Transcript: Human NM_001111033.2

Homo sapiens activin A receptor type 1C (ACVR1C), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-07-23
Taxon:
Homo sapiens (human)
Gene:
ACVR1C (130399)
Length:
8382
CDS:
221..1231

Additional Resources:

NCBI RefSeq record:
NM_001111033.2
NBCI Gene record:
ACVR1C (130399)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146259 CTGGCGCTCTCAATTGCTAG pXPR_003 TGG 428 42% 3 -0.6574 ACVR1C ACVR1C 76522
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001111033.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196633 GAAGACTATATCTCAACTTTG pLKO.1 1186 CDS 100% 10.800 15.120 N ACVR1C n/a
2 TRCN0000194779 CCCTATTTCCTTTGTACATAC pLKO.1 2267 3UTR 100% 10.800 8.640 N ACVR1C n/a
3 TRCN0000196359 GAACCATATCTGTTGACATTT pLKO.1 2119 3UTR 100% 13.200 9.240 N ACVR1C n/a
4 TRCN0000195295 CTTTAGGTGATGCAATCTATG pLKO.1 1652 3UTR 100% 10.800 7.560 N ACVR1C n/a
5 TRCN0000220086 GCAACACCTCAACTCATCTTT pLKO.1 1451 3UTR 100% 5.625 3.938 N ACVR1C n/a
6 TRCN0000196393 GACTGAAGTGTGTATGTCTTT pLKO.1 294 CDS 100% 4.950 3.465 N ACVR1C n/a
7 TRCN0000197217 GCATCAGTCATGCTAACCAAT pLKO.1 362 CDS 100% 4.950 3.465 N ACVR1C n/a
8 TRCN0000197270 GCTCAAGTCTTCTGTCATAGT pLKO.1 431 CDS 100% 4.950 3.465 N ACVR1C n/a
9 TRCN0000220088 CGGAGGAATTGTTGAGGAGTA pLKO.1 970 CDS 100% 4.050 2.835 N ACVR1C n/a
10 TRCN0000220089 GCTCCTGAAATGCTTGATGAT pLKO.1 857 CDS 100% 4.950 2.970 N ACVR1C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001111033.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09527 pDONR223 100% 67.9% 67.9% None (many diffs) n/a
2 ccsbBroad304_09527 pLX_304 0% 67.9% 67.9% V5 (many diffs) n/a
3 TRCN0000467652 ATGGGACCATTCACCGAGACTTCT pLX_317 27.2% 67.9% 67.9% V5 (many diffs) n/a
4 ccsbBroadEn_15242 pDONR223 0% 67.9% 67.9% None (many diffs) n/a
5 ccsbBroad304_15242 pLX_304 0% 67.9% 67.9% V5 (many diffs) n/a
6 TRCN0000471789 AGCTGTCCGCTATGTCACGACGAA pLX_317 24.5% 67.9% 67.9% V5 (many diffs) n/a
7 TRCN0000489072 TATATCTAAAAGCAAGACTTGTCC pLX_317 25.2% 67.9% 67.9% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000487772 AAAATCACCTCTCCTATTAATCCT pLX_317 18.8% 67.9% 67.8% V5 (many diffs) n/a
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