Construct: ORF TRCN0000470159

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005269.1_s317c1
Derived from:
ccsbBroadEn_04697
DNA Barcode:
CACGTGACTTTCCAAGATCCGTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHACTR3 (116154)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470159
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 116154 PHACTR3 phosphatase and actin regul... NM_080672.5 100% 100%
2 human 116154 PHACTR3 phosphatase and actin regul... NM_001199505.1 95.7% 93.1% (many diffs)
3 human 116154 PHACTR3 phosphatase and actin regul... NM_001199506.1 92.6% 92.6% 0_1ins123
4 human 116154 PHACTR3 phosphatase and actin regul... NM_001281507.1 92.6% 92.6% 0_1ins123
5 human 116154 PHACTR3 phosphatase and actin regul... NM_183244.1 92.6% 92.6% 0_1ins123
6 human 116154 PHACTR3 phosphatase and actin regul... XM_011528525.2 92.6% 92.6% 0_1ins123
7 human 116154 PHACTR3 phosphatase and actin regul... XM_017027626.2 92.6% 92.6% 0_1ins123
8 human 116154 PHACTR3 phosphatase and actin regul... XM_017027627.2 92.6% 92.6% 0_1ins123
9 human 116154 PHACTR3 phosphatase and actin regul... XM_017027631.1 92.6% 92.6% 0_1ins123
10 human 116154 PHACTR3 phosphatase and actin regul... XM_011528526.2 87.4% 87.4% 538_539ins210
11 human 116154 PHACTR3 phosphatase and actin regul... XM_017027628.1 83.3% 80.8% (many diffs)
12 human 116154 PHACTR3 phosphatase and actin regul... NM_183246.1 80.1% 80.1% 0_1ins123;415_416ins210
13 human 116154 PHACTR3 phosphatase and actin regul... XM_017027629.2 80.1% 80.1% 0_1ins123;415_416ins210
14 human 116154 PHACTR3 phosphatase and actin regul... XM_017027630.1 80.1% 80.1% 0_1ins123;415_416ins210
15 mouse 74189 Phactr3 phosphatase and actin regul... NM_028806.2 86.7% 88.7% (many diffs)
16 mouse 74189 Phactr3 phosphatase and actin regul... NM_001177789.1 83.6% 82.5% (many diffs)
17 mouse 74189 Phactr3 phosphatase and actin regul... NM_001177790.1 83.5% 82.3% (many diffs)
18 mouse 74189 Phactr3 phosphatase and actin regul... NM_001177791.1 82.1% 81.3% (many diffs)
19 mouse 74189 Phactr3 phosphatase and actin regul... NM_001007154.3 80.1% 82.2% (many diffs)
20 mouse 74189 Phactr3 phosphatase and actin regul... XM_006500730.2 80.1% 82.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1746
ORF length:
1677
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggccgcgtcg gaggacggga gcggctgcct cgtgtcgcgg ggccgctcgc
121agagtgaccc cagcgtcctc accgactcct cggccacctc ctccgcggac gccggggaga
181acccagatga gatggaccaa acgcccccgg cgcgtcctga atatctggtc tcagggattc
241gaactccccc tgtgaggagg aacagcaaac tggccaccct gggcaggatc ttcaaaccct
301ggaaatggag gaaaaagaaa aacgaaaaac tgaagcagac aacgtcagcg ctggagaaga
361agatggccgg caggcaaggc cgagaggagc tcatcaagaa ggggctgctg gagatgatgg
421agcaggatgc tgaaagcaaa acttgcaacc ccgatggagg accccgatct gtacagagtg
481aaccacccac tcccaagtcg gagacgctga cttcagaaga tgcccagccc ggaagcccct
541tggccactgg gacggaccag gtctccctgg acaagccact gtcctcagct gcccacttgg
601acgatgcagc caagatgcct tctgcatcca gtggtgaaga agcagacgct ggcagcctcc
661tgcccaccac caatgagctc tcccaagcct tagctggggc tgactccctg gacagtcctc
721ccagacctct ggagagatcc gtgggccagc tccccagccc cccactgctg cccactccgc
781cacccaaggc aagctccaaa accacaaaaa atgtcacagg ccaagccaca ctcttccaag
841cctccagcat gaagagtgcc gacccttccc tccggggcca gctctccaca cccacggggt
901ctccgcatct caccacggtc caccggcctc ttcccccaag ccgcgtcatt gaggagctgc
961acagggcgct ggccacgaag caccgccagg acagttttca aggaagagaa agtaaagggt
1021ctccaaagaa gcggctggat gtccgtctgt cgagaacgtc cagcgtggag cggggcaagg
1081agagggagga ggcttggagc tttgacgggg cattggagaa caagcgaact gccgctaagg
1141aatctgagga gaacaaggag aacctgatca taaattctga actcaaagac gacttgcttt
1201tgtatcagga cgaggaggcg ctgaacgact ccattatttc tggaacactg ccacggaaat
1261gcaagaagga gctcctggcc gtgaagctaa ggaaccggcc aagcaaacag gaactagaag
1321accggaacat tttccccaga aggactgatg aagaaagaca ggagatccgg cagcagatcg
1381agatgaagct ttccaaacgg ctgagCCAAA GACCTGCCGT GGAAGAGCTG GAGAGAAGAA
1441ATATCTTGAA ACAAAGGAAT GATCAGACAG AGCAGGAAGA AAGAAGAGAA ATCAAGCAAA
1501GATTGACAAG AAAGCTTAAT CAGAGACCCA CTGTTGATGA ATTAAGAGAC AGAAAAATTC
1561TGATACGATT CAGTGATTAC GTGGAAGTAG CAAAAGCGCA GGACTATGAC AGGAGGGCAG
1621ACAAACCCTG GACGAGACTG TCAGCAGCAG ATAAGGCAGC AATTCGTAAA GAATTAAATG
1681AGTACAAAAG TAATGAAATG GAGGTACATG CATCAAGCAA GCACTTGACA AGATTCCACA
1741GGCCATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1801TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1861CTTGGCTTTA TATATCTTGT GGAAAGGACG ACACGTGACT TTCCAAGATC CGTTGACGCG
1921TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)