Construct: ORF ccsbBroadEn_15687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014146.1_s300c1, BRDN0000395230
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A6 (9990)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_001042494.2 | 84.5% | 84.3% | (many diffs) |
2 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_001042495.2 | 84.5% | 84.3% | (many diffs) |
3 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_005135.2 | 82% | 81.4% | (many diffs) |
4 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_001042496.2 | 80.9% | 80.6% | (many diffs) |
5 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_001365088.1 | 80.2% | 80% | (many diffs) |
6 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_133647.1 | 80.2% | 80% | (many diffs) |
7 | human | 9990 | SLC12A6 | solute carrier family 12 me... | NM_001042497.2 | 79% | 78.7% | (many diffs) |
8 | human | 9990 | SLC12A6 | solute carrier family 12 me... | XM_006720793.4 | 76% | 75.8% | (many diffs) |
9 | human | 9990 | SLC12A6 | solute carrier family 12 me... | XM_011522269.3 | 61.9% | 61.8% | 1_177del;1591_1592insTAG;2040_2041ins963 |
10 | human | 9990 | SLC12A6 | solute carrier family 12 me... | XR_931960.3 | 51.8% | (many diffs) | |
11 | mouse | 107723 | Slc12a6 | solute carrier family 12, m... | NM_133648.2 | 74.3% | 80% | (many diffs) |
12 | mouse | 107723 | Slc12a6 | solute carrier family 12, m... | XM_006498546.3 | 74.1% | 79.2% | (many diffs) |
13 | mouse | 107723 | Slc12a6 | solute carrier family 12, m... | NM_133649.2 | 72.6% | 78.4% | (many diffs) |
14 | mouse | 107723 | Slc12a6 | solute carrier family 12, m... | XM_006498545.3 | 71.4% | 77.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2898
- ORF length:
- 2829
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcaccat gagtgagatg tctggggcca ccacttcgct ggcaactgtt gcactggatc 121 cacccagtga ccggacttct cacccccagg atgtcatcga ggacctgagt cagaactcca 181 tcacagggga acacagccaa ctgttagacg acggacataa gaaagctcga aatgcttatc 241 tcaataattc caattatgaa gaaggagatg aatattttga taaaaatttg gcactctttg 301 aggaagaaat ggacaccaga ccgaaggtgt cttccctcct caaccgcatg gccaattaca 361 ctaatctgac tcaaggagca aaggaacatg aagaggcaga aaacatcact gaagggaaaa 421 agaagcccac caagaccccc caaatgggta ccttcatggg tgtctacctc ccatgtctac 481 aaaatatttt tggagtgatc ctttttttac gccttacatg ggtggtgggc acagctggag 541 ttcttcaggc ttttgcaatt gtccttatct gctgctgctg tacaatgttg actgctatct 601 ccatgagtgc cattgccact aatggagtgg tgccagctgg gggctcatac tttatgattt 661 cccgggcact gggcccagag tttggtgggg ctgttggcct ctgcttttat cttggtacca 721 catttgcagc agccatgtac atccttggtg ccattgaaat ctttctggtc tatatcgtcc 781 cccgagctgc catctttcac agtgatgacg cactcaagga atcagcagcc atgctaaata 841 acatgcgtgt ctacggcaca gctttcttgg tccttatggt attagtggta tttatcggcg 901 tacgctatgt gaacaagttt gcctcacttt tcctggcctg tgtcattgtg tccatcttgg 961 ccatctatgc tggagccatc aagtcttctt ttgctcctcc acacttcccg gtctgcatgc 1021 tgggtaaccg caccctttca tcaagacaca ttgacgtttg ctctaagacc aaggaaatta 1081 acaacatgac agtcccatca aagttatggg gattcttctg taactcgagt caatttttca 1141 atgccacctg tgatgaatac tttgttcaca ataacgtcac ttcaatccag ggcattcctg 1201 gattggctag tggtataatt acagagaatc tttggagtaa ttacctaccc aagggagaga 1261 tcatcgaaaa gccttcagcc aaatcttctg atgtcttagg cagcttaaac catgaatatg 1321 ttcttgttga catcaccacc tccttcacgc ttctggtggg aatcttcttt ccctctgtta 1381 caggtatcat ggctggatca aacagatctg gagatctgaa agatgctcag aagtctattc 1441 cgattggtac tatccttgcc atcctgacca cctcctttgt tttagattta agcaatgttg 1501 tcctttttgg tgcatgtatt gaaggggttg ttctcagaga caagttcggt gatgctgtga 1561 aaggtaattt ggtggtaggc accttatctt ggccatcccc atgggtgatt gttattggct 1621 ccttcttttc aacatgtggg gctggacttc agagcctcac aggtgcaccg aggctgctac 1681 aagctattgc caaggataac atcataccgt ttctgagggt ttttggccac agcaaagcca 1741 atggggaacc tacctgggct ttacttctaa ctgctgccat tgcagagctt ggaatactca 1801 ttgcctccct ggatcttgtg gccccaattc tttccatgtt ttttctcatg tgttacctct 1861 ttgtaaactt ggcatgtgcc ttgcaaacat tacttcgaac acccaactgg agaccccgat 1921 tccgctacta ccattgggcc ctttctttca tgggaatgag tatctgtctg gctctgatgt 1981 tcatttcttc ctggtattat gccattgtag ccatggtaat agctggtatg atctacaagt 2041 acattgaata ccaaggagct gagaaagaat ggggtgatgg tatccgtggg ctgtccctca 2101 gtgcagcccg gtttgctttg cttcgattgg aggaaggacc tccacacact aaaaactgga 2161 ggcctcagtt gcttgtatta ctgaaactag atgaagactt acatgtcaag catcctcgcc 2221 tcctcacctt tgcctcacag ctcaaagcag gaaaaggtct cactattgtg ggctctgtca 2281 tcgtggggaa cttcctagag aactacggtg aagctttagc tgctgagcag accataaagc 2341 acctaatgga ggcagagaag gtaaaaggat tctgccagct ggtggtggcc gccaagctga 2401 gggagggcat ttcccacctc atccagtcat gtggccttgg gggcatgaag cacaacacgg 2461 tggtgatggg ctggcctaat ggctggcgtc aaagcgaaga tgcccgcgct tggaagactt 2521 ttattggcac agttcgagtg acaactgctg cccatcttgc actgctggtg gctaaaaaca 2581 tctccttctt tcccagcaat gtggagcaat tttctgaggg caacattgat gtgtggtgga 2641 ttgtgcatga tggggggatg cttatgctac taccattcct actgaaacag cacaaggtgt 2701 ggcgaaagtg cagcatacgg atcttcacag tagcccaatt agaagacaac agtatccaaa 2761 tgaagaagga cctagccacc ttcctatatc acttacgcat tgaggcggag gtagaagtgg 2821 tggagatggt gagaaagctg agtttgagat acaagagcat gacagtgata tatcagcata 2881 tacttacgag cgcactttnn ccaactttct tgtacaaagt tggcattata agaaagcatt 2941 gcttatcaat ttgttgcaac gaac