Construct: ORF TRCN0000470281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002228.1_s317c1
- Derived from:
- ccsbBroadEn_12167
- DNA Barcode:
- CGAAGGTCCAAGCGTTTCTTACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRBD1 (55133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470281
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XM_017004418.1 | 79.6% | 78.7% | (many diffs) |
| 2 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | NM_018079.5 | 62.3% | 62.3% | 1_1125del |
| 3 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XM_011532946.2 | 60.3% | 59.6% | (many diffs) |
| 4 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XR_001738804.2 | 56.3% | 1_1201del;1950_1993del;2995_2996ins110 | |
| 5 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XR_001738805.2 | 48.1% | 1_1201del;2039_2040ins83;2979_3611del | |
| 6 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XR_001738806.2 | 47.8% | 1_1201del;1949_1950ins92;2970_3602del | |
| 7 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XR_002959310.1 | 47.5% | (many diffs) | |
| 8 | human | 55133 | SRBD1 | S1 RNA binding domain 1 | XR_939693.3 | 45.6% | 1_1201del;1949_1950ins175;2887_3519del | |
| 9 | mouse | 78586 | Srbd1 | S1 RNA binding domain 1 | XM_011246731.2 | 81.9% | 84.2% | (many diffs) |
| 10 | mouse | 78586 | Srbd1 | S1 RNA binding domain 1 | NM_030133.3 | 53.6% | 55.4% | (many diffs) |
| 11 | mouse | 78586 | Srbd1 | S1 RNA binding domain 1 | XM_011246728.1 | 52.8% | 54.5% | (many diffs) |
| 12 | mouse | 78586 | Srbd1 | S1 RNA binding domain 1 | XR_385394.3 | 33.6% | (many diffs) | |
| 13 | mouse | 78586 | Srbd1 | S1 RNA binding domain 1 | XM_017317728.1 | 28.2% | 29% | (many diffs) |
| 14 | mouse | 78586 | Srbd1 | S1 RNA binding domain 1 | XM_011246730.2 | 22.8% | 23.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1926
- ORF length:
- 1860
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgctaaagac aaagacacgc ttgacttcat tcggaacttg tgccagaaga 121 gacatgtttg tatccagtca tctctggcaa aagtatcctc aaaaaaggta aatgagaaag 181 atgttgataa gtttctgctc taccagcatt tttcctgcaa cataagaaac attcaccatc 241 atcagattct ggcaattaac cgtggagaaa atttgaaggt actgacggtt aaggtcaata 301 tttctgatgg agtgaaggat gaattctgta ggtggtgcat ccaaaacagg tggagaccac 361 gtagctttgc aaggccagag ttaatgaaga tcttatataa ttcactgaat gattccttta 421 aacgccttat ttatcctctt ctctgtagag aattcagagc caaactaaca tcagatgcag 481 agaaggaatc agtaatgatg tttggacgga accttcgtca gctcctttta acaagccctg 541 ttccagggcg caccttaatg ggagtggatc ctggttataa acatggttgc aaattagcta 601 taatttctcc tactagtcag atacttcata ctgatgtggt ttacttgcat tgtggacaag 661 gcttccgaga ggcggagaaa ataaagacac ttttgctgaa tttcaactgc agcacagtag 721 tgattggaaa tggaactgcc tgcagggaaa cagaagctta ctttgctgac ctgataatga 781 agaattattt tgcaccactg gatgttgttt actgtatcgt cagtgaagca ggagcatcaa 841 tctacagtgt cagccctgaa gctaacaaag agatgccagg gctggaccct aatttgagaa 901 gtgcagtttc catagcaagg cgtgtacaag atccattagc tgagctagtg aaaattgagc 961 caaagcacat tggagttgga atgtatcagc atgacgtatc ccagacttta ctcaaggcaa 1021 cactggacag tgttgtagaa gaatgtgtca gctttgtggg agtggatatt aacatctgtt 1081 cagaagtttt gttaaggcat attgcaggac tcaatgccaa cagggccaaa aatattattg 1141 aatggcgaga gaaaaatgga ccctttatca accgagaaca gctgaagaaa gtgaaagggc 1201 tgggcccaaa atccttccaa cagtgtgctg gcttcatcag aatcaaccag gattatatcc 1261 gaacgttttg cagtcagcaa actgaaactt caggccaaat tcaaggagtt gctgtgacat 1321 cttcagcaga cgttgaggtc acaaatgaga agcagggcaa aaagaagagc aaaactgcag 1381 tgaatgtttt actgaagcca aatcctttgg accaaacttg tattcatcca gaatcatatg 1441 acatagcaat gaggtttttg tcatccattg gagggacact gtatgaggtt ggaaagcctg 1501 aaatgcaaca aaaaataaat tcattccttg aaaaggaagg aatggagaaa attgcagaaa 1561 gattgcaaac aacagtacac accTTACAGG TCATCATAGA TGGTCTCAGC CAGCCTGAAA 1621 GCTTTGACTT TCGAACAGAT TTTGATAAAC CTGATTTCAA GAGAAGCATA GTATGCCTGG 1681 AAGATCTGCA GATTGGGACA GTTCTTACAG GCAAAGTTGA GAATGCCACT CTCTTTGGAA 1741 TTTTTGTGGA TATAGGAGTG GGGAAATCTG GGCTGATTCC CATACGAAAT GTAACAGAAG 1801 CAAAACTTTC AAAAACAAAG AAGAGAAGAA GCCTTGGACT GGGCCCCGGA GAAAGAGTGG 1861 AAGTCCAAGT ACTCAACATT GACATCCCCC GATCTAGGAT TACTCTGGAC CTCATTCGGG 1921 TGTTATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1981 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2041 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGAAGGTCC AAGCGTTTCT TACAAACGCG 2101 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt