Transcript: Human NM_001042496.2

Homo sapiens solute carrier family 12 member 6 (SLC12A6), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
SLC12A6 (9990)
Length:
7987
CDS:
465..3890

Additional Resources:

NCBI RefSeq record:
NM_001042496.2
NBCI Gene record:
SLC12A6 (9990)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001042496.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432191 GATGCATGACAGTGATATATC pLKO_005 3368 CDS 100% 13.200 18.480 N SLC12A6 n/a
2 TRCN0000042954 CCGAAACTCAATGCTACGATT pLKO.1 3497 CDS 100% 4.950 6.930 N SLC12A6 n/a
3 TRCN0000042953 CGGACATAAGAAAGCTCGAAA pLKO.1 758 CDS 100% 4.950 6.930 N SLC12A6 n/a
4 TRCN0000042956 GCTAAATAACATGCGTGTCTA pLKO.1 1379 CDS 100% 4.950 6.930 N SLC12A6 n/a
5 TRCN0000417234 TGAAACTCAACGAGGTTATAG pLKO_005 3700 CDS 100% 13.200 9.240 N SLC12A6 n/a
6 TRCN0000042957 CCCATGAAGCAAAGCTGGTTT pLKO.1 3730 CDS 100% 4.950 3.465 N SLC12A6 n/a
7 TRCN0000042955 CCGGTTTGCTTTGCTTCGATT pLKO.1 2651 CDS 100% 4.950 3.465 N SLC12A6 n/a
8 TRCN0000069258 GCCAAGGAAATACTTGTGTAA pLKO.1 5541 3UTR 100% 4.950 3.465 N Slc12a6 n/a
9 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 5263 3UTR 100% 4.950 2.475 Y CFLAR n/a
10 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 5263 3UTR 100% 4.950 2.475 Y C19orf31 n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 5901 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000069260 CCATTGAAATCTTTCTGGTAT pLKO.1 1297 CDS 100% 4.950 2.970 N Slc12a6 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 5902 3UTR 100% 13.200 6.600 Y LIAS n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6658 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 5261 3UTR 100% 4.950 2.475 Y ERN2 n/a
16 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 5261 3UTR 100% 4.950 2.475 Y P3H4 n/a
17 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 5261 3UTR 100% 4.950 2.475 Y P3H4 n/a
18 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6658 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001042496.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15687 pDONR223 0% 80.9% 80.6% None (many diffs) n/a
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