Transcript: Mouse NM_001205386.1

Mus musculus ARP3 actin-related protein 3 (Actr3), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Actr3 (74117)
Length:
2551
CDS:
205..1461

Additional Resources:

NCBI RefSeq record:
NM_001205386.1
NBCI Gene record:
Actr3 (74117)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001205386.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380720 GACGCTGACGGGTACAGTAAT pLKO_005 687 CDS 100% 13.200 18.480 N Actr3 n/a
2 TRCN0000294912 TGGACTGTGGCACGGGATATA pLKO_005 233 CDS 100% 13.200 18.480 N Actr3 n/a
3 TRCN0000379668 GGCCAATTCGCCATGGTATAG pLKO_005 431 CDS 100% 10.800 15.120 N Actr3 n/a
4 TRCN0000090240 GCCATCTCAAAGAAAGAGTTT pLKO.1 985 CDS 100% 4.950 3.960 N Actr3 n/a
5 TRCN0000294914 CAATCGCAGGAAGAGATATAA pLKO_005 785 CDS 100% 15.000 10.500 N Actr3 n/a
6 TRCN0000090242 CCCATCATGTATTGCCATTAA pLKO.1 297 CDS 100% 13.200 9.240 N Actr3 n/a
7 TRCN0000294913 GCCTATTGATGTACAAGTTAT pLKO_005 1287 CDS 100% 13.200 9.240 N Actr3 n/a
8 TRCN0000090241 TCCCATCATGTATTGCCATTA pLKO.1 296 CDS 100% 10.800 7.560 N Actr3 n/a
9 TRCN0000090239 GAACCTGAAGATCATTACTTT pLKO.1 514 CDS 100% 5.625 3.938 N Actr3 n/a
10 TRCN0000287540 GAACCTGAAGATCATTACTTT pLKO_005 514 CDS 100% 5.625 3.938 N Actr3 n/a
11 TRCN0000090238 GCAGATGTAGAAGAGAGCTAA pLKO.1 1600 3UTR 100% 4.950 2.970 N Actr3 n/a
12 TRCN0000287539 GCAGATGTAGAAGAGAGCTAA pLKO_005 1600 3UTR 100% 4.950 2.970 N Actr3 n/a
13 TRCN0000029381 GCCATGGTATAGTTGAAGATT pLKO.1 440 CDS 100% 5.625 3.938 N ACTR3 n/a
14 TRCN0000343278 GCCATGGTATAGTTGAAGATT pLKO_005 440 CDS 100% 5.625 3.938 N ACTR3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001205386.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02313 pDONR223 100% 93.4% 99.7% None (many diffs) n/a
2 ccsbBroad304_02313 pLX_304 0% 93.4% 99.7% V5 (many diffs) n/a
3 TRCN0000467535 CCCCTGACTGGTTGATGGTACCGA pLX_317 28.8% 93.4% 99.7% V5 (many diffs) n/a
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