Construct: ORF TRCN0000489431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021267.1_s317c1
- DNA Barcode:
- ATATAACCCGGTACCCCGACTGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CLCN2 (1181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XM_006713490.2 | 75.5% | 75.3% | 1_270del;452C>T;1151_1255del |
| 2 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_001171088.3 | 45.2% | 45.1% | 1_1296del;1478C>T;2177_2281del |
| 3 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XM_006713489.1 | 44.8% | 44.7% | 1_1428del;1610C>T |
| 4 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_001171087.3 | 43.6% | 43.2% | (many diffs) |
| 5 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_004366.6 | 43% | 42.9% | 1_1428del;1610C>T;2309_2413del |
| 6 | human | 1181 | CLCN2 | chloride voltage-gated chan... | NM_001171089.3 | 42.2% | 41.5% | (many diffs) |
| 7 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XR_001740002.1 | 39% | 1_1608del;1790C>T;2689_2690ins80 | |
| 8 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XM_011512401.1 | 38% | 35.3% | (many diffs) |
| 9 | human | 1181 | CLCN2 | chloride voltage-gated chan... | XR_001740001.1 | 37.5% | (many diffs) | |
| 10 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XM_006521749.3 | 51.5% | 54.6% | (many diffs) |
| 11 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | NM_009900.2 | 36.4% | 38.6% | (many diffs) |
| 12 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XM_017316862.1 | 36.1% | 38.3% | (many diffs) |
| 13 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XR_001781786.1 | 19.8% | (many diffs) | |
| 14 | mouse | 12724 | Clcn2 | chloride channel, voltage-s... | XR_384520.3 | 12.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1233
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgcc tggttcccag atggaattca tacggacagc agcacctacc 121 ggattgtgcc tgggggctac gctgtggtcg gggcagctgc gctggcagga gcggtgacac 181 acacagtgtc cacggctgtg atcgtgttcg agctcacagg ccagattgcc cacatcctgc 241 ctgtcatgat cgtcgtcatc ctggccaacg ctgtcgccca gagtctgcag ccctccctct 301 atgacagcat catccgaatc aagaaactgc cctacctgcc tgagctcggc tggggccgcc 361 accagcagta ccgggtgcgt gtggaggaca tcatggtgcg ggatgttccc catgtggccc 421 tcagctgcac cttccgggac ctgcgtttgg cactgcacag gaccaagggc cgaatgctgg 481 ccctagtgga gtcccctgag tccatgattc tgctgggctc catcgagcgt tcacaggtgg 541 tggcattgtt gggggcccag ctgagcccag cccgccggcg gcagcacatg caggagcgca 601 gagccaccca gacctctcca ctatctgatc aggagggtcc ccctacccct gaggcttctg 661 tctgcttcca ggtgaacaca gaagactcag ccttcccagc agcccggggg gagacccaca 721 agcccctaaa gcctgcactc aagagggggc ccagtgtcac caggaacctc ggagagagtc 781 ccacagggag cgcagagtcg gcaggcatcg ccctccggag ccTCTTCTGT GGCAGTCCAC 841 CCCCTGAGGC TGCTTCGGAG AAGTTGGAAT CCTGTGAGAA GCGCAAGCTG AAGCGTGTCC 901 GAATCTCCCT GGCAAGTGAC GCGGACCTGG AAGGCGAGAT GAGCCCTGAA GAGACTCACA 961 CTATCTTCTC ACTGCTGGGA GTGGACCATG CTTATGTCAC CAGTATTGGC AGACTCATTG 1021 GAATCGTTAC TCTAAAGGAG CTCCGGAAGG CCATCGAGGG CTCTGTCACA GCACAGGGTG 1081 TGAAAGTCCG GCCGCCCCTC GCCAGCTTCC GAGACAGTGC CACCAGCAGC AGTGACACGG 1141 AGACCACTGA GGTGCATGCA CTCTGGGGGC CCCACTCCCG TCATGGCCTC CCCCGGGAGG 1201 GCAGCCCTTC CGACAGCGAC GACAAATGCC AATAGAACCC AGCTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AATATAACCC GGTACCCCGA CTGAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt