Construct: ORF TRCN0000469902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017273.1_s317c1
- Derived from:
- ccsbBroadEn_00654
- DNA Barcode:
- TCTGGTCGCGCACGCAAGCGGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNB1 (2782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2782 | GNB1 | G protein subunit beta 1 | NM_001282539.1 | 100% | 100% | |
| 2 | human | 2782 | GNB1 | G protein subunit beta 1 | NM_002074.5 | 100% | 100% | |
| 3 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_017001059.2 | 100% | 100% | |
| 4 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_017001060.2 | 100% | 100% | |
| 5 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_017001061.2 | 100% | 100% | |
| 6 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_024446495.1 | 100% | 100% | |
| 7 | human | 2783 | GNB2 | G protein subunit beta 2 | NM_005273.4 | 78.6% | 90.2% | (many diffs) |
| 8 | human | 59345 | GNB4 | G protein subunit beta 4 | NM_021629.4 | 75.9% | 90.8% | (many diffs) |
| 9 | human | 2782 | GNB1 | G protein subunit beta 1 | NM_001282538.1 | 70.5% | 70.5% | 0_1ins300 |
| 10 | mouse | 14688 | Gnb1 | guanine nucleotide binding ... | NM_001160016.1 | 90.1% | 100% | (many diffs) |
| 11 | mouse | 14688 | Gnb1 | guanine nucleotide binding ... | NM_001160017.1 | 90.1% | 100% | (many diffs) |
| 12 | mouse | 14688 | Gnb1 | guanine nucleotide binding ... | NM_008142.4 | 90.1% | 100% | (many diffs) |
| 13 | mouse | 14688 | Gnb1 | guanine nucleotide binding ... | XM_017319977.2 | 90.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgagcttgac cagttacggc aggaggccga gcaacttaag aaccagattc 121 gagacgccag gaaagcatgt gcagatgcaa ctctctctca gatcacaaac aacatcgacc 181 cagtgggaag aatccaaatg cgcacgagga ggacactgcg ggggcacctg gccaagatct 241 acgccatgca ctggggcaca gactccaggc ttctcgtcag tgcctcgcag gatggtaaac 301 ttatcatctg ggacagctac accaccaaca aggtccacgc catccctctg cgctcctcct 361 gggtcatgac ctgtgcatat gccccttctg ggaactatgt ggcctgcggt ggcctggata 421 acatttgctc catttacaat ctgaaaactc gtgaggggaa cgtgcgcgtg agtcgtgagc 481 tggcaggaca cacaggttac ctgtcctgct gccgattcct ggatgacaat cagatcgtca 541 ccagctctgg agacaccacg tgtgccctgt gggacatcga gaccggccag cagacgacca 601 cgtttaccgg acacactgga gatgtcatga gcctttctct tgctcctgac accagactgt 661 tcgtcTCTGG TGCTTGTGAT GCTTCAGCCA AACTCTGGGA TGTGCGAGAA GGCATGTGCC 721 GGCAGACCTT CACTGGCCAC GAGTCTGACA TCAATGCCAT TTGCTTCTTT CCAAATGGCA 781 ATGCATTTGC CACTGGCTCA GACGACGCCA CCTGCAGGCT GTTTGACCTT CGTGCTGACC 841 AGGAGCTCAT GACTTACTCC CATGACAACA TCATCTGCGG GATCACCTCT GTCTCCTTCT 901 CCAAGAGCGG GCGCCTCCTC CTTGCTGGGT ACGACGACTT CAACTGCAAC GTCTGGGATG 961 CACTCAAAGC CGACCGGGCA GGTGTCTTGG CTGGGCATGA CAACCGCGTC AGCTGCCTGG 1021 GCGTGACTGA CGATGGCATG GCTGTGGCGA CAGGGTCCTG GGATAGCTTC CTCAAGATCT 1081 GGAACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTGGTCGC GCACGCAAGC GGTAAACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt