Construct: ORF TRCN0000474581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018276.1_s317c1
- Derived from:
- ccsbBroadEn_12570
- DNA Barcode:
- CGAGACCTTGCACACCGGTTTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAP130 (79595)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_001330303.2 | 67.1% | 67.1% | 1_1008del |
| 2 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_001330300.2 | 67% | 67% | 1_1008del;1777_1778insCAG |
| 3 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_024545.4 | 65.4% | 65.4% | 1_1086del |
| 4 | human | 79595 | SAP130 | Sin3A associated protein 130 | XM_006712749.3 | 65.3% | 65.3% | 1_1086del;1855_1856insCAG |
| 5 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_001330299.2 | 64.9% | 64.9% | 1_1005del;1954_2058del |
| 6 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_001330301.2 | 64.9% | 64.9% | 1_1008del;1957_2061del |
| 7 | human | 79595 | SAP130 | Sin3A associated protein 130 | XM_005263769.3 | 64.9% | 64.9% | 1_1008del;1957_2061del |
| 8 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_001330302.2 | 64.8% | 64.8% | 1_1008del;1777_1778insCAG;1954_2058del |
| 9 | human | 79595 | SAP130 | Sin3A associated protein 130 | XM_005263767.3 | 63.4% | 63.4% | 1_1083del;2032_2136del |
| 10 | human | 79595 | SAP130 | Sin3A associated protein 130 | NM_001145928.1 | 63.3% | 63.3% | 1_1086del;2035_2139del |
| 11 | human | 79595 | SAP130 | Sin3A associated protein 130 | XM_006712748.3 | 63.2% | 63.2% | 1_1086del;1855_1856insCAG;2032_2136del |
| 12 | human | 79595 | SAP130 | Sin3A associated protein 130 | XR_923026.3 | 45.1% | (many diffs) | |
| 13 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_017317912.1 | 58.3% | 62.4% | (many diffs) |
| 14 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_006525953.1 | 58% | 62% | (many diffs) |
| 15 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_006525951.2 | 57.2% | 61.2% | (many diffs) |
| 16 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_006525952.3 | 57.2% | 61.2% | (many diffs) |
| 17 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_006525954.3 | 57.2% | 61.2% | (many diffs) |
| 18 | mouse | 269003 | Sap130 | Sin3A associated protein | NM_172965.2 | 57.1% | 61.1% | (many diffs) |
| 19 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_006525949.1 | 56.1% | 60% | (many diffs) |
| 20 | mouse | 269003 | Sap130 | Sin3A associated protein | XM_006525950.2 | 56% | 59.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2124
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcagaaaaca atcttcagta ctggcacgcc agtggctgca gccacagtag 121 cacctatttt ggcaaccaac accattcctt cagcgaccac agctggatct gtgtcacaca 181 cgcaagctcc cacaagtacc attgttacca tgacagtacc ctcccattcc tcccatgcta 241 ctgctgtgac cacctcaaac atcccagtcg ccaaggtggt gccccagcag atcacgcaca 301 cttctcctcg gatccagcca gactaccctg ccgagaggag tagcctgatt cccatctccg 361 gacatcgggc ctctcccaat cctgtggcca tggaaacccg aagtgacaac agaccgtctg 421 ttcccgttca gttccaatat tttttgccaa cttacccccc ttctgcatac ccactggcgg 481 cacataccta caccccaatc accagttccg tgtccactat ccgacagtat ccagtttcag 541 ctcaggctcc aaactctgcc atcacagctc agactggtgt tggggtagcg tctaccgtcc 601 acctaaaccc catgcagttg atgacagtgg atgcatcgca tgctcgacat attcaaggga 661 tccagccagc acccatcagt acccagggta tccagccggc ccccattggg accccaggga 721 tacagcctgc accacttggc acacagggaa ttcactcagc aaccccaatc aacacacaag 781 ggcttcagcc tgcacctatg ggtactcagc agcctcagcc tgaaggaaag acttcagcag 841 tggtgttggc agatggagcc acaattgtgg ccaaccctat tagcaatcca ttcagtgctg 901 ctccagcagc aacaaccgtg gtgcagaccc acagccagag tgctagcacc aacgctcccg 961 cccagggctc atcgccacgg ccaagcatac tccggaagaa acctgccaca gatggtgcca 1021 aacccaagtc tgaaatccac gtgtctatgg ccactccggt cactgtgtcc atggagactg 1081 tatccaatca aaataatgat cagcctacca ttgccgtccc tccaactgcc cagcagcccc 1141 caccgaccat tccaactatg attgcagcag ccagtccccc gtcacaacca gccgttgccc 1201 tttcaaccat tcctggagcg gtccccatca ctccacccat caccaccatt gcagctgcac 1261 cacctccatc agtcactgtg ggtggcagtc tttcctccgt cttgggccct cccgttcctg 1321 aaattaaagt gaaagaagaa gtagaaccaa tggatatcat gaggccagtt tctgcagttc 1381 ctccactggc taccaacact gtgtctccat ctcttgcatt gctggcaaac aacttgtcca 1441 tgcctacaag tgacctacca cctggtgcct ccccaaggaa aaagcctcga aagcaacagc 1501 atgtgatctc aacagaagaa ggtgacatga tggagacaaa cagcactgat gatgagaagt 1561 ccactgccaa gagtcttctg gtgaaggctg agaagcgcaa gtctccTCCC AAGGAGTATA 1621 TTGATGAGGA AGGTGTGAGA TATGTCCCAG TGCGTCCAAG ACCCCCCATT ACTTTGCTTC 1681 GTCACTATCG GAACCCCTGG AAAGCTGCTT ACCACCACTT TCAGAGGTAC AGTGACGTCC 1741 GGGTCAAAGA GGAGAAGAAA GCTATGCTGC AGGAAATAGC TAATCAGAAA GGAGTATCCT 1801 GTCGTGCTCA AGGCTGGAAA GTCCACCTCT GTGCTGCCCA GTTACTACAG CTGACGAATC 1861 TAGAACATGA TGTCTATGAA AGACTTACTA ACCTGCAGGA AGGGATTATC CCAAAGAAAA 1921 AAGCAGCAAC AGATGATGAT CTCCACCGAA TAAACGAACT GATACAGGGA AATATGCAGA 1981 GGTGTAAACT TGTGATGGAT CAAATCAGTG AAGCCAGAGA CTCCATGCTT AAGGTTTTAG 2041 ATCATAAAGA CCGTGTCCTG AAGCTGCTTA ACAAGAACGG GACTGTCAAA AAAGTGTCCA 2101 AATTGAAGCG AAAGGAAAAA GTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2161 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2221 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GAGACCTTGC 2281 ACACCGGTTT CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2341 att