Transcript: Human NM_001330301.2

Homo sapiens Sin3A associated protein 130 (SAP130), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
SAP130 (79595)
Length:
4084
CDS:
127..3300

Additional Resources:

NCBI RefSeq record:
NM_001330301.2
NBCI Gene record:
SAP130 (79595)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330301.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130598 CCAAGCATACTCCGGAAGAAA pLKO.1 2050 CDS 100% 5.625 7.875 N SAP130 n/a
2 TRCN0000352903 CCAAGCATACTCCGGAAGAAA pLKO_005 2050 CDS 100% 5.625 7.875 N SAP130 n/a
3 TRCN0000130267 CCGACCATTCCAACTATGATT pLKO.1 2317 CDS 100% 5.625 7.875 N SAP130 n/a
4 TRCN0000343352 CCGACCATTCCAACTATGATT pLKO_005 2317 CDS 100% 5.625 7.875 N SAP130 n/a
5 TRCN0000129249 CAGGTGGAAATGAGTTCTCAA pLKO.1 118 5UTR 100% 4.950 3.465 N SAP130 n/a
6 TRCN0000343351 CAGGTGGAAATGAGTTCTCAA pLKO_005 118 5UTR 100% 4.950 3.465 N SAP130 n/a
7 TRCN0000129185 CCACCGAATAAACGAACTGAT pLKO.1 3117 CDS 100% 4.950 3.465 N SAP130 n/a
8 TRCN0000352838 CCACCGAATAAACGAACTGAT pLKO_005 3117 CDS 100% 4.950 3.465 N SAP130 n/a
9 TRCN0000128878 GCTGTGATGTCCAGTTCTAAA pLKO.1 760 CDS 100% 13.200 7.920 N SAP130 n/a
10 TRCN0000352837 GCTGTGATGTCCAGTTCTAAA pLKO_005 760 CDS 100% 13.200 7.920 N SAP130 n/a
11 TRCN0000217226 CCAGTAACCTGCATCACATTA pLKO.1 641 CDS 100% 13.200 9.240 N Sap130 n/a
12 TRCN0000248218 GTGGCCATGGAAACCCGAAAT pLKO_005 1453 CDS 100% 10.800 7.560 N Sap130 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330301.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12570 pDONR223 100% 64.9% 64.9% None 1_1008del;1957_2061del n/a
2 ccsbBroad304_12570 pLX_304 0% 64.9% 64.9% V5 1_1008del;1957_2061del n/a
3 TRCN0000474581 CGAGACCTTGCACACCGGTTTCAC pLX_317 25.4% 64.9% 64.9% V5 1_1008del;1957_2061del n/a
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