Construct: ORF TRCN0000475662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002397.1_s317c1
- Derived from:
- ccsbBroadEn_12675
- DNA Barcode:
- CCTCAGTGAGCCAAGCCACAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABHD18 (80167)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475662
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366048.1 | 100% | 100% | |
| 2 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_011532278.3 | 95.3% | 95.4% | 996_1044delinsG |
| 3 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001319305.2 | 86.8% | 86.9% | 454_555del;1098_1146delinsG |
| 4 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366041.2 | 82.3% | 82.4% | 0_1ins135;861_909delinsG |
| 5 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001039717.2 | 79.2% | 78.2% | (many diffs) |
| 6 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366044.1 | 78.4% | 78.4% | 0_1ins135;319_420del |
| 7 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366045.1 | 78.4% | 78.4% | 0_1ins135;319_420del |
| 8 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366047.1 | 78.4% | 78.4% | 0_1ins135;319_420del |
| 9 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_011532276.2 | 77.3% | 77.3% | 1_291del |
| 10 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366039.2 | 76.1% | 75.3% | (many diffs) |
| 11 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001319306.2 | 75% | 75.1% | 0_1ins135;319_420del;963_1011delinsG |
| 12 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_006714322.3 | 75% | 75.1% | 0_1ins135;319_420del;963_1011delinsG |
| 13 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_011532274.3 | 74.5% | 74.6% | 1_291del;1287_1335delinsG |
| 14 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366043.1 | 73.2% | 72.3% | (many diffs) |
| 15 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001358451.2 | 70.6% | 69.8% | (many diffs) |
| 16 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_024454227.1 | 70.6% | 69.8% | (many diffs) |
| 17 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366038.2 | 70.5% | 64.9% | (many diffs) |
| 18 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366037.2 | 70.3% | 70.4% | 0_1ins261;735_783delinsG |
| 19 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001358454.2 | 69.2% | 69.3% | 1_291del;745_846del;1389_1437delinsG |
| 20 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_006714320.3 | 69.2% | 69.3% | 1_291del;745_846del;1389_1437delinsG |
| 21 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_024454226.1 | 69.2% | 69.3% | 1_291del;745_846del;1389_1437delinsG |
| 22 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366046.1 | 66.9% | 66.9% | 0_1ins261;193_294del |
| 23 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001319307.2 | 64% | 64.1% | 0_1ins261;193_294del;837_885delinsG |
| 24 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366040.2 | 64% | 64.1% | 0_1ins261;193_294del;837_885delinsG |
| 25 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_001366042.2 | 64% | 64.1% | 0_1ins261;193_294del;837_885delinsG |
| 26 | human | 80167 | ABHD18 | abhydrolase domain containi... | XM_011532279.2 | 64% | 64.1% | 0_1ins261;193_294del;837_885delinsG |
| 27 | human | 80167 | ABHD18 | abhydrolase domain containi... | NM_025097.2 | 57.6% | 57.6% | 0_1ins363;91_192del |
| 28 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | XM_006500779.3 | 74.2% | 73.9% | (many diffs) |
| 29 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | XM_006500780.3 | 74.2% | 73.9% | (many diffs) |
| 30 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | NM_026622.3 | 62.1% | 62% | (many diffs) |
| 31 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | XM_017319591.1 | 62.1% | 62% | (many diffs) |
| 32 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | XM_006500781.2 | 56.2% | 54.9% | (many diffs) |
| 33 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | XM_017319592.1 | 56.2% | 54.9% | (many diffs) |
| 34 | mouse | 269423 | Abhd18 | abhydrolase domain containi... | XR_001783694.1 | 31.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1065
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttgatacc aacttgattc ttctaccttt gttccaattt attgtgccta 121 aagaatggaa cagcaaatat agacctgtat gcattcatct tgctggaaca ggagatcatc 181 attactggag gcgacgaaca ctaatggccc gtcctatgat taaagaagcc cgaatggctt 241 ctttattgtt agaaaaccct tattatggct gcaggaaacc caaggaccaa gtaaggtcca 301 gcttaaaaaa tgtgtccgac ctttttgtga tgggaggagc tcttgtttta gaatctgcag 361 ctctcttgca ctggctagag agggaaggtt acggcccttt aggaatgact ggaatatcca 421 tgggaggaca catggcttcc ttagcggtat ccaactggcc taagcccatg ccattgattc 481 catgcctgtc ttggtccaca gcatctgggg tcttcactac gacagattct ttcaaaatgg 541 gacaagagtt tgtgaaacac ttcactagca gtgcagacaa gctaactaac cttaatctgg 601 tttccagaac tttaaattta gatatatcaa accaagttgt atcccaaaaa cctgctgact 661 gccataattc tagcaaaaca tctgtcagtg cgacatcaga aggactctta ttgcaagata 721 cctctaagat gaagcgcttc aatcaaacac tttcaaccaa caaaagtggt tatacaagtc 781 gcaacccTCA GTCATACCAC CTACTTAGTA AAGAACAAAG CAGAAACAGT CTTCGGAAAG 841 AGTCTTTAAT ATTTATGAAA GGAGTCATGG ATGAATGTAC TCATGTAGCA AATTTCTCAG 901 TCCCAGTTGA TCCAAGCCTC ATTATAGTGG TTCAAGCCAA AGAAGATGCC TATATTCCAC 961 GAACAGGAGT TCGAAGTTTA CAAGAAATTT GGCCTGGTTG TGAAATCCGA TACTTAGAAG 1021 GGGGTCATAT TAGTGCTTAT CTTTTTAAAC AAGGACTCTT CAGGTTGCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA CCTCAGTGAG CCAAGCCACA AATCACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt