Construct: ORF TRCN0000489864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019119.1_s317c1
- DNA Barcode:
- TTAGACTCGCTATCAGCCAGGGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPHA7 (2045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489864
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248669.3 | 99.8% | 91.7% | (many diffs) |
| 2 | human | 2045 | EPHA7 | EPH receptor A7 | NM_004440.4 | 99.4% | 91.2% | (many diffs) |
| 3 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010365.2 | 99.3% | 91.2% | (many diffs) |
| 4 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288629.2 | 98.9% | 90.7% | (many diffs) |
| 5 | human | 2045 | EPHA7 | EPH receptor A7 | XM_005248671.3 | 60.9% | 65.6% | (many diffs) |
| 6 | human | 2045 | EPHA7 | EPH receptor A7 | XM_017010366.2 | 44.4% | 48.3% | 981G>A;1324_1325insCTCCCTCGCAAGT;1326_1327ins1644 |
| 7 | human | 2045 | EPHA7 | EPH receptor A7 | XR_001743218.2 | 37.2% | (many diffs) | |
| 8 | human | 2045 | EPHA7 | EPH receptor A7 | NM_001288630.1 | 28% | 30.3% | 832_833insCCT;834_835insGGCCGTGGGTTCTAC;837_838ins2128 |
| 9 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001290434.1 | 91.1% | 90.1% | (many diffs) |
| 10 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_010141.4 | 90.8% | 89.6% | (many diffs) |
| 11 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537606.3 | 90.3% | 89.1% | (many diffs) |
| 12 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_006537607.3 | 56.6% | 64.9% | (many diffs) |
| 13 | mouse | 13841 | Epha7 | Eph receptor A7 | NM_001122889.1 | 55.8% | 64.2% | (many diffs) |
| 14 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_017319954.1 | 55.3% | 63.6% | (many diffs) |
| 15 | mouse | 13841 | Epha7 | Eph receptor A7 | XM_017319955.1 | 48.8% | 56.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2808
- ORF length:
- 2736
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggttttt caaactcggt acccttcatg gattatttta tgctacatct 121 ggctgctccg ctttgcacac acaggggagg cgcaggctgc gaaggaagta ctactgctgg 181 attctaaagc acaacaaaca gagttggagt ggatttcctc tccacccaat gggtgggaag 241 aaattagtgg tttggatgag aactataccc cgatacgaac ataccaggtg tgccaagtca 301 tggagcccaa ccaaaacaac tggctgcgga ctaactggat ttccaaaggc aatgcacaaa 361 ggatttttgt agaattgaaa ttcaccctga gggattgtaa cagtcttcct ggagtactgg 421 gaacttgcaa ggaaacattt aatttgtact attatgaaac agactatgac actggcagga 481 atataagaga aaacctctat gtaaaaatag acaccattgc tgcagatgaa agttttaccc 541 aaggtgacct tggtgaaaga aagatgaagc ttaacactga ggtgagagag attggacctt 601 tgtccaaaaa gggattctat cttgcctttc aggatgtagg ggcttgcata gctttggttt 661 ctgtcaaagt gtactacaag aagtgctggt ccattattga gaacttagct atctttccag 721 atacagtgac tggttcagaa ttttcctctt tagtcgaggt tcgagggaca tgtgtcagca 781 gtgcagagga agaagcggaa aacgccccca ggatgcactg cagtgcagaa ggagaatggt 841 tagtgcccat tggaaaatgt