Construct: ORF ccsbBroadEn_14950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018975.1_s300c1, BRDN0000458271
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT3 (10000)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001370074.1 | 99% | 97.5% | (many diffs) |
| 2 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_005465.7 | 99% | 97.5% | (many diffs) |
| 3 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001206729.1 | 94.9% | 91.8% | (many diffs) |
| 4 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_181690.2 | 94.9% | 91.8% | (many diffs) |
| 5 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446000.1 | 88.5% | 86.4% | (many diffs) |
| 6 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_016999985.1 | 86.4% | 85% | (many diffs) |
| 7 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446892.1 | 59.5% | 55.6% | (many diffs) |
| 8 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_011544014.2 | 50% | 44.6% | (many diffs) |
| 9 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024447938.1 | 49.8% | 47.7% | (many diffs) |
| 10 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | NM_011785.3 | 93.1% | 97% | (many diffs) |
| 11 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496818.3 | 93.1% | 97% | (many diffs) |
| 12 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496819.3 | 93.1% | 97% | (many diffs) |
| 13 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_011238805.2 | 83.5% | 83.2% | (many diffs) |
| 14 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_017320418.1 | 80.9% | 84.5% | (many diffs) |
| 15 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496821.3 | 53.7% | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 1535
- ORF length:
- 1440
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCATGAGA GATGTTACCA TTGTGAAAGA 121 AGGTTGGGTT CAGAAGAGGG GAGAATATAT AAAAAACTGG AGGCCAAGAT ACTTCCTTTT 181 GAAGACAGAT GGCTCATTCA TAGGATATAA AGAGAAACCT CAAGATGTGG ATTTACCTTA 241 TCCCCTCAAC AACTTTTCAG TGGCAAAATG CCAGTTAATG AAAACAGAAC GACCAAAGCC 301 AAACACATTT ATAATCAGAT GTCTCCAGTG GACTACTGTT ATAGAGAGAA CATTTCATGT 361 AGATACTCCA GAGGAAAGGG AAGAATGGAC AGAAGCTATC CAGGCTGTAG CAGACAGACT 421 GCAGAGGCAA GAAGAGGAGA GAATGAATTG TAGTCCAACT TCACAAATTG ATAATATAGG 481 AGAGGAAGAG ATGGATGCCT CTACAACCCA TCATAAAAGA AAGACAATGA ATGATTTTGA 541 CTATTTGAAA CTACTAGGTA AAGGCACTTT TGGGAAAGTT ATTTTGGTTC GAGAGAAGGC 601 AAGTGGAAAA TACTATGCTA TGAAGATTCT GAAGAAAGAA GTCATTATTG CAAAGGATGA 661 AGTGGCACAC ACTCTAACTG AAAGCAGAGT ATTAAAGAAC ACTAGACATC CCTTTTTAAC 721 ATCCTTGAAN TATNCNTNCC AGANAAAAGA CNGTTTGTGT TTTGTGATGG AATATGTTAN 781 NGGGGGCGAG CTGTTTTTCC ATTTGTCGAG AGAGCGGGTG TTCTCTGAGG ACCGCACACG 841 TTTCTATGGT GCAGAAATTG TCTCTGCCTT GGACTATCTA CATTCCCGGN AAGATTTGTG 901 GTACCGTGAT CTCAAGTTGG AGAATCTAAT GCTGGACAAA GATGGCCACA TAAAAATTAC 961 AGATTTTGGA CTTTGCAAAG AAGGGATCAC AGATGCAGCC ACCATGAAGA CATTCTGTGG 1021 CACTCCAGAA TATCTGGCAC CAGAGGTGTT AGAAGATAAT GACTATGGCC GAGCAGTAGA 1081 CTGGTGGGGC CTAGGGGTTG TCATGTATGA AATGATGTGT GGGAGGTTAC CTTTCTACAA 1141 CCAGGACCAT GAGAAACTTT TTGAATTAAT ATTAATGGAA GACATTAAAT TTCCTCGAAC 1201 ACTCTCTTCA GATGCAAAAT CATTGCTTTC AGGGCTCTTG ATAAAGGATC CAAATAAACG 1261 CCTTGGTGGA GGACCAGATG ATGCAAAAGA AATTATGAGA CACAGTTTCT TCTCTGGAGT 1321 AAACTGGCAA GATGTATATG ATAAAAAGCT TGTACCTCCT TTTAAACCTC AAGTAACATC 1381 TGAGACAGAT ACTAGATATT TTGATGAAGA ATTTACAGCT CAGACTATTA CAATAACACC 1441 ACCTGAAAAA TATGATGAGG ATGGTATGGA CTGCATGGAC AATGAGAGGC GGCCGCATTT 1501 CCCTCAATTT TCCTACTCTG CAAGTGGACG AGAATTGCCA ACTTTCTTGT ACAAAGTTGG 1561 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 1621 TAAAATCATT ATTGCCATCC AGCTCATACC CCCCCA