Construct: ORF TRCN0000470159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005269.1_s317c1
- Derived from:
- ccsbBroadEn_04697
- DNA Barcode:
- CACGTGACTTTCCAAGATCCGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHACTR3 (116154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116154 | PHACTR3 | phosphatase and actin regul... | NM_080672.5 | 100% | 100% | |
| 2 | human | 116154 | PHACTR3 | phosphatase and actin regul... | NM_001199505.1 | 95.7% | 93.1% | (many diffs) |
| 3 | human | 116154 | PHACTR3 | phosphatase and actin regul... | NM_001199506.1 | 92.6% | 92.6% | 0_1ins123 |
| 4 | human | 116154 | PHACTR3 | phosphatase and actin regul... | NM_001281507.1 | 92.6% | 92.6% | 0_1ins123 |
| 5 | human | 116154 | PHACTR3 | phosphatase and actin regul... | NM_183244.1 | 92.6% | 92.6% | 0_1ins123 |
| 6 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_011528525.2 | 92.6% | 92.6% | 0_1ins123 |
| 7 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_017027626.2 | 92.6% | 92.6% | 0_1ins123 |
| 8 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_017027627.2 | 92.6% | 92.6% | 0_1ins123 |
| 9 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_017027631.1 | 92.6% | 92.6% | 0_1ins123 |
| 10 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_011528526.2 | 87.4% | 87.4% | 538_539ins210 |
| 11 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_017027628.1 | 83.3% | 80.8% | (many diffs) |
| 12 | human | 116154 | PHACTR3 | phosphatase and actin regul... | NM_183246.1 | 80.1% | 80.1% | 0_1ins123;415_416ins210 |
| 13 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_017027629.2 | 80.1% | 80.1% | 0_1ins123;415_416ins210 |
| 14 | human | 116154 | PHACTR3 | phosphatase and actin regul... | XM_017027630.1 | 80.1% | 80.1% | 0_1ins123;415_416ins210 |
| 15 | mouse | 74189 | Phactr3 | phosphatase and actin regul... | NM_028806.2 | 86.7% | 88.7% | (many diffs) |
| 16 | mouse | 74189 | Phactr3 | phosphatase and actin regul... | NM_001177789.1 | 83.6% | 82.5% | (many diffs) |
| 17 | mouse | 74189 | Phactr3 | phosphatase and actin regul... | NM_001177790.1 | 83.5% | 82.3% | (many diffs) |
| 18 | mouse | 74189 | Phactr3 | phosphatase and actin regul... | NM_001177791.1 | 82.1% | 81.3% | (many diffs) |
| 19 | mouse | 74189 | Phactr3 | phosphatase and actin regul... | NM_001007154.3 | 80.1% | 82.2% | (many diffs) |
| 20 | mouse | 74189 | Phactr3 | phosphatase and actin regul... | XM_006500730.2 | 80.1% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1746
- ORF length:
- 1677
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgcgtcg gaggacggga gcggctgcct cgtgtcgcgg ggccgctcgc 121 agagtgaccc cagcgtcctc accgactcct cggccacctc ctccgcggac gccggggaga 181 acccagatga gatggaccaa acgcccccgg cgcgtcctga atatctggtc tcagggattc 241 gaactccccc tgtgaggagg aacagcaaac tggccaccct gggcaggatc ttcaaaccct 301 ggaaatggag gaaaaagaaa aacgaaaaac tgaagcagac aacgtcagcg ctggagaaga 361 agatggccgg caggcaaggc cgagaggagc tcatcaagaa ggggctgctg gagatgatgg 421 agcaggatgc tgaaagcaaa acttgcaacc ccgatggagg accccgatct gtacagagtg 481 aaccacccac tcccaagtcg gagacgctga cttcagaaga tgcccagccc ggaagcccct 541 tggccactgg gacggaccag gtctccctgg acaagccact gtcctcagct gcccacttgg 601 acgatgcagc caagatgcct tctgcatcca gtggtgaaga agcagacgct ggcagcctcc 661 tgcccaccac caatgagctc tcccaagcct tagctggggc tgactccctg gacagtcctc 721 ccagacctct ggagagatcc gtgggccagc tccccagccc cccactgctg cccactccgc 781 cacccaaggc aagctccaaa accacaaaaa atgtcacagg ccaagccaca ctcttccaag 841 cctccagcat gaagagtgcc gacccttccc tccggggcca gctctccaca cccacggggt 901 ctccgcatct caccacggtc caccggcctc ttcccccaag ccgcgtcatt gaggagctgc 961 acagggcgct ggccacgaag caccgccagg acagttttca aggaagagaa agtaaagggt 1021 ctccaaagaa gcggctggat gtccgtctgt cgagaacgtc cagcgtggag cggggcaagg 1081 agagggagga ggcttggagc tttgacgggg cattggagaa caagcgaact gccgctaagg 1141 aatctgagga gaacaaggag aacctgatca taaattctga actcaaagac gacttgcttt 1201 tgtatcagga cgaggaggcg ctgaacgact ccattatttc tggaacactg ccacggaaat 1261 gcaagaagga gctcctggcc gtgaagctaa ggaaccggcc aagcaaacag gaactagaag 1321 accggaacat tttccccaga aggactgatg aagaaagaca ggagatccgg cagcagatcg 1381 agatgaagct ttccaaacgg ctgagCCAAA GACCTGCCGT GGAAGAGCTG GAGAGAAGAA 1441 ATATCTTGAA ACAAAGGAAT GATCAGACAG AGCAGGAAGA AAGAAGAGAA ATCAAGCAAA 1501 GATTGACAAG AAAGCTTAAT CAGAGACCCA CTGTTGATGA ATTAAGAGAC AGAAAAATTC 1561 TGATACGATT CAGTGATTAC GTGGAAGTAG CAAAAGCGCA GGACTATGAC AGGAGGGCAG 1621 ACAAACCCTG GACGAGACTG TCAGCAGCAG ATAAGGCAGC AATTCGTAAA GAATTAAATG 1681 AGTACAAAAG TAATGAAATG GAGGTACATG CATCAAGCAA GCACTTGACA AGATTCCACA 1741 GGCCATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1801 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1861 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACACGTGACT TTCCAAGATC CGTTGACGCG 1921 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt