Construct: ORF TRCN0000489356

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021403.1_s317c1
DNA Barcode:
CACTGAGATTGGACCATACTAGGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAP4K4 (9448)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489356
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9448 MAP4K4 mitogen-activated protein k... XM_017005364.1 99.7% 99.8% 161A>G;1808_1809insCAG;3747_3748insTAGAC
2 human 9448 MAP4K4 mitogen-activated protein k... XM_017005363.1 99.6% 99.6% 161A>G;2062_2070delGGCGAAGTG;3759_3760insTAGAC
3 human 9448 MAP4K4 mitogen-activated protein k... XM_005264064.3 99.2% 99.2% 161A>G;3370_3393del;3774_3775insTAGAC
4 human 9448 MAP4K4 mitogen-activated protein k... XM_005264062.3 98.9% 99% (many diffs)
5 human 9448 MAP4K4 mitogen-activated protein k... XM_005264063.3 98.8% 98.9% (many diffs)
6 human 9448 MAP4K4 mitogen-activated protein k... XM_006712868.2 97.1% 97.2% (many diffs)
7 human 9448 MAP4K4 mitogen-activated protein k... XM_005264068.3 96.7% 96.8% (many diffs)
8 human 9448 MAP4K4 mitogen-activated protein k... XM_005264065.3 96.5% 96.5% (many diffs)
9 human 9448 MAP4K4 mitogen-activated protein k... XM_005264066.3 96.4% 96.5% (many diffs)
10 human 9448 MAP4K4 mitogen-activated protein k... XM_017005352.1 95.4% 95.5% (many diffs)
11 human 9448 MAP4K4 mitogen-activated protein k... XM_017005353.1 95.4% 95.4% (many diffs)
12 human 9448 MAP4K4 mitogen-activated protein k... XM_005264058.3 95.1% 95.1% (many diffs)
13 human 9448 MAP4K4 mitogen-activated protein k... XM_017005351.1 95% 95.1% (many diffs)
14 human 9448 MAP4K4 mitogen-activated protein k... XM_005264057.3 94.9% 94.9% (many diffs)
15 human 9448 MAP4K4 mitogen-activated protein k... XM_017005371.1 94.7% 94.8% 161A>G;2356_2357ins192;3558_3559insTAGAC
16 human 9448 MAP4K4 mitogen-activated protein k... XM_005264071.2 94.6% 94.7% (many diffs)
17 human 9448 MAP4K4 mitogen-activated protein k... XM_006712869.2 94.5% 94.5% (many diffs)
18 human 9448 MAP4K4 mitogen-activated protein k... XM_017005370.1 94.4% 94.4% (many diffs)
19 human 9448 MAP4K4 mitogen-activated protein k... XM_017005350.1 93.8% 93.9% (many diffs)
20 human 9448 MAP4K4 mitogen-activated protein k... XM_017005369.1 93.8% 93.8% (many diffs)
21 human 9448 MAP4K4 mitogen-activated protein k... NM_145686.3 93.3% 93.4% (many diffs)
22 human 9448 MAP4K4 mitogen-activated protein k... XM_005264050.3 93.2% 93.3% (many diffs)
23 human 9448 MAP4K4 mitogen-activated protein k... XM_005264052.3 93.2% 93.2% (many diffs)
24 human 9448 MAP4K4 mitogen-activated protein k... XM_017005357.1 93.1% 93.1% (many diffs)
25 human 9448 MAP4K4 mitogen-activated protein k... XM_017005358.1 93% 93.1% (many diffs)
26 human 9448 MAP4K4 mitogen-activated protein k... XM_017005356.1 92.7% 92.8% (many diffs)
27 human 9448 MAP4K4 mitogen-activated protein k... XM_005264060.3 92.6% 92.7% (many diffs)
28 human 9448 MAP4K4 mitogen-activated protein k... XM_005264059.3 92.5% 92.6% (many diffs)
29 human 9448 MAP4K4 mitogen-activated protein k... XM_017005375.1 92.2% 92.3% (many diffs)
30 human 9448 MAP4K4 mitogen-activated protein k... XM_017005374.1 92% 92% (many diffs)
31 human 9448 MAP4K4 mitogen-activated protein k... XM_017005355.1 91.7% 91.7% (many diffs)
32 human 9448 MAP4K4 mitogen-activated protein k... XM_017005373.1 91.6% 91.7% (many diffs)
33 human 9448 MAP4K4 mitogen-activated protein k... XM_017005354.1 91.5% 91.5% (many diffs)
34 human 9448 MAP4K4 mitogen-activated protein k... XM_017005372.1 91.4% 91.5% (many diffs)
35 human 9448 MAP4K4 mitogen-activated protein k... NM_004834.5 91.3% 91.4% (many diffs)
36 human 9448 MAP4K4 mitogen-activated protein k... XM_006712865.2 90.9% 91% (many diffs)
37 human 9448 MAP4K4 mitogen-activated protein k... NM_001242559.2 90.7% 90.7% (many diffs)
38 human 9448 MAP4K4 mitogen-activated protein k... XM_017005365.1 90.6% 90.6% (many diffs)
39 human 9448 MAP4K4 mitogen-activated protein k... XM_005264048.2 90.1% 90.2% (many diffs)
