Transcript: Human NM_016281.4

Homo sapiens TAO kinase 3 (TAOK3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
TAOK3 (51347)
Length:
4346
CDS:
454..3150

Additional Resources:

NCBI RefSeq record:
NM_016281.4
NBCI Gene record:
TAOK3 (51347)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148231 AACAGATGTCAGGTTATAAG pXPR_003 CGG 1392 52% 15 0.5109 TAOK3 TAOK3 76737
2 BRDN0001149287 CCTAATACTATTGAGTACAA pXPR_003 AGG 260 10% 5 0.4911 TAOK3 TAOK3 76735
3 BRDN0001149068 GTATTCATAAGGGATGAGGC pXPR_003 GGG 1217 45% 14 0.2984 TAOK3 TAOK3 76734
4 BRDN0001148268 TTCATTAGACTGTAACGTTG pXPR_003 GGG 718 27% 10 -0.1520 TAOK3 TAOK3 76736
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016281.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273335 TAACTAGCTGCTGCATTATTT pLKO_005 3599 3UTR 100% 15.000 21.000 N TAOK3 n/a
2 TRCN0000242196 AGCGCTTTGCCACGATCAAAT pLKO_005 1751 CDS 100% 13.200 18.480 N Taok3 n/a
3 TRCN0000273361 AGCGCTTTGCCACGATCAAAT pLKO_005 1751 CDS 100% 13.200 18.480 N TAOK3 n/a
4 TRCN0000001527 GAGTACAATAAGAGGCGAGAA pLKO.1 2515 CDS 100% 4.050 3.240 N TAOK3 n/a
5 TRCN0000273336 ATGACGAAAGCACAATCAATT pLKO_005 1601 CDS 100% 13.200 9.240 N TAOK3 n/a
6 TRCN0000382396 GAGCGGATCTCCAAACATAAA pLKO_005 2191 CDS 100% 13.200 9.240 N TAOK3 n/a
7 TRCN0000380562 TGGACTAGCCTACCTACATTC pLKO_005 852 CDS 100% 10.800 7.560 N TAOK3 n/a
8 TRCN0000196860 GCCGATCTATTCTACAAAGAT pLKO.1 487 CDS 100% 5.625 3.938 N TAOK3 n/a
9 TRCN0000001525 CCGTCTCTTCTATTCACAGTA pLKO.1 3372 3UTR 100% 4.950 3.465 N TAOK3 n/a
10 TRCN0000001529 AGCGAGAGAATAAAGAACCTA pLKO.1 3025 CDS 100% 3.000 2.100 N TAOK3 n/a
11 TRCN0000001526 GCAGCAACCATTCCATTCCAA pLKO.1 1487 CDS 100% 3.000 2.100 N TAOK3 n/a
12 TRCN0000194986 CGTCACAGTATTGATGTGATT pLKO.1 3395 3UTR 100% 0.495 0.347 N TAOK3 n/a
13 TRCN0000273334 GGAACAGATGTCAGGTTATAA pLKO_005 1827 CDS 100% 15.000 9.000 N TAOK3 n/a
14 TRCN0000001528 GAAAGGCAAGAGCGAGAGATT pLKO.1 3049 CDS 100% 4.950 2.970 N TAOK3 n/a
15 TRCN0000273337 GAAAGGCAAGAGCGAGAGATT pLKO_005 3049 CDS 100% 4.950 2.970 N TAOK3 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016281.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15065 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15065 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000465631 GATCACGGGGCAACCCTTCGATAC pLX_317 13.2% 100% 100% V5 n/a
4 TRCN0000487785 CCTCCAGGGTGACACTCCAAACGT pLX_317 10.6% 99.9% 99.7% V5 (not translated due to prior stop codon) 140G>A;1231G>A n/a
5 TRCN0000488252 CCATAATCCTTCGCATTACGCTCA pLX_317 13.1% 99.8% 99.6% V5 140G>A;1231G>A;2694_2695insG n/a
Download CSV