Transcript: Human NM_006724.3

Homo sapiens mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
MAP3K4 (4216)
Length:
5414
CDS:
206..4882

Additional Resources:

NCBI RefSeq record:
NM_006724.3
NBCI Gene record:
MAP3K4 (4216)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148639 ACATAATCTGAACCCCACAT pXPR_003 GGG 1731 37% 4 0.7176 MAP3K4 MAP3K4 76510
2 BRDN0001148693 AGGACGGACAATTAACGACC pXPR_003 AGG 835 18% 3 0.623 MAP3K4 MAP3K4 76512
3 BRDN0001148603 ATAACTCATTACATACGGGG pXPR_003 AGG 2222 48% 6 0.3946 MAP3K4 MAP3K4 76511
4 BRDN0001146051 GCATGTCAACCAAACATCAG pXPR_003 AGG 297 6% 2 -0.5205 MAP3K4 MAP3K4 76513
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006724.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415053 CCTCAAGCATCGCATAGTTTA pLKO_005 2351 CDS 100% 13.200 18.480 N MAP3K4 n/a
2 TRCN0000010558 AGGTTAAAGAAGCTGCATGTT pLKO.1 5081 3UTR 100% 4.950 6.930 N MAP3K4 n/a
3 TRCN0000199102 CCGTGACATCAGCGTAGTGTT pLKO.1 5167 3UTR 100% 4.950 6.930 N MAP3K4 n/a
4 TRCN0000000849 CCGTGACATTAAAGGTGCCAA pLKO.1 4438 CDS 100% 2.640 3.696 N MAP3K4 n/a
5 TRCN0000417139 GCCAGCCAGTCGGTCTAATTT pLKO_005 523 CDS 100% 15.000 12.000 N MAP3K4 n/a
6 TRCN0000000851 GCCCGGAAGTGGATGAATTAT pLKO.1 3413 CDS 100% 15.000 12.000 N MAP3K4 n/a
7 TRCN0000194922 CCATCTCTCTTGAGTATTAAG pLKO.1 2132 CDS 100% 13.200 10.560 N MAP3K4 n/a
8 TRCN0000000852 CAACCAAACATCAGAGGAATA pLKO.1 492 CDS 100% 10.800 7.560 N MAP3K4 n/a
9 TRCN0000078953 GCTGCATGTTAAGTGCCATTA pLKO.1 5092 3UTR 100% 10.800 7.560 N LOC433718 n/a
10 TRCN0000196769 GCATGTTAAGTGCCATTACTA pLKO.1 5095 3UTR 100% 5.625 3.938 N MAP3K4 n/a
11 TRCN0000000850 GCTTCGCCTTTGTTAGAGATA pLKO.1 1125 CDS 100% 4.950 3.465 N MAP3K4 n/a
12 TRCN0000078956 GTCAAGGTTTGCACAGATGAA pLKO.1 4856 CDS 100% 4.950 3.465 N LOC433718 n/a
13 TRCN0000196922 GCAGTCCATTGAGGGACTTAT pLKO.1 3094 CDS 100% 1.320 0.924 N MAP3K4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006724.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.