Construct: ORF ccsbBroadEn_15708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009898.1_s300c1, BRDN0000385886
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA1B (10376)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10376 | TUBA1B | tubulin alpha 1b | NM_006082.3 | 74.2% | 74.2% | 321_668del |
| 2 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270399.1 | 72.8% | 73.8% | (many diffs) |
| 3 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_006009.4 | 72.8% | 73.8% | (many diffs) |
| 4 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_032704.5 | 71.3% | 72.2% | (many diffs) |
| 5 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270400.1 | 65% | 66% | (many diffs) |
| 6 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_001193414.2 | 63.6% | 54.7% | (many diffs) |
| 7 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303115.2 | 63.6% | 64.5% | (many diffs) |
| 8 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303116.2 | 63.6% | 64.5% | (many diffs) |
| 9 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303117.2 | 63.6% | 64.5% | (many diffs) |
| 10 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 63.5% | 72.5% | (many diffs) |
| 11 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 63.4% | 72.5% | (many diffs) |
| 12 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_006000.3 | 63.3% | 70.9% | (many diffs) |
| 13 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 63.1% | 71.3% | (many diffs) |
| 14 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303114.1 | 61.7% | 62.3% | (many diffs) |
| 15 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_001278552.1 | 60.4% | 67.8% | (many diffs) |
| 16 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_018943.3 | 60.2% | 65.8% | (many diffs) |
| 17 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | NM_011654.2 | 70.2% | 74.2% | (many diffs) |
| 18 | mouse | 22142 | Tuba1a | tubulin, alpha 1A | NM_011653.2 | 69.9% | 73.8% | (many diffs) |
| 19 | mouse | 22146 | Tuba1c | tubulin, alpha 1C | NM_009448.4 | 69.2% | 72.5% | (many diffs) |
| 20 | mouse | 22147 | Tuba3b | tubulin, alpha 3B | NM_009449.3 | 64.1% | 72.5% | (many diffs) |
| 21 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | XM_006520756.1 | 63.9% | 67.6% | (many diffs) |
| 22 | mouse | 22144 | Tuba3a | tubulin, alpha 3A | NM_009446.3 | 63.1% | 72.5% | (many diffs) |
| 23 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_009447.4 | 63% | 70.9% | (many diffs) |
| 24 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313723.1 | 61.8% | 64.2% | (many diffs) |
| 25 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | XM_006496478.1 | 60.3% | 67.8% | (many diffs) |
| 26 | mouse | 53857 | Tuba8 | tubulin, alpha 8 | NM_017379.2 | 59.2% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgcg tgagtgcatc tccatccacg ttggccaggc tggtgtccag attggcaatg 121 cctgctggga gctctactgc ctggaacacg gcatccagcc cgatggccag atgccaagtg 181 acaagaccat tgggggagga gatgactcct tcaacacctt cttcagtgag acgggcgctg 241 gcaagcacgt gccccgggct gtgtttgtag acttggaacc cacagtcatt gatgaagttc 301 gcactggcac ctaccgccag ctcttccacc ctgagcagct catcacaggc aaggaagatg 361 ctgccaataa ctatgcccga gggcactaca ctaaccttaa ccgccttatt agccagattg 421 tgtcctccat cactgcttcc ctgagatttg atggagccct gaatgttgac ctgacagaat 481 tccagaccaa cctggtgccc tacccccgca tccacttccc tctggccaca tatgcccctg 541 tcatctctgc tgagaaagcc taccatgaac agctttctgt agcagagatc accaatgctt 601 gctttgagcc agccaaccag atggtgaaat gtgaccctcg ccatggtaaa tacatggctt 661 gctgcctgtt gtaccgtggt gacgtggttc ccaaagatgt caatgctgcc attgccacca 721 tcaaaaccaa gcgcagcatc cagtttgtgg attggtgccc cactggcttc aaggttggca 781 tcaactacca gcctcccact gtggtgcctg gtggagacct ggccaaggta cagagagctg 841 tgtgcatgct gagcaacacc acagccattg ctgaggcctg ggctcgcctg gaccacaagt 901 ttgacctgat gtatgccaag cgtgcctttg ttcactggta cgtgggtgag gggatggagg 961 aaggcgagtt ttcagaggcc cgtgaagata tggctgccct tgagaaggat tatgaggagg 1021 ttggtgtgga ttctgttgaa ggagagggtg aggaagaagg agaggaatac tnnccaactt 1081 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaac