Construct: ORF TRCN0000471453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002634.1_s317c1
- Derived from:
- ccsbBroadEn_06334
- DNA Barcode:
- CAGTGATATCGATAATGCGTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTM5 (2949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | NM_000851.4 | 99.8% | 99.5% | 199G>A |
| 2 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | XM_005270784.4 | 99.8% | 99.5% | 199G>A |
| 3 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | NM_000561.4 | 90.6% | 87.1% | (many diffs) |
| 4 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NM_000850.5 | 88.1% | 82.5% | (many diffs) |
| 5 | human | 2946 | GSTM2 | glutathione S-transferase mu 2 | NM_000848.4 | 87.7% | 81.6% | (many diffs) |
| 6 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NM_147148.3 | 80.5% | 74.2% | (many diffs) |
| 7 | human | 2946 | GSTM2 | glutathione S-transferase mu 2 | NM_001142368.1 | 78.7% | 73.3% | (many diffs) |
| 8 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_024446577.1 | 77.4% | 72.5% | (many diffs) |
| 9 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | XM_005270782.5 | 75.8% | 72.4% | (many diffs) |
| 10 | human | 2944 | GSTM1 | glutathione S-transferase mu 1 | NM_146421.3 | 75.5% | 72% | (many diffs) |
| 11 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | XM_017001085.1 | 74.8% | 66.4% | (many diffs) |
| 12 | human | 2949 | GSTM5 | glutathione S-transferase mu 5 | XM_005270785.4 | 52.2% | 52.2% | 0_1ins312 |
| 13 | human | 442245 | GSTM2P1 | glutathione S-transferase m... | NR_002932.2 | 49.4% | (many diffs) | |
| 14 | human | 2948 | GSTM4 | glutathione S-transferase mu 4 | NR_024538.1 | 34.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc catgactctg gggtactggg acatccgtgg gctggcccac gccatccgct 121 tgctcctgga atacacagac tcaagctatg tggaaaagaa gtacacgctg ggggacgctc 181 ctgactatga cagaagccag tggctgaatg aaaaattcaa gctgggcctg gactttccca 241 atctgcccta cttgattgat gggactcaca agatcaccca gagcaatgcc atcctgcgcT 301 ACATTGCCCG CAAGCACAAC CTGTGTGGGG AGACAGAAGA GGAGAAGATT CGTGTGGACA 361 TTTTGGAGAA CCAGGTTATG GATAACCACA TGGAGCTGGT CAGACTGTGC TATGACCCAG 421 ATTTTGAGAA ACTGAAGCCA AAATACTTGG AGGAACTCCC TGAAAAGCTA AAGCTCTACT 481 CAGAGTTTCT GGGGAAGCGG CCATGGTTTG CAGGAGACAA GATCACCTTT GTGGATTTCC 541 TTGCCTATGA TGTCCTTGAC ATGAAGCGTA TATTTGAGCC CAAGTGCTTG GACGCCTTCC 601 TAAACTTGAA GGACTTCATC TCCCGCTTTG AGGGTTTGAA GAAGATCTCT GCCTACATGA 661 AGTCCAGCCA ATTCCTCCGA GGTCTTTTGT TTGGAAAGTC AGCTACATGG AACAGCAAAT 721 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGACAGTG ATATCGATAA TGCGTACCTA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt