Transcript: Human NM_001371696.1

Homo sapiens cytochrome P450 family 20 subfamily A member 1 (CYP20A1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
CYP20A1 (57404)
Length:
10335
CDS:
135..1217

Additional Resources:

NCBI RefSeq record:
NM_001371696.1
NBCI Gene record:
CYP20A1 (57404)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001371696.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000271443 GATCGGTTTGATGATGAATTA pLKO_005 987 CDS 100% 13.200 18.480 N CYP20A1 n/a
2 TRCN0000064134 GCTGAAGTCATTATTAAGGTA pLKO.1 137 CDS 100% 3.000 4.200 N CYP20A1 n/a
3 TRCN0000284387 ATTATCCCTTCCCACTTATTT pLKO_005 4223 3UTR 100% 15.000 10.500 N CYP20A1 n/a
4 TRCN0000284388 AGGAACTTCAGTCAACATATT pLKO_005 561 CDS 100% 13.200 9.240 N CYP20A1 n/a
5 TRCN0000271445 TCTGTGGAGGGACAGGTTATT pLKO_005 1128 CDS 100% 13.200 9.240 N CYP20A1 n/a
6 TRCN0000271444 TTAGTACAAGGGAACCTTAAT pLKO_005 594 CDS 100% 13.200 9.240 N CYP20A1 n/a
7 TRCN0000064137 CTCATGCAACTGGAGTCTGTT pLKO.1 510 CDS 100% 4.950 3.465 N CYP20A1 n/a
8 TRCN0000064135 CCTAAAGCTTTCAGAAGAATT pLKO.1 254 CDS 100% 0.000 0.000 N CYP20A1 n/a
9 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 1888 3UTR 100% 4.950 2.475 Y CFLAR n/a
10 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 1888 3UTR 100% 4.950 2.475 Y C19orf31 n/a
11 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 7498 3UTR 100% 4.950 2.475 Y n/a
12 TRCN0000172586 GCCTGGCCAACATGATGAAAT pLKO.1 1819 3UTR 100% 13.200 6.600 Y SPIRE2 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1752 3UTR 100% 13.200 6.600 Y LIAS n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6493 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000162166 CAACATGATGAAACCCTGTAT pLKO.1 1826 3UTR 100% 4.950 2.475 Y SWSAP1 n/a
16 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 1886 3UTR 100% 4.950 2.475 Y ERN2 n/a
17 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 1886 3UTR 100% 4.950 2.475 Y P3H4 n/a
18 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 1886 3UTR 100% 4.950 2.475 Y P3H4 n/a
19 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 3248 3UTR 100% 4.950 2.475 Y DCAF11 n/a
20 TRCN0000164260 CCAACATGATGAAACCCTGTA pLKO.1 1825 3UTR 100% 4.050 2.025 Y SWSAP1 n/a
21 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6493 3UTR 100% 5.625 2.813 Y EID2B n/a
22 TRCN0000181017 GCACATGCCTTTAATCCCAAT pLKO.1 5372 3UTR 100% 4.050 2.025 Y Map6d1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001371696.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08724 pDONR223 100% 77.8% 77.7% None 0_1ins306;730C>T n/a
2 ccsbBroad304_08724 pLX_304 0% 77.8% 77.7% V5 0_1ins306;730C>T n/a
3 TRCN0000492151 TAACGACGCTCTCCTAGTGCACCA pLX_317 29.9% 77.8% 77.7% V5 0_1ins306;730C>T n/a
4 ccsbBroadEn_08725 pDONR223 100% 77.7% 77.4% None 0_1ins306;64A>G;730C>T n/a
5 ccsbBroad304_08725 pLX_304 0% 77.7% 77.4% V5 0_1ins306;64A>G;730C>T n/a
6 TRCN0000468493 TCAGATATGTATACACACTATTGT pLX_317 36.6% 77.7% 77.4% V5 0_1ins306;64A>G;730C>T n/a
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