40 human 9448 MAP4K4 mitogen-activated protein k... XM_017005361.1 89% 89% (many diffs)
41 human 9448 MAP4K4 mitogen-activated protein k... XM_017005362.1 88.9% 89% (many diffs)
42 human 9448 MAP4K4 mitogen-activated protein k... XM_017005359.1 88.5% 88.5% (many diffs)
43 human 9448 MAP4K4 mitogen-activated protein k... XM_005264069.2 88.4% 88.4% (many diffs)
44 human 9448 MAP4K4 mitogen-activated protein k... XM_017005360.1 88.4% 88.4% (many diffs)
45 human 9448 MAP4K4 mitogen-activated protein k... NM_145687.4 88.2% 88.2% (many diffs)
46 human 9448 MAP4K4 mitogen-activated protein k... XM_017005368.1 88.1% 88.2% (many diffs)
47 human 9448 MAP4K4 mitogen-activated protein k... XM_017005349.1 87.8% 87.9% (many diffs)
48 human 9448 MAP4K4 mitogen-activated protein k... NM_001242560.2 86.7% 86.7% (many diffs)
49 human 9448 MAP4K4 mitogen-activated protein k... XM_017005367.1 86.6% 86.6% (many diffs)
50 human 9448 MAP4K4 mitogen-activated protein k... XM_017005366.1 86.1% 86.2% (many diffs)
51 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321021.1 90.5% 98.2% (many diffs)
52 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321018.1 90.3% 98% (many diffs)
53 mouse 26921 Map4k4 mitogen-activated protein k... NM_001252201.1 87.8% 95.5% (many diffs)
54 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321011.1 86.6% 93.9% (many diffs)
55 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321013.1 85.3% 90% (many diffs)
56 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321004.1 85.2% 92.4% (many diffs)
57 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496042.1 85.1% 92.3% (many diffs)
58 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496043.1 84.4% 91.8% (many diffs)
59 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321016.1 84.2% 91.5% (many diffs)
60 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496046.1 82.7% 90.3% (many diffs)
61 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321002.1 82.1% 86.4% (many diffs)
62 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321001.1 81.9% 88.9% (many diffs)
63 mouse 26921 Map4k4 mitogen-activated protein k... XM_017320998.1 81.9% 88.8% (many diffs)
64 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496044.1 81.9% 89.1% (many diffs)
65 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496041.1 81.8% 88.7% (many diffs)
66 mouse 26921 Map4k4 mitogen-activated protein k... XM_017320994.1 81.7% 88.6% (many diffs)
67 mouse 26921 Map4k4 mitogen-activated protein k... NM_008696.2 80.4% 85.1% (many diffs)
68 mouse 26921 Map4k4 mitogen-activated protein k... NM_001252202.1 79.6% 86.8% (many diffs)
69 mouse 26921 Map4k4 mitogen-activated protein k... XM_006496045.1 79.4% 86.6% (many diffs)
70 mouse 26921 Map4k4 mitogen-activated protein k... NM_001252200.1 77.5% 81.8% (many diffs)
71 mouse 26921 Map4k4 mitogen-activated protein k... XM_017321010.1 77.4% 84.2% (many diffs)
72 mouse 26921 Map4k4 mitogen-activated protein k... XM_011238530.1 77.2% 84.1% (many diffs)
73 mouse 26921 Map4k4 mitogen-activated protein k... XR_001784730.1 57.1% (many diffs)
74 mouse 26921 Map4k4 mitogen-activated protein k... XR_001784729.1 55% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
3843
ORF length:
3750
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcacctct agaaaaggga aaatggcgaa cgactcccct gcaaaaagtc
121tggtggacat cgacctctcc tccctgcggg atcctgctgg gatttttgag ctggtggaag
181tggttggaaa tggcacctat ggacaagtct ataagggtcg acatgttaaa acgggtcagt
241tggcagccat cagagttatg gatgtcactg aggatgaaga ggaagaaatc aaactggaga
301taaatatgct aaagaaatac tctcatcaca gaaacattgc aacatattat ggtgctttca
361tcaaaaagag ccctccagga catgatgacc aactctggct tgttatggag ttctgtgggg
421ctgggtccat tacagacctt gtgaagaaca ccaaagggaa cacactcaaa gaagactgga
481tcgcttacat ctccagagaa atcctgaggg gactggcaca tcttcacatt catcatgtga
541ttcaccggga tatcaagggc cagaatgtgt tgctgactga gaatgcagag gtgaaacttg
601ttgactttgg tgtgagtgct cagctggaca ggactgtggg gcggagaaat acgttcatag
