Transcript: Human NM_030627.4

Homo sapiens cytoplasmic polyadenylation element binding protein 4 (CPEB4), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
CPEB4 (80315)
Length:
9418
CDS:
1386..3575

Additional Resources:

NCBI RefSeq record:
NM_030627.4
NBCI Gene record:
CPEB4 (80315)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_030627.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000153850 CCGATAGCTCTCTGCTTATTA pLKO.1 2620 CDS 100% 15.000 21.000 N CPEB4 n/a
2 TRCN0000151922 CGATAGCTCTCTGCTTATTAA pLKO.1 2621 CDS 100% 15.000 21.000 N CPEB4 n/a
3 TRCN0000150754 GCGTTATGTGTTGAACAGTAT pLKO.1 4972 3UTR 100% 4.950 6.930 N CPEB4 n/a
4 TRCN0000152631 GCATATTTCATTCCGCTGGAA pLKO.1 3551 CDS 100% 2.640 3.696 N CPEB4 n/a
5 TRCN0000156566 GCGATGATAATGGATCGGCTA pLKO.1 3174 CDS 100% 2.160 3.024 N CPEB4 n/a
6 TRCN0000256164 TACCATTAAAGGTCGTCTAAA pLKO_005 2582 CDS 100% 0.000 0.000 N CPEB4 n/a
7 TRCN0000256163 AGCTATTGGTGTACCTATAAT pLKO_005 6031 3UTR 100% 15.000 10.500 N CPEB4 n/a
8 TRCN0000153725 CCCTGCGCTTTGGTATAATAT pLKO.1 6757 3UTR 100% 15.000 10.500 N CPEB4 n/a
9 TRCN0000256162 AGAGTTCACTCATTGACATAA pLKO_005 2545 CDS 100% 13.200 9.240 N CPEB4 n/a
10 TRCN0000156565 GCTGCCTCATTTGGCGAATAA pLKO.1 2195 CDS 100% 13.200 9.240 N CPEB4 n/a
11 TRCN0000256161 TCCCTGCTGCTTCGGCTAATA pLKO_005 1969 CDS 100% 13.200 9.240 N CPEB4 n/a
12 TRCN0000151279 CTCATAAAGCTGAGAGCAAAT pLKO.1 2893 CDS 100% 10.800 7.560 N CPEB4 n/a
13 TRCN0000158083 CCAGTGTTGACAGGGTTTGAT pLKO.1 1797 CDS 100% 5.625 3.938 N CPEB4 n/a
14 TRCN0000152730 CCTCATAAAGCTGAGAGCAAA pLKO.1 2892 CDS 100% 4.950 3.465 N CPEB4 n/a
15 TRCN0000198916 GAAGCTGGAATACTGCCTGAA pLKO.1 1692 CDS 100% 4.050 2.835 N Cpeb4 n/a
16 TRCN0000152863 GCTACCCATAACATTCAGGAT pLKO.1 1581 CDS 100% 2.640 1.848 N CPEB4 n/a
17 TRCN0000177316 GCAAAGCAATACTGGGAATAA pLKO.1 1415 CDS 100% 13.200 7.920 N Cpeb4 n/a
18 TRCN0000150825 GCTGTTGGAAAGACTTGATAA pLKO.1 6856 3UTR 100% 13.200 7.920 N CPEB4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030627.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12692 pDONR223 100% 44.1% 44.1% None 1_1146del;1206_1280del n/a
2 ccsbBroad304_12692 pLX_304 0% 44.1% 44.1% V5 1_1146del;1206_1280del n/a
3 TRCN0000491501 CTTGCGGGTGCGAGCAGGAATTAG pLX_317 35.5% 44.1% 44.1% V5 1_1146del;1206_1280del n/a
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