Transcript: Mouse NM_015781.4

Mus musculus nucleosome assembly protein 1-like 1 (Nap1l1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Nap1l1 (53605)
Length:
1529
CDS:
181..1356

Additional Resources:

NCBI RefSeq record:
NM_015781.4
NBCI Gene record:
Nap1l1 (53605)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_015781.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000092889 CGATCCAGACTATGACCCAAA pLKO.1 1299 CDS 100% 4.050 5.670 N Nap1l1 n/a
2 TRCN0000427064 GAAGCACTTGAAAGATATTAA pLKO_005 759 CDS 100% 15.000 10.500 N Nap1l1 n/a
3 TRCN0000433102 GTCTGAACTTGATCAAGATTT pLKO_005 207 CDS 100% 13.200 9.240 N Nap1l1 n/a
4 TRCN0000430279 AGATCATTAATGCAATCTATG pLKO_005 536 CDS 100% 10.800 7.560 N Nap1l1 n/a
5 TRCN0000433721 AGGAACATGATGAACCTATTC pLKO_005 737 CDS 100% 10.800 7.560 N Nap1l1 n/a
6 TRCN0000148741 CCTATTCTGAAGCACTTGAAA pLKO.1 751 CDS 100% 5.625 3.938 N NAP1L1 n/a
7 TRCN0000280693 CCTATTCTGAAGCACTTGAAA pLKO_005 751 CDS 100% 5.625 3.938 N NAP1L1 n/a
8 TRCN0000092890 GCCAAATTCTATGAGGAAGTT pLKO.1 457 CDS 100% 4.950 3.465 N Nap1l1 n/a
9 TRCN0000149872 GCCAAATTCTATGAGGAAGTT pLKO.1 457 CDS 100% 4.950 3.465 N NAP1L1 n/a
10 TRCN0000280692 GCCAAATTCTATGAGGAAGTT pLKO_005 457 CDS 100% 4.950 3.465 N NAP1L1 n/a
11 TRCN0000092892 GCTCTTCAGGAAAGACTTGAT pLKO.1 331 CDS 100% 4.950 3.465 N Nap1l1 n/a
12 TRCN0000092891 AGCCTTTATTTGATAAGCGAT pLKO.1 512 CDS 100% 2.640 1.848 N Nap1l1 n/a
13 TRCN0000149610 GAAGCCAAATTCTATGAGGAA pLKO.1 454 CDS 100% 2.640 1.848 N NAP1L1 n/a
14 TRCN0000149921 GCCAAGATTGAAGATGAGAAA pLKO.1 628 CDS 100% 4.950 2.970 N NAP1L1 n/a
15 TRCN0000280626 GCCAAGATTGAAGATGAGAAA pLKO_005 628 CDS 100% 4.950 2.970 N NAP1L1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015781.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06614 pDONR223 100% 93.4% 98.2% None (many diffs) n/a
2 ccsbBroad304_06614 pLX_304 0% 93.4% 98.2% V5 (many diffs) n/a
3 TRCN0000465323 CACTGGCTCCTTCTACCGCATGGG pLX_317 33.5% 93.4% 98.2% V5 (many diffs) n/a
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