atctgcaaag caggctacca gcaaaaagga gacacttgtg 901 aaccctgtgg ccgtgggttc tacaagtctt cctctcaaga tcttcagtgc tctcgttgtc 961 caactcacag tttttctgat aaagaaggct cctccagatg tgaatgtgaa gatgggtatt 1021 acagggctcc atctgaccca ccatacgttg catgcacaag gcctccatct gcaccacaga 1081 acctcatttt caacatcaac caaaccacag taagtttgga atggagtcct cctgcagaca 1141 atgggggaag aaacgatgtg acctacagaa tattgtgtaa gcggtgcagt tgggagcagg 1201 gcgaatgtgt tccctgtggg agtaacattg gatacatgcc ccagcagact ggattagagg 1261 ataactatgt cactgtcatg gacctgctag cccacgctaa ttatactttt gaagttgaag 1321 ctgtaaatgg agtttctgac ttaagccgat cccagaggct ctttgctgct gtcagtatca 1381 ccactggtca agcagctccc tcgcaagtga gcggagtaat gaaggagaga gtactgcagc 1441 ggagtgtcga gctttcctgg caggaaccag agcatcccaa tggagtcatc acagaatatg 1501 aaatcaagta ttacgagaaa gatcaaaggg aacggaccta ctcaacagta aaaaccaagt 1561 ctacttcagc ctccattaat aatctgaaac caggaacagt gtatgttttc cagattcggg 1621 cttttactgc tgctggttat ggaaattaca gtcccagact tgatgttgct acactagagg 1681 aagctacagg taaaatgttt gaagctacag ctgtctccag tgaacagaat cctgttatta 1741 tcattgctgt ggttgctgta gctgggacca tcattttggt gttcatggtc tttggcttca 1801 tcattgggag aaggcactgt ggttatagca aagctgacca agaaggcgat gaagagcttt 1861 actttcattg caccaaaacc tacattgacc ctgaaaccta tgaggaccca aatagagctg 1921 tccatcaatt cgccaaggag ctagatgcct cctgtattaa aattgagcgt gtgattggtg 1981 caggagaatt cggtgaagtc tgcagtggcc gtttgaaact tccagggaaa agagatgttg 2041 cagtagccat aaaaaccctg aaagttggtt acacagaaaa acaaaggaga gactttttgt 2101 gtgaagcaag catcatgggg cagtttgacc acccaaatgt tgtccatttg gaaggggttg 2161 ttacaagagg gaaaccagtc atgatagtaa tagagttcat ggaaaatgga gccctagatg 2221 catttctcag gaaacatgat gggcaattta cagtcattca gttagtagga atgctgagag 2281 gaattgctgc tggaatgaga tatttggctg atatgggata tgttcacagg gaccttgcag 2341 ctcgcaatat tcttgtcaac agcaatctcg tttgtaaagt gtcagatttt ggcctgtccc 2401 gagttataga ggatgatcca gaagctgtct atacaactac tggtggaaaa attccagtaa 2461 ggtggacagc acccgaagcc atccagtacc ggaaattcac atcagccagt gatgtatgga 2521 gctatggaat agtcatgtgg gaagttatgt cttatggaga aagaccttat tgggacatgt 2581 caaatcaaga tgttataaaa gcaatagaag aaggttatcg tttaccagca cccatggact 2641 gcccagctgg CCTTCACCAG CTAATGTTGG ATTGTTGGCA AAAGGAGCGT GCTGAAAGGC 2701 CAAAATTTGA ACAGATAGTT GGAATTCTAG ACAAAATGAT TCGAAACCCA AATAGTCTGA 2761 AAACTCCCCT GGGAACTTGT AGTAGGCCAA TAAGCCCTCT TCTGGATTAA AACACTCCTG 2821 ATTTCACTAC CTTTTGTTCA GTTGGAGAAT GGCTACAAGC TATTAAGATG GAAAGATATA 2881 AAGATAATTT CACGGCAGCT GGCTACAATT CCCTTGAATC AGTAGCCAGG ATGACTATTG 2941 AGGATGTGAT GAGTTTAGGG ATCACACTGG TTGGTCATCA AAAGAAAATC ATGAGCAGCA 3001 TTCAGACTAT GAGAGCACAA ATGCTACATT TACATGGAAC TGGCATTCAA GTGGACCCAG 3061 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3121 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3181 TATCTTGTGG AAAGGACGAT TAGACTCGCT ATCAGCCAGG GCCACGCGTT AAGTCgacaa 3241 tcaacctctg gattacaaaa tttgtgaaag att