Transcript: Human NM_001366043.1

Homo sapiens abhydrolase domain containing 18 (ABHD18), transcript variant 14, mRNA.

Source:
NCBI, updated 2019-03-22
Taxon:
Homo sapiens (human)
Gene:
ABHD18 (80167)
Length:
2665
CDS:
219..1565

Additional Resources:

NCBI RefSeq record:
NM_001366043.1
NBCI Gene record:
ABHD18 (80167)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001366043.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263935 GCCAAAGAAGATGCCTATATT pLKO_005 1434 CDS 100% 15.000 21.000 N ABHD18 n/a
2 TRCN0000263932 GAACTAAGCATGATGCATATT pLKO_005 1755 3UTR 100% 13.200 18.480 N ABHD18 n/a
3 TRCN0000129188 CGAACAGGAGTTCGAAGTTTA pLKO.1 1458 CDS 100% 1.320 1.848 N ABHD18 n/a
4 TRCN0000263933 TCATACCACCTACTTAGTAAA pLKO_005 1290 CDS 100% 13.200 10.560 N ABHD18 n/a
5 TRCN0000263931 GACAAGCTAACTAACCTTAAT pLKO_005 1074 CDS 100% 13.200 9.240 N ABHD18 n/a
6 TRCN0000149927 GCCTGAACATCTGTGGAATAA pLKO.1 1922 3UTR 100% 13.200 9.240 N ABHD18 n/a
7 TRCN0000263934 TGGAACAGGAGATCATCATTA pLKO_005 560 CDS 100% 13.200 9.240 N ABHD18 n/a
8 TRCN0000147139 CAGACAAGCTAACTAACCTTA pLKO.1 1072 CDS 100% 4.950 3.465 N ABHD18 n/a
9 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2081 3UTR 100% 4.950 2.475 Y ERAP2 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2082 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001366043.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12675 pDONR223 100% 73.2% 72.3% None (many diffs) n/a
2 ccsbBroad304_12675 pLX_304 0% 73.2% 72.3% V5 (many diffs) n/a
3 TRCN0000475662 CCTCAGTGAGCCAAGCCACAAATC pLX_317 28.2% 73.2% 72.3% V5 (many diffs) n/a
4 ccsbBroadEn_12676 pDONR223 100% 71.6% 71.6% None 1_381del n/a
5 ccsbBroad304_12676 pLX_304 0% 71.6% 71.6% V5 1_381del n/a
6 TRCN0000480245 AGGGTAGTTCTAAACACTCTTCTT pLX_317 29.2% 71.6% 71.6% V5 1_381del n/a
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