Construct: ORF TRCN0000474396
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018844.3_s317c1
- Derived from:
- ccsbBroadEn_14562
- DNA Barcode:
- TAATTGTTATTCACCCAAGAGACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CAMK2A (815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474396
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001363990.1 | 99.7% | 47.9% | (many diffs) |
| 2 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_171825.2 | 99.7% | 47.9% | (many diffs) |
| 3 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001369025.2 | 99.6% | 47.9% | (many diffs) |
| 4 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001363989.1 | 97.4% | 46.8% | (many diffs) |
| 5 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_015981.4 | 97.4% | 46.8% | (many diffs) |
| 6 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172083.3 | 79.9% | 43.4% | (many diffs) |
| 7 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249864.3 | 77.6% | 42.1% | (many diffs) |
| 8 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012665.1 | 77.5% | 42.1% | (many diffs) |
| 9 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172081.3 | 76.1% | 41.3% | (many diffs) |
| 10 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012664.1 | 76% | 41.4% | (many diffs) |
| 11 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172084.3 | 75.3% | 46.3% | (many diffs) |
| 12 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715784.2 | 74.2% | 40.1% | (many diffs) |
| 13 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172079.2 | 74.1% | 40.1% | (many diffs) |
| 14 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_006714330.3 | 74% | 44.9% | (many diffs) |
| 15 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172080.3 | 74% | 40.2% | (many diffs) |
| 16 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_172128.3 | 73.4% | 43.9% | (many diffs) |
| 17 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321576.2 | 73.3% | 44% | (many diffs) |
| 18 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008678.2 | 73.1% | 44% | (many diffs) |
| 19 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249862.3 | 72.9% | 39.4% | (many diffs) |
| 20 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321582.2 | 72.4% | 42.9% | (many diffs) |
| 21 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321575.2 | 72.4% | 43.6% | (many diffs) |
| 22 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321577.2 | 72.3% | 43.7% | (many diffs) |
| 23 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532291.2 | 72.2% | 42.9% | (many diffs) |
| 24 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321583.2 | 72.2% | 43.6% | (many diffs) |
| 25 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008677.2 | 72.1% | 43.7% | (many diffs) |
| 26 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321590.2 | 71.6% | 42.6% | (many diffs) |
| 27 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321584.2 | 71.5% | 42.7% | (many diffs) |
| 28 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321568.2 | 71.5% | 42.7% | (many diffs) |
| 29 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321589.2 | 71.3% | 42.6% | (many diffs) |
| 30 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001293170.2 | 71% | 38.3% | (many diffs) |
| 31 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172078.3 | 71% | 38.3% | (many diffs) |
| 32 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321587.2 | 70.6% | 41.7% | (many diffs) |
| 33 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172082.3 | 70.5% | 42.2% | (many diffs) |
| 34 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001221.4 | 70.4% | 42% | (many diffs) |
| 35 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321591.2 | 70.4% | 41.7% | (many diffs) |
| 36 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321574.2 | 70.4% | 42.1% | (many diffs) |
| 37 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532295.2 | 70.4% | 42.1% | (many diffs) |
| 38 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321572.2 | 70.1% | 41.9% | (many diffs) |
| 39 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008674.2 | 70.1% | 42% | (many diffs) |
| 40 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321567.2 | 69.9% | 41.9% | (many diffs) |
| 41 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532289.2 | 69.5% | 41.1% | (many diffs) |
| 42 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008672.2 | 69.5% | 41.9% | (many diffs) |
| 43 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_011532290.2 | 69.5% | 41.2% | (many diffs) |
| 44 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_017008673.2 | 69.5% | 41.9% | (many diffs) |
| 45 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321570.2 | 69.3% | 41% | (many diffs) |
| 46 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321586.2 | 69.2% | 41% | (many diffs) |
| 47 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321579.2 | 69.1% | 41% | (many diffs) |
| 48 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321566.2 | 68.7% | 40.9% | (many diffs) |
| 49 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321588.2 | 68.7% | 41% | (many diffs) |
| 50 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321580.2 | 68.4% | 40.4% | (many diffs) |
| 51 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321573.2 | 67.9% | 40% | (many diffs) |
| 52 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321571.2 | 67.4% | 40.3% | (many diffs) |
| 53 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_005263254.4 | 67.4% | 40.4% | (many diffs) |
| 54 | human | 817 | CAMK2D | calcium/calmodulin dependen... | NM_001321569.2 | 66.7% | 39.3% | (many diffs) |
| 55 | human | 817 | CAMK2D | calcium/calmodulin dependen... | XM_005263253.4 | 66.6% | 39.4% | (many diffs) |
| 56 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012662.1 | 63.7% | 34.4% | (many diffs) |
| 57 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_024446945.1 | 61.5% | 33.1% | (many diffs) |
| 58 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715781.2 | 61.2% | 33.2% | (many diffs) |
