Construct: ORF ccsbBroad304_12167

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002228.1_s304c1, BRDN0000410255
Derived from:
ccsbBroadEn_12167
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRBD1 (55133)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12167
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55133 SRBD1 S1 RNA binding domain 1 XM_017004418.1 79.6% 78.7% (many diffs)
2 human 55133 SRBD1 S1 RNA binding domain 1 NM_018079.5 62.3% 62.3% 1_1125del
3 human 55133 SRBD1 S1 RNA binding domain 1 XM_011532946.2 60.3% 59.6% (many diffs)
4 human 55133 SRBD1 S1 RNA binding domain 1 XR_001738804.2 56.3% 1_1201del;1950_1993del;2995_2996ins110
5 human 55133 SRBD1 S1 RNA binding domain 1 XR_001738805.2 48.1% 1_1201del;2039_2040ins83;2979_3611del
6 human 55133 SRBD1 S1 RNA binding domain 1 XR_001738806.2 47.8% 1_1201del;1949_1950ins92;2970_3602del
7 human 55133 SRBD1 S1 RNA binding domain 1 XR_002959310.1 47.5% (many diffs)
8 human 55133 SRBD1 S1 RNA binding domain 1 XR_939693.3 45.6% 1_1201del;1949_1950ins175;2887_3519del
9 mouse 78586 Srbd1 S1 RNA binding domain 1 XM_011246731.2 81.9% 84.2% (many diffs)
10 mouse 78586 Srbd1 S1 RNA binding domain 1 NM_030133.3 53.6% 55.4% (many diffs)
11 mouse 78586 Srbd1 S1 RNA binding domain 1 XM_011246728.1 52.8% 54.5% (many diffs)
12 mouse 78586 Srbd1 S1 RNA binding domain 1 XR_385394.3 33.6% (many diffs)
13 mouse 78586 Srbd1 S1 RNA binding domain 1 XM_017317728.1 28.2% 29% (many diffs)
14 mouse 78586 Srbd1 S1 RNA binding domain 1 XM_011246730.2 22.8% 23.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1926
ORF length:
1860
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat tgctaaagac aaagacacgc ttgacttcat tcggaacttg tgccagaaga
121gacatgtttg tatccagtca tctctggcaa aagtatcctc aaaaaaggta aatgagaaag
181atgttgataa gtttctgctc taccagcatt tttcctgcaa cataagaaac attcaccatc
241atcagattct ggcaattaac cgtggagaaa atttgaaggt actgacggtt aaggtcaata
301tttctgatgg agtgaaggat gaattctgta ggtggtgcat ccaaaacagg tggagaccac
361gtagctttgc aaggccagag ttaatgaaga tcttatataa ttcactgaat gattccttta
421aacgccttat ttatcctctt ctctgtagag aattcagagc caaactaaca tcagatgcag
481agaaggaatc agtaatgatg tttggacgga accttcgtca gctcctttta acaagccctg
541ttccagggcg caccttaatg ggagtggatc ctggttataa acatggttgc aaattagcta
601taatttctcc tactagtcag atacttcata ctgatgtggt ttacttgcat tgtggacaag
661gcttccgaga ggcggagaaa ataaagacac ttttgctgaa tttcaactgc agcacagtag
721tgattggaaa tggaactgcc tgcagggaaa cagaagctta ctttgctgac ctgataatga
781agaattattt tgcaccactg gatgttgttt actgtatcgt cagtgaagca ggagcatcaa
841tctacagtgt cagccctgaa gctaacaaag agatgccagg gctggaccct aatttgagaa
901gtgcagtttc catagcaagg cgtgtacaag atccattagc tgagctagtg aaaattgagc
961caaagcacat tggagttgga atgtatcagc atgacgtatc ccagacttta ctcaaggcaa
1021cactggacag tgttgtagaa gaatgtgtca gctttgtggg agtggatatt aacatctgtt
1081cagaagtttt gttaaggcat attgcaggac tcaatgccaa cagggccaaa aatattattg
1141aatggcgaga gaaaaatgga ccctttatca accgagaaca gctgaagaaa gtgaaagggc
1201tgggcccaaa atccttccaa cagtgtgctg gcttcatcag aatcaaccag gattatatcc
1261gaacgttttg cagtcagcaa actgaaactt caggccaaat tcaaggagtt gctgtgacat
1321cttcagcaga cgttgaggtc acaaatgaga agcagggcaa aaagaagagc aaaactgcag
1381tgaatgtttt actgaagcca aatcctttgg accaaacttg tattcatcca gaatcatatg
1441acatagcaat gaggtttttg tcatccattg gagggacact gtatgaggtt ggaaagcctg
1501aaatgcaaca aaaaataaat tcattccttg aaaaggaagg aatggagaaa attgcagaaa
1561gattgcaaac aacagtacac accttacagg tcatcataga tggtctcagc cagcctgaaa
1621gctttgactt tcgaacagat tttgataaac ctgatttcaa gagaagcata gtatgcctgg
1681aagatctgca gattgggaca gttcttacag gcaaagttga gaatgccact ctctttggaa
1741tttttgtgga tataggagtg gggaaatctg ggctgattcc catacgaaat gtaacagaag
1801caaaactttc aaaaacaaag aagagaagaa gccttggact gggccccgga gaaagagtgg
1861aagtccaagt actcaacatt gacatccccc gatctaggat tactctggac ctcattcggg
1921tgttatgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1981gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2041acctctggat ta

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