Construct: ORF ccsbBroadEn_15341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018789.1_s300c1, BRDN0000458085
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NME1-NME2 (654364)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 99.5% | 8.2% | (many diffs) |
| 2 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 62.7% | (many diffs) | |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 56.6% | 13.8% | 0_1ins346;386A>N;445_446insG |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 56.6% | 13.8% | 0_1ins346;386A>N;445_446insG |
| 5 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 56.6% | 13.8% | 0_1ins346;386A>N;445_446insG |
| 6 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 56.6% | 13.8% | 0_1ins346;386A>N;445_446insG |
| 7 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 53.4% | 14.4% | (many diffs) |
| 8 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 48.8% | 12.4% | (many diffs) |
| 9 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001198682.2 | 30.6% | 25.6% | 0_1ins346;227_228ins113;246_247ins98 |
| 10 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_001077529.2 | 51.1% | 13.8% | (many diffs) |
| 11 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_008705.5 | 51.1% | 13.8% | (many diffs) |
| 12 | mouse | 433968 | Gm5566 | predicted pseudogene 5566 | XR_868740.1 | 45.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 183
- ORF length:
- 84
- Sequence:
-
1 GATGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GGCCAACTGT GAGCGTACCT 121 TCATTGCGAT CAANCCAGAT GGGGTCCAGC GGGGTCTTGT GGGGAGAGAT TATCAAGCGT 181 TTTGAGCAGA AAGGATTCCG CCTTGTTGGT CTGAAATTCA TGCAAGCTTC CGAAGATCTT 241 CTCAAGGAAC ACTACGTTGA CCTGAAGGAC CGTCCATTCT TTGCCGGCCT GGTGAAATAC 301 ATGCACTCAG GGCCGGTAGT TGCCATGGTC TGGGAGGGGC TGAATGTGGT GAAGACGGGC 361 CGAGTCATGC TCGGGGAGAC CAACCCTGCA GACTCCAAGC CTGGGACCAT CCGTGGAGAC 421 TTCTGCATAC AAGTTGGCAG GACCATGGCC AACCTGGAGC GCACCTTCAT CGCCATCAAG 481 CCGGACGGCG TGCAGCGCGG CCTGGTGGGC GAGATCATCA AGCGCTTCGA GCAGAAGGGA 541 TTCCGCCTCG TGGCCATGAA GTTCCTCCGG GCCTCTGAAG AACACCTGAA GCAGCACTAC 601 ATTGACCTGA AAGACCGACC ATTCTTCCCT GGGCTGGTGA AGTACATGAA CTCAGGGCCG 661 GTTGTGGCCA TGGTCTGGGA GGGGCTGAAC GTGGTGAAGA CAGGCCGAGT GATGCTTGGG 721 GAGACCAATC CAGCAGATTC AAAGCCAGGC ACCATTCGTG GGGACTTCTG CATTCAGGTT 781 GGCAGGAACA TCATTCATGG CAGTGATTCA GTAAAAAGTG CTGAAAAAGN AATCAGCCTA 841 TGGTTTAAGC CTGAAGAACT GGTTGACTAC AAGTCTTGTG CTCATGACTG GGGTCTATGA 901 ATTGCCAACT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 961 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT GCCATCCAGC TCTTC