Transcript: Human NM_005020.5

Homo sapiens phosphodiesterase 1C (PDE1C), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
PDE1C (5137)
Length:
8874
CDS:
234..2138

Additional Resources:

NCBI RefSeq record:
NM_005020.5
NBCI Gene record:
PDE1C (5137)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005020.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000435839 TCGAACCTTGGTAATTGAAAT pLKO_005 1220 CDS 100% 13.200 18.480 N PDE1C n/a
2 TRCN0000429736 ACTCGGTCTGATCCAGCTATT pLKO_005 1080 CDS 100% 10.800 15.120 N PDE1C n/a
3 TRCN0000048761 CCAGCTGTTATTGAGGCATTA pLKO.1 702 CDS 100% 10.800 15.120 N PDE1C n/a
4 TRCN0000048760 GCGTTCGAGTTTGAATAGCAT pLKO.1 1631 CDS 100% 3.000 4.200 N PDE1C n/a
5 TRCN0000114925 CCGAGAAGATTGTGAGTCCAT pLKO.1 1570 CDS 100% 2.640 3.696 N Pde1c n/a
6 TRCN0000048759 CGGTCTTTGGTCAAACAATTA pLKO.1 366 CDS 100% 13.200 10.560 N PDE1C n/a
7 TRCN0000433484 GAAGATTGTGAGTCCATTAAT pLKO_005 1574 CDS 100% 15.000 10.500 N PDE1C n/a
8 TRCN0000420303 GGGATCATGCACTGAAATTTA pLKO_005 775 CDS 100% 15.000 10.500 N PDE1C n/a
9 TRCN0000434501 TGTCAATGCCGTTACTTTAAA pLKO_005 2559 3UTR 100% 15.000 10.500 N PDE1C n/a
10 TRCN0000048762 CCGTTGACTATAAGAGCTTTA pLKO.1 1732 CDS 100% 10.800 7.560 N PDE1C n/a
11 TRCN0000417441 GCTTCAGTGGTAGATCTTAAG pLKO_005 399 CDS 100% 10.800 7.560 N PDE1C n/a
12 TRCN0000437003 TGACGGAGCTGGAGATCTTTG pLKO_005 991 CDS 100% 10.800 7.560 N PDE1C n/a
13 TRCN0000048758 CCCGATCAACAATTCTGTCAT pLKO.1 1709 CDS 100% 4.950 3.465 N PDE1C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005020.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489302 TGCATTTGGATGGTAACTCATCGC pLX_317 20.1% 99.8% 99.8% V5 (not translated due to prior stop codon) 510C>A;1621C>A;1773C>T n/a
2 ccsbBroadEn_06700 pDONR223 100% 99.7% 99.8% None (many diffs) n/a
3 ccsbBroad304_06700 pLX_304 0% 99.7% 99.8% V5 (many diffs) n/a
4 TRCN0000474141 CATGACTATCGAGCGACTGTGACG pLX_317 23.8% 99.7% 99.8% V5 (many diffs) n/a
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