| 59 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012661.1 | 58.7% | 31.7% | (many diffs) |
| 60 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515556.1 | 58.2% | 31.4% | (many diffs) |
| 61 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515557.1 | 58.2% | 31.5% | (many diffs) |
| 62 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001220.5 | 58% | 31.2% | (many diffs) |
| 63 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515555.1 | 58% | 31.2% | (many diffs) |
| 64 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515553.1 | 57.6% | 30.9% | (many diffs) |
| 65 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012660.1 | 57.4% | 31% | (many diffs) |
| 66 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515554.1 | 57.3% | 31% | (many diffs) |
| 67 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515551.1 | 56.4% | 30.3% | (many diffs) |
| 68 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515550.1 | 56.3% | 30.3% | (many diffs) |
| 69 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515552.1 | 56.2% | 30.4% | (many diffs) |
| 70 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515549.1 | 55.5% | 29.9% | (many diffs) |
| 71 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012670.2 | 54.7% | 18.7% | (many diffs) |
| 72 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515547.1 | 54.5% | 29.3% | (many diffs) |
| 73 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012669.2 | 53.5% | 18.1% | (many diffs) |
| 74 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012668.2 | 53.3% | 18.1% | (many diffs) |
| 75 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_024446946.1 | 53.2% | 18.2% | (many diffs) |
| 76 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012667.2 | 52.4% | 17.8% | (many diffs) |
| 77 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012666.2 | 51.2% | 17.3% | (many diffs) |
| 78 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012663.2 | 40.6% | 13.7% | (many diffs) |
| 79 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515559.2 | 39.3% | 13.2% | (many diffs) |
| 80 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_177407.4 | 92.8% | 47.9% | (many diffs) |
| 81 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | XM_017317805.2 | 92.8% | 47.9% | (many diffs) |
| 82 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | XM_006525541.4 | 90.8% | 46.8% | (many diffs) |
| 83 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514479.2 | 76.2% | 41.3% | (many diffs) |
| 84 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514476.1 | 74% | 40.1% | (many diffs) |
| 85 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514477.1 | 74% | 40.2% | (many diffs) |
| 86 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514475.1 | 73% | 39.4% | (many diffs) |
| 87 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314240.1 | 72.9% | 39.3% | (many diffs) |
| 88 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174054.1 | 72.5% | 39.3% | (many diffs) |
| 89 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_007595.5 | 71% | 38.3% | (many diffs) |
| 90 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314239.1 | 70.9% | 38.3% | (many diffs) |
| 91 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174053.1 | 70.3% | 38.1% | (many diffs) |
| 92 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514469.2 | 61.4% | 33.1% | (many diffs) |
| 93 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514470.3 | 61.3% | 33.2% | (many diffs) |
| 94 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514467.2 | 60% | 32.3% | (many diffs) |
| 95 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314238.1 | 59.9% | 32.4% | (many diffs) |
| 96 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314237.1 | 59.2% | 31.9% | (many diffs) |
| 97 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514465.2 | 58% | 31.2% | (many diffs) |
| 98 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514464.2 | 57.9% | 31.1% | (many diffs) |
| 99 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_001286809.1 | 35.8% | 14.3% | (many diffs) |
| 100 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_009792.3 | 35% | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 759
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaccatc acctgcaccc gcttcacgga agagtaccag ctcttcgagg 121 aattgggcaa gggagccttc tcggtggtgc gaaggtgtgt gaaggtgctg gctggccagg 181 agtatgctgc caagatcatc aacacaaaga agctgtcagc cagagaccat cagaagctgg 241 agcgtgaagc ccgcatctgc cgcctgctga agcaccccaa catcgtccga ctacatgaca 301 gcatctcaga ggagggacac cactacctga tcttcgacct ggtcactggt ggggaactgt 361 ttgaagatat cgtggcccgg gagtattaca gtgaggcgga tgccagtcac tgtatccagc 421 agatcctgga ggctgtgctg cactgccacc agatgggggt ggtgcaccgg gacctgaagc 481 ctgagaatct gttgctggcc tccaagctca agggtgccgc agtgaagctg gcagactttg 541 gcctggccat agaggtggag ggggagcagc aggcatggtt tgggtttgca gggactcctg 601 gatatctctc cccagaagtg ctgcggaagg acccgtacgg gaagcctgtg gacctgtggg 661 cttgtggggt catcctgtac atcctgctgg ttgggtaccc cccgttctgg gatgaggacc 721 agcaccgcct gtaccagcag atcaaagccg gcgccttatg atttcccatc gccggaatgg 781 gacactgtca ccccggaagc caaggatctg atcaataaga tgctgaccat taacccatcc 841 aaacgcatca cagctgccga agccattaag cacccctgga tctcgcaccg ctccaccgtg 901 gcatcgtgca tgcacagaca ggagaccgtg gactgcctga agaagttcaa tgccaggagg 961 aaactgaagg gagccattct caccacgatg ctggccacca ggaacttctc cggagggaag 1021 agtgggggaa acaagaagag cgatggtgtg aaggaatcct cagagagcac caacaccacc 1081 atcgaggatg aagacaccaa agtgcggaaa caggaaatta taaaagtgac agagcagctg 1141 attgaagcca taagcaatgg agatttTGAG TCCTACACGA AGATGTGCGA CCCTGGCATG 1201 ACAGCCTTCG AACCTGAGGC CCTGGGGAAC CTGGTTGAGG GCCTGGACTT CCATCGATTC 1261 TATTTTGAAA ACCTGTGGTC CCGGAACAGC AAGCCCGTGC ACACCACCAT CCTGAATCCC 1321 CACATCCACC TGATGGGCGA CGAGTCAGCC TGCATCGCCT ACATCCGCAT CACGCAGTAC 1381 CTGGACGCTG GCGGCATCCC ACGCACCGCC CAGTCGGAGG AGACCCGTGT CTGGCACCGC 1441 CGGGACGGCA AATGGCAGAT CGTCCACTTC CACAGATCTG GGGCGCCCTC CGTCCTGCCC 1501 CACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 1561 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 1621 TGGCTTTATA TATCTTGTGG AAAGGACGAT AATTGTTATT CACCCAAGAG ACTACGCGTT 1681 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att