Construct: ORF TRCN0000467535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014282.1_s317c1
- Derived from:
- ccsbBroadEn_02313
- DNA Barcode:
- CCCCTGACTGGTTGATGGTACCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTR3 (10096)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10096 | ACTR3 | actin related protein 3 | NM_005721.5 | 100% | 100% | |
| 2 | human | 10096 | ACTR3 | actin related protein 3 | NM_001277140.1 | 87.7% | 87.7% | 0_1ins153 |
| 3 | human | 57180 | ACTR3B | actin related protein 3B | NM_020445.6 | 76.8% | 90.6% | (many diffs) |
| 4 | human | 10096 | ACTR3 | actin related protein 3 | NR_102318.1 | 19.7% | 1_346del;446_447ins125;1476_5583del | |
| 5 | mouse | 74117 | Actr3 | ARP3 actin-related protein 3 | NM_001205385.1 | 93.4% | 99.7% | (many diffs) |
| 6 | mouse | 74117 | Actr3 | ARP3 actin-related protein 3 | NM_001205386.1 | 93.4% | 99.7% | (many diffs) |
| 7 | mouse | 74117 | Actr3 | ARP3 actin-related protein 3 | NM_023735.2 | 93.4% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggacggctg ccggcctgtg tggtggactg tggcacgggg tatacaaaac 121 taggatatgc tggaaataca gaaccacagt ttatcatccc ttcctgtatt gctattaagg 181 agtcagcaaa agtgggtgat caagctcaaa ggagggtgat gaaaggtgtt gatgacctag 241 acttcttcat tggtgatgaa gcaatagaaa aacctacata tgcaacaaag tggccaatcc 301 gccatggtat agttgaagat tgggacttaa tggaaaggtt tatggagcaa gtgatcttta 361 aatatttaag ggcagaacct gaagaccatt attttctttt gactgaacct ccattgaata 421 ctccagaaaa cagggaatat actgctgaaa taatgtttga gtccttcaat gttccaggct 481 tgtacattgc tgtgcaggct gttcttgcct tagctgcatc ttggacctca agacaagtag 541 gagaacggac gttgaccggt acggtaatag acagtggaga tggtgtcact catgtcattc 601 ctgtggctga agggtatgtg attggcagct gtattaaaca cattccaatc gcaggacgag 661 atataacata ttttattcag caactgctga gagaccgaga agtaggaatc cctccagaac 721 aatccttgga aactgctaag gcagtaaagg agcgctatag ttatgtctgc ccagatttag 781 taaaagaatt taacaagtat gatacagatg ggtcaaaatg gattaaacag tatactggaa 841 tcaatgctat ctcaaagaaa gagttttcta tcgatgttgg ttatgagaga tttttgggac 901 ctgaaatctt ttttcatcca gagtttgcta atccagactt tacacaacct atctcagaag 961 ttGTAGATGA AGTAATTCAG AATTGTCCTA TTGATGTCAG ACGTCCTCTC TACAAGAATA 1021 TTGTCCTCTC TGGAGGTTCA ACCATGTTCA GGGACTTTGG ACGTCGCTTG CAAAGAGATT 1081 TGAAAAGAAC TGTAGATGCC CGGCTGAAAT TAAGTGAGGA ATTGAGTGGT GGTAGATTGA 1141 AGCCAAAACC TATTGATGTA CAAGTCATTA CACACCACAT GCAGCGATAT GCAGTTTGGT 1201 TTGGAGGATC AATGCTGGCT TCCACGCCTG AGTTCTACCA AGTATGCCAC ACCAAAAAGG 1261 ATTATGAAGA AATTGGACCT AGCATTTGTC GTCACAATCC AGTGTTTGGA GTCATGTCGT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGACCCCT GACTGGTTGA TGGTACCGAA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt