Transcript: Mouse NM_030133.3

Mus musculus S1 RNA binding domain 1 (Srbd1), mRNA.

Source:
NCBI, updated 2017-04-17
Taxon:
Mus musculus (mouse)
Gene:
Srbd1 (78586)
Length:
3530
CDS:
56..3001

Additional Resources:

NCBI RefSeq record:
NM_030133.3
NBCI Gene record:
Srbd1 (78586)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_030133.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216912 GATTCCTATCCGGTTCATAAC pLKO.1 2848 CDS 100% 10.800 15.120 N Srbd1 n/a
2 TRCN0000294430 CTTTGTGGGAGTGGATATTAA pLKO_005 2152 CDS 100% 15.000 10.500 N SRBD1 n/a
3 TRCN0000217868 GCATGTGTCTGCTCCATATAA pLKO.1 967 CDS 100% 15.000 10.500 N Srbd1 n/a
4 TRCN0000191134 CCACTTTATTGAGGTGTTATT pLKO.1 3338 3UTR 100% 13.200 9.240 N Srbd1 n/a
5 TRCN0000191882 GCAGTTTCTGTCTTTGTAATT pLKO.1 3181 3UTR 100% 13.200 9.240 N Srbd1 n/a
6 TRCN0000200707 GCCACTTTATTGAGGTGTTAT pLKO.1 3337 3UTR 100% 13.200 9.240 N Srbd1 n/a
7 TRCN0000216218 CTTAAGAGAAGTTCGTCAAAC pLKO.1 820 CDS 100% 10.800 7.560 N Srbd1 n/a
8 TRCN0000189668 CCATCCGGAATCCTATGACAT pLKO.1 2497 CDS 100% 4.950 3.465 N Srbd1 n/a
9 TRCN0000191921 GAACAGTTGAAGAAGGTGAAA pLKO.1 2276 CDS 100% 4.950 3.465 N Srbd1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030133.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12167 pDONR223 100% 53.6% 55.4% None (many diffs) n/a
2 ccsbBroad304_12167 pLX_304 0% 53.6% 55.4% V5 (many diffs) n/a
3 TRCN0000470281 CGAAGGTCCAAGCGTTTCTTACAA pLX_317 18.7% 53.6% 55.4% V5 (many diffs) n/a
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