Transcript: Human NM_001321266.2

Homo sapiens membrane bound O-acyltransferase domain containing 2 (MBOAT2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
MBOAT2 (129642)
Length:
7398
CDS:
246..1409

Additional Resources:

NCBI RefSeq record:
NM_001321266.2
NBCI Gene record:
MBOAT2 (129642)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321266.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000370550 TTTACAGCTCCTGGTATTATT pLKO_005 1177 CDS 100% 15.000 12.000 N MBOAT2 n/a
2 TRCN0000217116 CAGGCCCAATGATGATCATTA pLKO.1 238 5UTR 100% 13.200 9.240 N Mboat2 n/a
3 TRCN0000248024 CAGGCCCAATGATGATCATTA pLKO_005 238 5UTR 100% 13.200 9.240 N Mboat2 n/a
4 TRCN0000370670 CAGGCCCAATGATGATCATTA pLKO_005 238 5UTR 100% 13.200 9.240 N MBOAT2 n/a
5 TRCN0000303284 CTTGCCTTAAACCCTCTATTT pLKO_005 1786 3UTR 100% 13.200 9.240 N MBOAT2 n/a
6 TRCN0000035240 GCTCTTACAAAGACTACATTA pLKO.1 424 CDS 100% 13.200 9.240 N MBOAT2 n/a
7 TRCN0000291673 GCTCTTACAAAGACTACATTA pLKO_005 424 CDS 100% 13.200 9.240 N MBOAT2 n/a
8 TRCN0000303283 TTGACCATCTGTACAACATTA pLKO_005 588 CDS 100% 13.200 9.240 N MBOAT2 n/a
9 TRCN0000035242 CCAGGATATTATCTAACGTTT pLKO.1 975 CDS 100% 4.950 3.465 N MBOAT2 n/a
10 TRCN0000035239 CGAGTCTATATCTTTGACTAT pLKO.1 195 5UTR 100% 4.950 3.465 N MBOAT2 n/a
11 TRCN0000175588 CCAAATGAATGTCTTGCCTTA pLKO.1 1774 3UTR 100% 4.050 2.835 N Mboat2 n/a
12 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 3292 3UTR 100% 4.050 2.025 Y P3H4 n/a
13 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 3292 3UTR 100% 4.050 2.025 Y ORAI2 n/a
14 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 3292 3UTR 100% 4.050 2.025 Y P3H4 n/a
15 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 3419 3UTR 100% 10.800 5.400 Y SMIM11A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321266.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14373 pDONR223 100% 90.3% 90.1% None 1_111del;785C>A n/a
2 ccsbBroad304_14373 pLX_304 0% 90.3% 90.1% V5 1_111del;785C>A n/a
3 TRCN0000474282 TAATTGTGAAAGGAATTACCCCAA pLX_317 42.7% 90.3% 90.1% V5 1_111del;785C>A n/a
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