Transcript: Human NM_001347901.2

Homo sapiens ankyrin repeat domain 13D (ANKRD13D), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
ANKRD13D (338692)
Length:
2265
CDS:
557..2113

Additional Resources:

NCBI RefSeq record:
NM_001347901.2
NBCI Gene record:
ANKRD13D (338692)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001347901.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000166518 CCGGAACTGCTCAACAAACTT pLKO.1 620 CDS 100% 5.625 7.875 N ANKRD13D n/a
2 TRCN0000166761 CCAGCAAAGTACAGAGGTTCA pLKO.1 1353 CDS 100% 4.050 5.670 N ANKRD13D n/a
3 TRCN0000165070 GTCCAGCAAAGTACAGAGGTT pLKO.1 1351 CDS 100% 2.640 3.696 N ANKRD13D n/a
4 TRCN0000164831 CATTCCGGAACTGCTCAACAA pLKO.1 616 CDS 100% 4.950 3.465 N ANKRD13D n/a
5 TRCN0000162051 CGATTTCTACGTTGAGATGAA pLKO.1 652 CDS 100% 4.950 3.465 N ANKRD13D n/a
6 TRCN0000162319 CTGAGAAGATGGAAACTGTTA pLKO.1 1056 CDS 100% 4.950 3.465 N ANKRD13D n/a
7 TRCN0000166452 CGTTGAGATGAAGTGGGAGTT pLKO.1 661 CDS 100% 4.050 2.835 N ANKRD13D n/a
8 TRCN0000165930 GACACTCGTAATGTGGCCTTT pLKO.1 1001 CDS 100% 4.050 2.835 N ANKRD13D n/a
9 TRCN0000165095 GAAGACTCCATTCCAGTCCTT pLKO.1 1180 CDS 100% 2.640 1.848 N ANKRD13D n/a
10 TRCN0000165836 GATGGAAACTGTTAGCGGCTA pLKO.1 1063 CDS 100% 2.160 1.512 N ANKRD13D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001347901.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16158 pDONR223 0% 99.9% 100% None 1077T>C n/a
2 ccsbBroad304_16158 pLX_304 0% 99.9% 100% V5 1077T>C n/a
3 ccsbBroadEn_14474 pDONR223 100% 99.6% 99.4% None (many diffs) n/a
4 ccsbBroad304_14474 pLX_304 0% 99.6% 99.4% V5 (many diffs) n/a
5 TRCN0000476082 TCCGCTAAGCCGGGTGGCATAATT pLX_317 23.2% 99.6% 99.4% V5 (many diffs) n/a
6 ccsbBroadEn_16157 pDONR223 0% 47.9% 48.1% None (many diffs) n/a
7 ccsbBroad304_16157 pLX_304 0% 47.9% 48.1% V5 (many diffs) n/a
8 TRCN0000465531 TGACTCCTCTATTCTTTGGCACAT pLX_317 47.1% 47.9% 48.1% V5 (many diffs) n/a
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