661gcactcccta ctggatggct cctgaggtca tcgcctgtga tgagaaccca gatgccacct
721atgattacag aagtgatctt tggtcttgtg gcattacagc cattgagatg gcagaaggtg
781ctccccctct ctgtgacatg catccaatga gagcactgtt tctcattccc agaaaccctc
841ctccccggct gaagtcaaaa aaatggtcga agaagttttt tagttttata gaagggtgcc
901tggtgaagaa ttacatgcag cggccctcta cagagcagct tttgaaacat ccttttataa
961gggatcagcc aaatgaaagg caagttagaa tccagcttaa ggatcatata gatcgtacca
1021ggaagaagag aggcgagaaa gatgaaactg agtatgagta cagtgggagt gaggaagaag
1081aggaggaagt gcctgaacag gaaggagagc caagttccat tgtgaacgtg cctggtgagt
1141ctactcttcg ccgagatttc ctgagactgc agcaggagaa caaggaacgt tccgaggctc
1201ttcggagaca acagttacta caggagcaac agctccggga gcaggaagaa tataaaaggc
1261aactgctggc agagagacag aagcggattg agcagcagaa agaacagagg cgacggctag
1321aagagcaaca aaggagagag cgggaagcta gaaggcagca ggaacgtgaa cagcgaagga
1381gagaacaaga agaaaagagg cgtctagagg agttggagag aaggcgcaaa gaagaagagg
1441agaggagacg ggcagaagaa gaaaagagga gagttgaaag agaacaggag tatatcaggc
1501gacagctaga agaggagcag cggcacttgg aagtccttca gcagcagctg ctccaggagc
1561aggccatgtt actggagtgc cgatggcggg agatggagga gcaccggcag gcagagaggc
1621tccagaggca gttgcaacaa gaacaagcat atctcctgtc tctacagcat gaccatagga
1681ggccgcaccc gcagcactcg cagcagccgc caccaccgca gcaggaaagg agcaagccaa
1741gcttccatgc tcccgagccc aaagcccact acgagcctgc tgaccgagcg cgagaggttc
1801ctgtgagaac aacatctcgc tcccctgttc tgtcccgtcg agattcccca ctgcagggca
1861gtgggcagca gaatagccag gcaggacaga gaaactccac cagcagtatt gagcccaggc
1921ttctgtggga gagagtggag aagctggtgc ccagacctgg cagtggcagc tcctcagggt
1981ccagcaactc aggatcccag cccgggtctc accctgggtc tcagagtggc tccggggaac
2041gcttcagagt gagatcatca tccaagtctg aaggctctcc atctcagcgc ctggaaaatg
2101cagtgaaaaa acctgaagat aaaaaggaag ttttcagacc cctcaagcct gctgatctga
2161ccgcactggc caaagagctt cgagcagtgg aagatgtacg gccacctcac aaagtaacgg
2221actactcctc atccagtgag gagtcgggga cgacggatga ggaggacgac gatgtggagc
2281aggaaggggc tgacgagtcc acctcaggac cagaggacac cagagcagcg tcatctctga
2341atttgagcaa tggtgaaacg gaatctgtga aaaccatgat tgtccatgat gatgtagaaa
2401gtgagccggc catgacccca tccaaggagg gcactctaat cgtccgccag agtacagttg
2461accaaaagcg tgccagccat catgagagca atggctttgc cggtcgcatt cacctcttgc
2521cagatctctt acagcaaagc cattcctcct ccacttcctc cacctcctcc tccccatcct
2581ccagccagcc gacacccacc atgtccccac agacacccca ggacaagctc actgctaatg
2641agactcagtc cgctagtagc acactccaga aacacaaatc ttcctcctcc tttacacctt
2701ttatagaccc cagattacta cagatttctc catctagcgg aacaacagtg acatctgtgg
2761tgggattttc ctgtgatggg atgagaccag aagccataag gcaagatcct acccggaaag
2821gctcagtggt caatgtgaat cctaccaaca ctaggccaca gagtgacacc ccggagattc
2881gtaaatacaa gaagaggttt aactctgaga ttctgtgtgc tgccttatgg ggagtgaatt
2941tgctagtggg tacagagagt ggcctgatgc tgctggacag aagtggccaa gggaaggtct
3001atcctcttat caaccgaaga cgatttcaac aaatggacgt acttgagggc ttgaatgtct
3061tggtgacaat atctggcaaa aaggataagt tacgtgtcta ctatttgtcc tggttaagaa
3121ataaaatact tcacaatgat ccagaagttg agaagaagca gggatggaca accgtagggg
3181atttggaagg atgtgtacat tataaagttg taaaatatga aagaatcaaa tttctggtga
3241ttgctttgaa gagttctgtg gaagtctatg cgtgggcacc aaagccatat cacaaattta
3301tggcctttaa gtcatttgga gaattggtac ataagccatt actggtggat ctcactgttg
3361aggaaggcca gaggttgaaa gtgatctatg gatcctgtgc tggattccat gctgttgatg
3421tggattcagg atcagtctat gacatttatc taccaacaca tatccagtgt agcatcaaac
3481cccatgcaat catcatcctc cccaatacag atggaatgga gcttctggtg tgctatgaag
3541atgagggggt ttatgtaaac acatatGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG
3601GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA
3661AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA
3721GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC
3781GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT
3841GGTAGACTAG TAAGGGTGGG CGCGCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
3901GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3961AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACACTGAGA
4021TTGGACCATA CTAGGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
4081aagatt

Download FASTA (ORF) (Full)