Transcript: Human NM_001330146.2

Homo sapiens RIC8 guanine nucleotide exchange factor B (RIC8B), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
RIC8B (55188)
Length:
5000
CDS:
65..1699

Additional Resources:

NCBI RefSeq record:
NM_001330146.2
NBCI Gene record:
RIC8B (55188)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330146.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230248 CTGCGACTAGCCAAGCTAAAT pLKO_005 293 CDS 100% 13.200 18.480 N RIC8B n/a
2 TRCN0000230249 GAATCGGCCATAGACCATAAT pLKO_005 641 CDS 100% 13.200 18.480 N RIC8B n/a
3 TRCN0000230250 TTCTCATCAGTTCCGTGTAAT pLKO_005 763 CDS 100% 13.200 18.480 N RIC8B n/a
4 TRCN0000219033 GATGAACTGCCCAGTAATAAA pLKO_005 953 CDS 100% 15.000 10.500 N RIC8B n/a
5 TRCN0000257144 TTAAATCCAGAACGCTTTATA pLKO_005 3558 3UTR 100% 15.000 10.500 N RIC8B n/a
6 TRCN0000006464 CCAGTTATTGTGGAGTCATTA pLKO.1 350 CDS 100% 13.200 9.240 N RIC8B n/a
7 TRCN0000436180 GCTTAAACCAATGGGACTAAA pLKO_005 1597 CDS 100% 13.200 9.240 N Ric8b n/a
8 TRCN0000420175 CATTAACTGGTTTACTCATTG pLKO_005 1808 3UTR 100% 10.800 7.560 N Ric8b n/a
9 TRCN0000006463 CCTCAGACTTACAAACTGATT pLKO.1 2247 3UTR 100% 4.950 3.465 N RIC8B n/a
10 TRCN0000006466 GCTCTCTTCAATGTGACGGTA pLKO.1 713 CDS 100% 2.640 1.848 N RIC8B n/a
11 TRCN0000418668 ACTTGTCAACATGCTTGATAA pLKO_005 1558 CDS 100% 13.200 7.920 N Ric8b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330146.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12180 pDONR223 100% 89.4% 88.9% None (many diffs) n/a
2 ccsbBroad304_12180 pLX_304 0% 89.4% 88.9% V5 (many diffs) n/a
3 TRCN0000477167 ACGTCCTACCCTAAGCCCTGGAGT pLX_317 19.1% 89.4% 88.9% V5 (many diffs) n/a
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