Transcript: Mouse NM_001291816.1

Mus musculus cyclin-dependent kinase 19 (Cdk19), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Cdk19 (78334)
Length:
5752
CDS:
571..1716

Additional Resources:

NCBI RefSeq record:
NM_001291816.1
NBCI Gene record:
Cdk19 (78334)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001291816.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360759 CTGTCGTCAGGAGGATATAAA pLKO_005 915 CDS 100% 15.000 21.000 N Cdk19 n/a
2 TRCN0000360758 TGATCAGTTAGATCGAATATT pLKO_005 957 CDS 100% 15.000 21.000 N Cdk19 n/a
3 TRCN0000360761 CCCGTTATGCCGTCGGATTAT pLKO_005 1564 CDS 100% 13.200 18.480 N Cdk19 n/a
4 TRCN0000360760 TTACCAAGATCCATGGTTAAA pLKO_005 577 CDS 100% 13.200 18.480 N Cdk19 n/a
5 TRCN0000023280 GCACCCTAATGTGATCGCATT pLKO.1 432 5UTR 100% 4.050 5.670 N Cdk19 n/a
6 TRCN0000023281 CGTCGGATTATCAGCATTCCA pLKO.1 1574 CDS 100% 3.000 4.200 N Cdk19 n/a
7 TRCN0000003143 ACCAGCAAATATCCTAGTAAT pLKO.1 669 CDS 100% 13.200 10.560 N CDK19 n/a
8 TRCN0000023283 CCCAACACTTCAGAAAGACTT pLKO.1 1038 CDS 100% 4.950 3.465 N Cdk19 n/a
9 TRCN0000023279 CCTGCCAACATTAGATGTGTT pLKO.1 1227 CDS 100% 4.950 3.465 N Cdk19 n/a
10 TRCN0000023282 CTGCTGTTTGACTATGCAGAA pLKO.1 493 5UTR 100% 4.050 2.835 N Cdk19 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001291816.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489647 ACTGCTTCTTCCAGGGACTCAATC pLX_317 27.9% 67.3% 72.5% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_15005 pDONR223 100% 62.5% 3% None (many diffs) n/a
3 ccsbBroad304_15005 pLX_304 0% 62.5% 3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000470685 TTCAGCCAGAAATAAAATGGCCAT pLX_317 25.5% 62.5% 3% V5 (not translated due to prior stop codon) (many diffs) n/a
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