Transcript: Human NM_001199505.1

Homo sapiens phosphatase and actin regulator 3 (PHACTR3), transcript variant 4, mRNA.

Source:
NCBI, updated 2018-06-25
Taxon:
Homo sapiens (human)
Gene:
PHACTR3 (116154)
Length:
2275
CDS:
1..1671

Additional Resources:

NCBI RefSeq record:
NM_001199505.1
NBCI Gene record:
PHACTR3 (116154)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001199505.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052689 GCGCTGAACGACTCCATTATT pLKO.1 1141 CDS 100% 15.000 21.000 N PHACTR3 n/a
2 TRCN0000417397 TGGAACACTGCCACGGAAATG pLKO_005 1164 CDS 100% 10.800 15.120 N PHACTR3 n/a
3 TRCN0000425136 GACCCACTGTTGATGAATTAA pLKO_005 1448 CDS 100% 15.000 12.000 N PHACTR3 n/a
4 TRCN0000430649 CTGATACGATTCAGTGATTAC pLKO_005 1483 CDS 100% 10.800 8.640 N PHACTR3 n/a
5 TRCN0000431962 TGATACCAACACTGAACATTC pLKO_005 1708 3UTR 100% 10.800 7.560 N PHACTR3 n/a
6 TRCN0000431986 TGCAATCTTGACCACACTTAC pLKO_005 1809 3UTR 100% 10.800 7.560 N PHACTR3 n/a
7 TRCN0000052690 CCAAGCAAACAGGAACTAGAA pLKO.1 1222 CDS 100% 4.950 3.465 N PHACTR3 n/a
8 TRCN0000052692 CGCTAAGGAATCTGAGGAGAA pLKO.1 1056 CDS 100% 4.050 2.835 N PHACTR3 n/a
9 TRCN0000052691 GCAGCAGATAAGGCAGCAATT pLKO.1 1567 CDS 100% 10.800 6.480 N PHACTR3 n/a
10 TRCN0000052688 GCTGTGTTTGTGAGAAGAGTA pLKO.1 1772 3UTR 100% 4.950 2.970 N PHACTR3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001199505.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04697 pDONR223 100% 95.7% 93.1% None (many diffs) n/a
2 ccsbBroad304_04697 pLX_304 0% 95.7% 93.1% V5 (many diffs) n/a
3 TRCN0000470159 CACGTGACTTTCCAAGATCCGTTG pLX_317 20.6% 95.7% 93.1% V5 (many diffs) n/a
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