Construct: ORF TRCN0000488327

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020548.1_s317c1
DNA Barcode:
AACTGCACTTAACAACCAAGGTCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAST2 (23139)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488327
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23139 MAST2 microtubule associated seri... XM_011541069.2 56.6% 52% (many diffs)
2 human 23139 MAST2 microtubule associated seri... XM_011541068.1 48.3% 44.5% (many diffs)
3 human 23139 MAST2 microtubule associated seri... XM_011541067.2 48.1% 44.3% (many diffs)
4 human 23139 MAST2 microtubule associated seri... NM_001324321.1 48.1% 44.3% (many diffs)
5 human 23139 MAST2 microtubule associated seri... XM_006710477.3 47.7% 44% (many diffs)
6 human 23139 MAST2 microtubule associated seri... XM_017000755.1 47.5% 43.8% (many diffs)
7 human 23139 MAST2 microtubule associated seri... XM_017000754.1 46.9% 43.2% (many diffs)
8 human 23139 MAST2 microtubule associated seri... XM_017000753.1 46.8% 43.1% (many diffs)
9 human 23139 MAST2 microtubule associated seri... XM_005270656.5 46.2% 42.5% (many diffs)
10 human 23139 MAST2 microtubule associated seri... XM_005270655.3 45.9% 42.3% (many diffs)
11 human 23139 MAST2 microtubule associated seri... XM_017000752.1 45.8% 42.2% (many diffs)
12 human 23139 MAST2 microtubule associated seri... XM_011541065.1 45.6% 42.1% (many diffs)
13 human 23139 MAST2 microtubule associated seri... NM_001319245.1 44% 40.5% (many diffs)
14 human 23139 MAST2 microtubule associated seri... NM_015112.3 43.9% 40.5% (many diffs)
15 human 23139 MAST2 microtubule associated seri... NM_001324320.1 43.8% 40.3% (many diffs)
16 human 23139 MAST2 microtubule associated seri... XM_011541064.2 43.5% 40.1% (many diffs)
17 human 23139 MAST2 microtubule associated seri... XM_011541063.1 43.4% 40% (many diffs)
18 human 23139 MAST2 microtubule associated seri... XM_011541062.2 42.4% 39.1% (many diffs)
19 human 23139 MAST2 microtubule associated seri... XM_011541061.2 42.3% 39% (many diffs)
20 human 23139 MAST2 microtubule associated seri... XM_011541059.2 42.2% 39% (many diffs)
21 mouse 17776 Mast2 microtubule associated seri... XM_006502836.3 42.3% 40.9% (many diffs)
22 mouse 17776 Mast2 microtubule associated seri... XM_006502835.3 42.1% 40.7% (many diffs)
23 mouse 17776 Mast2 microtubule associated seri... XM_006502834.2 40.7% 39.4% (many diffs)
24 mouse 17776 Mast2 microtubule associated seri... XM_017320018.1 40.1% 38.9% (many diffs)
25 mouse 17776 Mast2 microtubule associated seri... XM_006502840.2 38.9% 37.7% (many diffs)
26 mouse 17776 Mast2 microtubule associated seri... NM_008641.3 38.9% 37.7% (many diffs)
27 mouse 17776 Mast2 microtubule associated seri... NM_001042743.2 38.8% 37.6% (many diffs)
28 mouse 17776 Mast2 microtubule associated seri... XM_006502833.1 38.8% 37.5% (many diffs)
29 mouse 17776 Mast2 microtubule associated seri... XM_006502839.1 22.1% 18.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2448
ORF length:
2376
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcggcct cgctcccgga gcctcagtcc cggacgatcc ccagtatcct
121ttgacagtga aataataatg atgaatcatg tttacaaaga aagattccca aaggctcatg
181agcgctcaga gagctcagaa gtggcttttg tgatgcagct ggtgaaaaag ctgatgatta
241tcattgcccg cccagcacgt ctcctggaat gcctggagtt tgaccctgaa gagttctacc
301accttttaga agcagctgag ggccacgcca aagagggaca agggattaaa tgtgacattc
361cccgctacat cgttagccag ctgggcctca cccgggatcc cctagaagaa atggcccagt
421tgagcagctg tgacagtcct gacactccag agacagatga ttctattgag ggccatgggg
481catctctgcc atctaaaaag acaccctctg aagaggactt cgagaccatt aagctcatca
541gcaatggcgc ctatggggct gtatttctgg tgcggcacaa gtccacccgg cagcgctttg
601ccatgaagaa gatcaacaag cagaacctga tcctacggaa ccagatccag caggccttcg
661tggagcgtga catactgact ttcgctgaga acccctttgt ggtcagcatg ttctgctcct
721ttgataccaa gcgccacttg tgcatggtga tggagtacgt tgaaggggga gactgtgcca
781ctctgctgaa gaatattggg gccctgcctg tggacatggt gcgtctatac tttgcggaaa
841ctgtgctggc cctggagtac ttacacaact atggcatcgt gcaccgtgac ctcaagcctg
901acaacctcct aattacatcc atggggcaca tcaagctcac ggactttgga ctgtccaaaa
961tgggcctcat gagtctgaca acgaacttgt atgagggtca tattgaaaag gatgcccggg
1021aattcctgga caagcaggta tgcgggaccc cagaatacat tgcgcctgag gtgatcctgc
1081gccagggcta tgggaagcca gtggactggt gggccatggg cattatcctg tatgagttcc
1141tggtgggctg cgtccctttt tttggagata ctccggagga gctctttggg caggtgatca
1201gtgatgagat tgtgtggcct gagggtgatg aggcactgcc cccagacgcc caggacctca
1261cctccaaact gctccaccag aaccctctgg agagacttgg cacaggcagt gcctatgagg
1321tgaagcagca cccattcttt actggtctgg actggacagg acttctccgc cagaaggctg
1381aatttattcc tcagttggag tcagaggatg atactagcta ttttgacacc cgctcagagc
1441gataccacca catggactcg gaggatgagg aagaagtgag tgaggatggc tgccttgaga
1501tccgccagtt ctcttcctgc tctccaaggt tcaacaaggt gtacagcagc atggagcggc
1561tctcactgct cgaggagcgc cggacaccac ccccgaccaa gcgcagcctg agtgaggaga
1621aggaggacca ttcagatggc ctggcagggc tcaaaggccg agaccggagc tgggtgattg
1681gctcccctga gatattacgg aagcggctgt cggtgtctga gtcgtcccac acagagagtg
1741actcaagccc tccaatgaca gtgcgacgcc gctgctcagg cctcctggat gcgcctcggt
1801tcccggaggg ccctgaggag gccagcagca ccctcaggag gcaaccacag gagggtatat
1861gggtcctgac acccccatct ggagaggggg tatctgggcc tgtcactgaa cactcagggg
1921agcagcggcc aaagctggat gaggaagctg ttggccggag cagtggttcc agtccagcta
1981tggagacccg aggccgtggg acctcacagc tggctgaggg agccacagcc aaggccatca
2041gtgacctggc tgtgcgtagg gcccgccacc ggctgctctc tggggactca acagagaagc
2101gcactgctcg ccctgtcaac aaagtgatca agtccgccTC AGCCACAGCC CTCTCACTCC
2161TCATTCCTTC GGAACACCAC ACCTGCTCCC CGTTGGCCAG CCCCATGTCC CCACATTCTC
2221AGTCGTCCAA CCCATCATCC CGGGACTCTT CTCCAAGAAG TATGGCTTCA CCCTGCGGGC
2281CATTCGCGTC TACATGGGTG ACTCCGATGT CTACACCGTG CACCATATGG TGTGGCACGT
2341GGAGGATGGA GGTCCGGCCA GTGAGGCAGG GCTTCGTCAA GGTGACCTCA TCACCCATGT
2401CAATGGGGAA CCTGTGCATG GCCTGGTGCA CACGGAGGTG GTGGAGCTAG AACCCAGCTT
2461TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2521CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2581CTTGTGGAAA GGACGAAACT GCACTTAACA ACCAAGGTCT ACGCGTTAAG TCgacaatca
2641acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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