Construct: ORF TRCN0000465604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006665.1_s317c1
- Derived from:
- ccsbBroadEn_13866
- DNA Barcode:
- TAACCACCCTTTTATTTACAACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTM4 (2948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NM_000850.5 | 99.2% | 98.1% | (many diffs) |
| 2 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | NM_000561.4 | 92% | 85.3% | (many diffs) |
| 3 | human | 2946 | GSTM2 | glutathione S-transferase mu 2 | NM_000848.4 | 88.3% | 81.1% | (many diffs) |
| 4 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NM_147148.3 | 87.9% | 85% | (many diffs) |
| 5 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_024446577.1 | 87.2% | 86.2% | (many diffs) |
| 6 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | NM_000851.4 | 85.8% | 82.1% | (many diffs) |
| 7 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | XM_005270784.4 | 85.8% | 82.1% | (many diffs) |
| 8 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001085.1 | 78.2% | 75.3% | (many diffs) |
| 9 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | XM_005270782.5 | 77.4% | 71.1% | (many diffs) |
| 10 | human | 2946 | GSTM2 | glutathione S-transferase mu 2 | NM_001142368.1 | 77% | 71.1% | (many diffs) |
| 11 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001087.2 | 76.3% | 71% | (many diffs) |
| 12 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | NM_146421.3 | 75.7% | 69.2% | (many diffs) |
| 13 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001086.1 | 59.3% | 53% | (many diffs) |
| 14 | human | 442245 | GSTM2P1 | glutathione S-transferase m... | NR_002932.2 | 49.7% | (many diffs) | |
| 15 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NR_024538.1 | 39.3% | (many diffs) | |
| 16 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001088.2 | 38.7% | 35.5% | (many diffs) |
| 17 | mouse | 14865 | Gstm4 | glutathione S-transferase, ... | NM_026764.3 | 85% | 85.3% | (many diffs) |
| 18 | mouse | 14865 | Gstm4 | glutathione S-transferase, ... | NM_001160411.1 | 72.4% | 72% | (many diffs) |
| 19 | mouse | 14865 | Gstm4 | glutathione S-transferase, ... | XM_006501018.2 | 72.4% | 72% | (many diffs) |
| 20 | mouse | 14865 | Gstm4 | glutathione S-transferase, ... | XM_006501020.3 | 72.4% | 72% | (many diffs) |
| 21 | mouse | 14865 | Gstm4 | glutathione S-transferase, ... | XM_006501021.3 | 72.4% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catgccactg ggggactggg acatccgcgg gctggcccac gccatccgcc 121 tgctcctgga atacacagac tcaagctacg aggaaaagaa gtatacgatg ggggacgctc 181 ctgactatga cagaagccag tggctgaatg aaaaattcaa gctgggcctg gactttccca 241 atctgcccta cttgattgat ggggctcaca agatcaccca gagcaacgcc atcctgtgct 301 acattgcccg caagcacaac ctgtgtgggG AGACAGAAGA GGAGAAGATT CGTGTGGACA 361 TTTTGGAGAA CCAGGCTATG GACGTCTCCA ATCAGCTGGC CAGAGTCTGC TACAGCCCTG 421 ACTTTGAGAA ACTGAAGCCA GAATACTTGG AGGAACTTCC TACAATGATG CAGCACTTCT 481 CACAGTTCCT GGGGAAGAGG CCATGGTTTG TTGGAGACAA GATCACCTTT GTAGATTTCC 541 TCGCCTATGA TGTCCTTGAC CTCCACCGTA TATTTGAGCC CAACTGCTTG GACGCCTTCC 601 CAAATCTGAA GGACTTCATC TCCCGCTTTG AGGGCTTGGA GAAGATCTCT GCCTACATGA 661 AGTCCAGCCG CTTCCTCCCA AAACCTCTGT ACACAAGGGT GGCTGTCTGG GGCAGCCAGT 721 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGATAACC ACCCTTTTAT TTACAACGAA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt