Construct: ORF TRCN0000489251
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019066.1_s317c1
- DNA Barcode:
- TACACTTGTACATAAGTTAGCTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ULK2 (9706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489251
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9706 | ULK2 | unc-51 like autophagy activ... | NM_001142610.2 | 83% | 21.2% | (many diffs) |
| 2 | human | 9706 | ULK2 | unc-51 like autophagy activ... | NM_014683.4 | 83% | 21.2% | (many diffs) |
| 3 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025424.2 | 81.3% | 20.8% | (many diffs) |
| 4 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025425.2 | 81.3% | 20.8% | (many diffs) |
| 5 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025426.2 | 78.8% | 21.3% | (many diffs) |
| 6 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_011524087.2 | 71.6% | 9.8% | (many diffs) |
| 7 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XR_001752701.2 | 66.1% | (many diffs) | |
| 8 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025427.2 | 65.5% | 6.4% | (many diffs) |
| 9 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XM_017025428.2 | 62.8% | .8% | (many diffs) |
| 10 | human | 9706 | ULK2 | unc-51 like autophagy activ... | XR_001752700.2 | 43.9% | (many diffs) | |
| 11 | mouse | 29869 | Ulk2 | unc-51 like kinase 2 | NM_013881.4 | 74.9% | 20.9% | (many diffs) |
| 12 | mouse | 29869 | Ulk2 | unc-51 like kinase 2 | XM_006533508.3 | 73.7% | 20.6% | (many diffs) |
| 13 | mouse | 29869 | Ulk2 | unc-51 like kinase 2 | XM_006533509.3 | 73.1% | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 756
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggtg gtgggtgact tcgagtacag caagagggat ctcgtgggac 121 acggggcctt cgccgtggtc ttccgggggc ggcaccgcca gaaaactgat tgggaggtag 181 ctattaaaag tattaataaa aagaacttgt caaaatcaca aatactgctt ggaaaggaaa 241 ttaaaatctt aaaggaactt cagcatgaaa atattgtagc actctatgat gttcaggaat 301 tacccaactc tgtctttttg gtgatggagt attgcaatgg tggagacctc gcagattatt 361 tgcaagcgaa agggactctc agtgaagaca cgatcagagt gtttctgcat cagattgctg 421 ctgccatgcg aatcctgcac agcaaaggaa tcatccacag agatctcaaa ccacagaaca 481 tcttgctgtc ctatgccaat cgcagaaaat caagtgtcag tggtattcgc atcaaaatag 541 cggattttgg ttttgctcgt tacctacata gtaacatgat ggctgcaaca ctgtgtggat 601 ccccgatgta catggctcct gaggttatta tgtctcaaca ttatgatgct aaggctgact 661 tgtggagcat aggaacagtg atataccaat gcctagttgg aaaaccacct tttcagaagc 721 gttttttagc catccttttc ttgagcaagg tccagtaaaa aaatcttgcc cagttccagt 781 gcccatgtat tctggttctg tctctggaag ctcctgtggc agctctccat cttgtcgttt 841 tgcttctcca ccagtgatat gccaatgggg actgctggca gacgtgcttc aaatgaattc 901 ttggtgtgtg gagggcagtg tcagcctact gtgtcacctc acagcgaaac agcaccaatt 961 ccagttccta ctcaaataag gaattatcag cgcatagagc agaatcttac atctactgcc 1021 agctcaggca caaatgtaca tggttctcca agatctgcag tggtacgaag gtccaacacc 1081 agccccatgg gcttcctccg gccgggatca tgctccccag taccagcaga cacagcacag 1141 acagttggac gaaggctctc cactgggtct tctaggcctt actcaccttc ccctttggtt 1201 ggtaccattc ctgagcaatt cagtcagtgc tgctgtgggc atcctcaggg ccatgactcc 1261 aggagtagaa actcctcagg ttctccagtg ccacaagctc agtccccaca gtctctctta 1321 tcgggtgcta gactgcagag cgcccccacc ctcactgaca tctatcagaa caagcagaag 1381 ctcagaaaac agcactctga ccccgtgtgc ccatcccata ctggggctgg gtacagctac 1441 tcgcctcagc ccagtcggcc tggcagcctt ggaacttctc ccaccaagca cttggggtcc 1501 tctccacgga gttctgactg gttctttaaa actcctttgc caacaatcat tggctctcct 1561 actaagacca cagctccttt caaaatccct aaaactcaag catcttccaa cctgttagcc 1621 ttggttactc gtcatgggcc tgctgaagaa cagtcgaaag atgggaatga gccacgggaa 1681 tgtgcccatt gcctcttagt gcaaggaagt gagaggcagc gggccgagca gcagagcaag 1741 gcagtgtttg gcagatctgt cagtaccggg aagttatcag atcaacaagg aaagactcct 1801 atatgtcgac atcagggcag cacagacagt ttaaatacag aacgaccaat ggatatagct 1861 ccggcaggag cctgtggtgg tgttctggca cctcctgcag gtacagcagc aagttccaag 1921 gctgtcctct tcactgtagg gtctcctcca cacagtgcag cagcccccac ttgtacccac 1981 atgttccttc gaacaagaac aacctcagcg ggaacacaca gacaccttac gccatctgaa 2041 tgtgatgctg atgttcactg agtgtgtgct ggacctgaca gccatgaggg gaggaaaccc 2101 tgagctgtgc acatctgctg tgtccttgta ccagatccag gagagtgtgg tggtggacca 2161 gatcagtcag ctgagcaaag actgggggcg ggtggagcag ctggtgttgt acatgaaagc 2221 agcacagctg cttgcggctt ctctgcatct tgccaaagcc cagatcaagt ccgggaaact 2281 gagcccatcc acagctgtga aacaagttgt caagaatcTG AACGAACGAT ATAAATTCTG 2341 CATCACCATG TGCAAGAAAC TTACAGAAAA GCTGAATCGA TTCTTCTCTG ACAAACAGAG 2401 GTTTATTGAT GAAGTCAACA GTGTGACTGC AGAGAAACTC ATCTATAATT GTGCTGTAGA 2461 AATGGTTCAG TCTGCAGCCC TGGATGAGAT GTTTCAGCAG ACCGAAGATA TTGTTTATCG 2521 CTATCATAAG GCAGCCCTTC TTTTGGAAGG CCTAAGTAGG ATTCTACAGG ACCCTGCAGA 2581 TATTGAAAAT GTGCATAAAT ATAAATGTAG TATTGAGAGA AGACTGTCGG CGCTCTGCCA 2641 TAGCACCGCA ACCGTGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 2701 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 2761 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATACACTTG TACATAAGTT 2821 AGCTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt