAAGGACCT GCACCAGGAT GGTGGTTTGC
1561CAACTTTCTT GTACAAAGTT GGcattataa gaaagcattg cttatcaatt tgttgcaacg
1621aac

Download FASTA (ORF) (Full)